Robert J. Klein
A5015132763- Genetic Associations and Epidemiology
- Prostate Cancer Treatment and Research
- BRCA gene mutations in cancer
- Cancer Genomics and Diagnostics
- Prostate Cancer Diagnosis and Treatment
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Genomics and Rare Diseases
- Histone Deacetylase Inhibitors Research
- Genetic factors in colorectal cancer
- DNA Repair Mechanisms
- Genomic variations and chromosomal abnormalities
- Cancer-related gene regulation
- Multiple Myeloma Research and Treatments
- Nutrition, Genetics, and Disease
- RNA Research and Splicing
- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Computational Drug Discovery Methods
- Bioinformatics and Genomic Networks
- Advanced Battery Materials and Technologies
- Molecular Biology Techniques and Applications
- Enzyme Structure and Function
Icahn School of Medicine at Mount Sinai
2016-2025
University of South Carolina
2023-2024
Prisma Health
2024
Quality Living
2023-2024
Dartmouth College
2021-2023
Memorial Sloan Kettering Cancer Center
2012-2023
University of Arizona
2023
U-M Rogel Cancer Center
2023
Dartmouth Hospital
2022
University of Chicago
2021
Age-related macular degeneration (AMD) is a major cause of blindness in the elderly. We report genome-wide screen 96 cases and 50 controls for polymorphisms associated with AMD. Among 116,204 single-nucleotide genotyped, an intronic common variant complement factor H gene ( CFH ) strongly AMD (nominal P value <10 -7 ). In individuals homozygous risk allele, likelihood increased by 7.4 (95% confidence interval 2.9 to 19). Resequencing revealed polymorphism linkage disequilibrium allele...
A novel method is presented whereby the parameters quantifying conductivity of an ionomer can be extracted from phenomenon electrode polarization in dielectric loss and tanδ planes. Mobile ion concentrations mobilities were determined for a poly(ethylene oxide)-based sulfonated with Li+, Na+, Cs+ cations. The validity model was confirmed by examining effects sample thickness temperature. Vogel-Fulcher-Tammann (VFT)-type temperature dependence found to arise Arrhenius concentration VFT...
Abstract The standard of care for first-tier clinical investigation the aetiology congenital malformations and neurodevelopmental disorders is chromosome microarray analysis (CMA) copy-number variations (CNVs), often followed by gene(s)-specific sequencing searching smaller insertion–deletions (indels) single-nucleotide variant (SNV) mutations. Whole-genome (WGS) has potential to capture all classes genetic variation in one experiment; however, diagnostic yield mutation detection WGS...
We performed a three-phase genome-wide association study (GWAS) using cases and controls from genetically isolated population, Ashkenazi Jews (AJ), to identify loci associated with breast cancer risk. In the first phase, we compared allele frequencies of 150,080 SNPs in 249 high-risk, BRCA1/2 mutation-negative AJ familial 299 cancer-free chi(2) Cochran-Armitage trend tests. second genotyped 343 123 regions most significantly stage 1, including 4 FGFR2 region, 950 consecutive 979 age-matched...
Abstract ENCODE comprises thousands of functional genomics datasets, and the encyclopedia covers hundreds cell types, providing a universal annotation for genome interpretation. However, particular applications, it may be advantageous to use customized annotation. Here, we develop such custom by leveraging advanced assays, as eCLIP, Hi-C, whole-genome STARR-seq on number data-rich types. A key aspect this is comprehensive experimentally derived networks both transcription factors RNA-binding...
Despite the successes of immunotherapy in cancer treatment over recent decades, less than <10%–20% cases have demonstrated durable responses from immune checkpoint blockade. To enhance efficacy immunotherapies, combination therapies suppressing multiple evasion mechanisms are increasingly contemplated. better understand cell surveillance and diverse tumor tissues, we comprehensively characterized landscape more 1,000 tumors across ten different cancers using CPTAC pan-cancer proteogenomic...
For many RNA molecules, secondary structure rather than primary sequence is the evolutionarily conserved feature. No programs have yet been published that allow searching a database for homologs of single molecule on basis structure. We developed program, RSEARCH, takes with its and utilizes local alignment algorithm to search homologous RNAs. this purpose, we series base pair nucleotide substitution matrices sequences called RIBOSUM matrices. RSEARCH reports statistical confidence each hit...
We developed a new empirical scoring function, HYDE, for the evaluation of protein-ligand complexes. HYDE estimates binding free energy based on two terms dehydration and hydrogen bonding only. The essential feature this function is integrated use log P-derived atomic increments prediction energy. Taking atoms within interface into account shows that some contribute favorably to overall score, while others unfavorably. For instance, bond functions are penalized if they dehydrated unless can...
Abstract To investigate large structural clonal mosaicism of chromosome X, we analysed the SNP microarray intensity data 38,303 women from cancer genome-wide association studies (20,878 cases and 17,425 controls) detected 124 mosaic X events >2 Mb in 97 (0.25%) women. Here show rates for X-chromosome are four times higher than mean autosomal rates; more often include entire participants with likely harbour events. frequency increases age (0.11% 50-year olds; 0.45% 75-year olds), as...