A5049702760- Brain Metastases and Treatment
- Prostate Cancer Treatment and Research
- Peptidase Inhibition and Analysis
- Glioma Diagnosis and Treatment
- Lung Cancer Treatments and Mutations
- Radiopharmaceutical Chemistry and Applications
- Cancer Genomics and Diagnostics
- Semiconductor materials and devices
- Prostate Cancer Diagnosis and Treatment
- Cancer, Lipids, and Metabolism
- Lung Cancer Research Studies
- DNA Repair Mechanisms
- Estrogen and related hormone effects
- Photoreceptor and optogenetics research
- Genetic factors in colorectal cancer
- Receptor Mechanisms and Signaling
- Economic and Financial Impacts of Cancer
- Effects of Radiation Exposure
- Advanced Fluorescence Microscopy Techniques
- Innovations in Medical Education
- Cell Image Analysis Techniques
- Light effects on plants
- FOXO transcription factor regulation
- Health Sciences Research and Education
- Health Systems, Economic Evaluations, Quality of Life
Memorial Sloan Kettering Cancer Center
2013-2025
Rutgers, The State University of New Jersey
2015-2021
Johnson University
2020-2021
Princeton University
2017-2020
Rutgers New Jersey Medical School
2015
Albert Einstein College of Medicine
2013
UNC Lineberger Comprehensive Cancer Center
2013
University of North Carolina at Chapel Hill
2013
Columbia University
2013
Abstract We demonstrate that the androgen receptor (AR) regulates a transcriptional program of DNA repair genes promotes prostate cancer radioresistance, providing potential mechanism by which deprivation therapy synergizes with ionizing radiation. Using model castration-resistant cancer, we show second-generation antiandrogen results in downregulation genes. Next, primary cancers display significant spectrum AR output, correlates expression set RNA-seq and ChIP-seq, define these are both...
Abstract A growing number of optogenetic tools have been developed to reversibly control binding between two engineered protein domains. In contrast, relatively few confer light-switchable a generic target interest. Such capability would offer substantial advantages, enabling photoswitchable endogenous proteins in cells or light-based purification vitro. Here, we report the development opto-nanobodies (OptoNBs), versatile class chimeric whose interest can be enhanced inhibited upon blue...
Double-strand break (DSB) repair pathways are critical for the maintenance of genomic integrity and prevention tumorigenesis in mammalian cells. Here, we present development validation a novel assay to measure mutagenic non-homologous end-joining (NHEJ) living cells, which is inversely related canonical NHEJ based on sequence-altering single site-specific DSB at an intrachromosomal locus. We have combined this with established homologous recombination (HR) such that both can be monitored...
Most cancer therapies involve a component of treatment that inflicts DNA damage in tumor cells, such as double-strand breaks (DSBs), which are considered the most serious threat to genomic integrity. Complex systems have evolved repair these lesions, and successful DSB is essential for cell survival after exposure ionizing radiation (IR) other DNA-damaging agents. As such, inhibition potentially efficacious strategy chemo- radiosensitization. Homologous recombination (HR) nonhomologous...
ABSTRACT Background Higher rates of radiation therapy (RT) noncompletion may be associated with certain demographic groups in patients prostate cancer (PC). We examined disparities and receipt shorter RT regimens among disaggregated Asian American Pacific Islander the US. Methods performed a retrospective cohort analysis all diagnosed localized PC (2004–2017) National Cancer Database who identified as White, East Asian, Southeast Islander, or South were treated definitive RT. The two primary...
Proton craniospinal irradiation (pCSI) is a promising treatment for patients with solid tumor leptomeningeal metastasis (LM). We hypothesize that genetic characteristics before and changes resulting after pCSI will reflect clinical response to pCSI. analyzed the cerebrospinal fluid (CSF) circulating DNA (ctDNA) from receiving LM explored variations associated response.We subjected CSF 14 cell-free sequencing using targeted-sequencing panel. In parallel, plasma ctDNA primary tumors were...
// Laura Barrio-Real 1, * , Cynthia Lopez-Haber Victoria Casado-Medrano 1 Alexander G. Goglia 2 Jared E. Toettcher Maria J. Caloca 3 and Marcelo Kazanietz Department of Systems Pharmacology Translational Therapeutics, Perelman School Medicine, University Pennsylvania, Philadelphia, PA 19104-6160, USA Molecular Biology, Princeton University, Princeton, NJ 08544-1014, Instituto de Biología y Genética Molecular, Consejo Superior Investigaciones Científicas, Universidad Valladolid, 47003 Spain...
Abstract Introduction Alterations in forkhead box A1 (FOXA1), a pioneer transcription factor, are associated with poor prognosis breast cancer (BC) and prostate (PC). We characterized FOXA1 genomic alterations their clinical impacts large pan-cancer cohort from the AACR GENIE database. Methods were across >87,000 samples >30 types for primary metastatic tumors alongside patient characteristics outcomes. queried MSK-MET (a subset), allowing definition of hazard ratios (HRs)...
Abstract A growing number of optogenetic tools have been developed to control binding between two engineered protein domains. In contrast, relatively few confer light-switchable a generic target interest. Such capability would offer substantial advantages, enabling photoswitchable endogenous proteins in vivo or light-based purification vitro . Here, we report the development opto-nanobodies (OptoNBs), versatile class chimeric whose interest can be enhanced inhibited upon blue light...
This study aimed to define how the coronavirus disease of 2019 (COVID-19) pandemic affected role, timing, and delivery radiation therapy (RT) in a high-prevalence region at height initial U.S. outbreak.
Complex, time-varying responses have been observed widely in cell signaling, but how specific dynamics are generated or regulated is largely unknown. One major obstacle has that high-throughput screens for identifying pathway components typically incompatible with the live-cell assays used to monitor dynamics. Here, we address this challenge by performing a drug screen altered Erk signaling primary mouse keratinocytes. We screened library of 429 kinase inhibitors, monitoring activity over 5...
Microscopic polyangiitis (MPA) is an idiopathic autoimmune disease characterized by systemic vasculitis. While the lungs and kidneys are major organs affected MPA, it known to involve multiple organ systems throughout body. Temporal artery involvement a very rare finding in MPA. This report presents patient whose initial presentation was consistent with giant cell arteritis but ultimately found have microscopic polyangiitis. It highlights importance of considering alternative types...
Petabytes of increasingly complex and multidimensional live cell tissue imaging data are generated every year. These videos hold large promise for understanding biology at a deep fundamental level, as they capture single-cell multicellular events occurring over time space. However, the current modalities analysis mining these scattered user-specific, preventing more unified analyses from being performed different datasets obscuring possible scientific insights. Here, we propose pipeline...
Methods Nutrition Master's students manually reviewed the slides from every lecture in 18 month pre‐clinical medical curriculum at Columbia University College of Physicians & Surgeons (P&S) to identify content related teaching objectives defined Curriculum Guide for Training (NCGTP), a former NIH‐funded program improve nutrition education. An analogous review ten representative NCGTP was then performed using KnowledgeMap. Satisfactory KnowledgeMap search parameters unique each...
Objective To assess a novel scoring system for evaluating nutrition content in patient write‐ups performed by Columbia University medical students. Methods A binary (inclusion scored one point, omission zero) composed of 8 nutrition‐related criteria pertinent inclusion all assessments (e.g. height, weight, BMI, weight history, diet, supplements, etc) was created. evaluate the system, Nutrition Master's students separately read and sequential sets five write‐ups, performing active analysis,...