A5088853072- Immune Response and Inflammation
- NF-κB Signaling Pathways
- Cell Adhesion Molecules Research
- Blood Coagulation and Thrombosis Mechanisms
- Bacillus and Francisella bacterial research
- Virus-based gene therapy research
- Lipid metabolism and disorders
- interferon and immune responses
- CRISPR and Genetic Engineering
- Barrier Structure and Function Studies
- RNA Interference and Gene Delivery
- Herpesvirus Infections and Treatments
- Phosphodiesterase function and regulation
- Receptor Mechanisms and Signaling
- Bacteriophages and microbial interactions
- Cellular transport and secretion
- Cytokine Signaling Pathways and Interactions
- Gene Regulatory Network Analysis
- Inflammatory Bowel Disease
- Cancer-related molecular mechanisms research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Signaling Pathways in Disease
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Angiogenesis and VEGF in Cancer
- Autoimmune and Inflammatory Disorders
National Institute on Aging
2018-2024
National Institutes of Health
2018-2024
Systems Dynamics (United States)
2018-2024
Booz Allen Hamilton (United States)
2024
University of Maryland, Baltimore
2011-2022
MRC Laboratory of Molecular Biology
2022
University of Maryland Marlene and Stewart Greenebaum Comprehensive Cancer Center
2019
Center for Vascular Biology Research
2014
Kyowa Kirin (France)
2013
Thrombomodulin (TM), recognized as an essential vessel wall cofactor of the antithrombotic mechanism, is also expressed by a wide range tumor cells. Tumor cell lines subcloned from four patients with malignant melanoma displayed negative correlation between TM expression and proliferation in vitro vivo. Overexpression wild-type decreased growth mutants altered protein C activation capacity lead to similar effect. In contrast, transfection cells mutant constructs, which portion cytoplasmic or...
A large group of viruses rely on low pH to activate their fusion proteins that merge the viral envelope with an endosomal membrane, releasing nucleocapsid. critical barrier understanding these events has been lack approaches study virus-cell membrane within acidic endosomes, natural sites virus nucleocapsid capsid entry into cytosol. Here we have investigated using highly tractable subgroup avian sarcoma and leukosis glycoprotein (EnvA)-TVA receptor system. Through labeling EnvA pseudotyped...
Integrating single-cell imaging and gene expression data illustrates how cells differentially interpret pathogenic stimuli.
// Erik W. Martin 1 , Marguerite S. Buzza Kathryn H. Driesbaugh Shihui Liu 2 Yolanda M. Fortenberry 3 Stephen Leppla Toni Antalis Center for Vascular and Inflammatory Diseases the Department of Physiology, University Maryland School Medicine, Baltimore, MD 21201, USA National Institute Allergy Infectious Diseases, Institutes Health, Bethesda, 20892, Division Pediatric Hematology, Johns Hopkins 21205, Correspondence to: Antalis, e-mail: tantalis@som.umaryland.edu Keywords: anthrax toxin,...
Technological advances are continually improving our ability to obtain more accurate views about the inner workings of biological systems. One such rapidly evolving area is single cell biology, and in particular gene expression its regulation by transcription factors response intrinsic extrinsic factors. Regarding study factors, we discuss some promises pitfalls associated with investigating how individual cells regulate through modulation factor activities. Specifically, four leading...
Liposomes are promising vehicles to deliver diagnostic and therapeutic agents cells in vivo. After uptake into by endocytosis, liposomes degraded the endolysosomal system. Consequently, encapsulated cargo molecules frequently remain sequestered endosomal compartments; this limits their usefulness many applications (e.g. gene delivery). To overcome this, various fusogenic peptides have been developed facilitate delivery of liposomally-encapsulated cytosol. One such peptide is pH-sensitive...
NF-κB is a family of heterodimers and homodimers which are generated from subunits encoded by five genes. The predominant classical dimer RelA:p50 presumed to operate as "NF-κB" in many contexts. However, there several other species exist may even be more functionally relevant forms specific cell types. Accurate characterization stimulus-specific tissue-specific repertoires fundamentally important for understanding the downstream gene regulation proteins. In vitro assays such...
Treatments for advanced and recurrent ovarian cancer remain a challenge due to lack of potent, selective, effective therapeutics. Here, we developed the basis transformative anticancer strategy based on anthrax toxin that has been engineered be selectively activated by catalytic power zymogen-activating proteases surface malignant tumor cells induce cell death. Exposure is cytotoxic lines spheroids derived from patient ascites. Preclinical studies demonstrate treatment induces regression in...
Abstract Targeted knockout, mutations, or knock-in of genomic DNA fragments in model organisms have been used widely for functional and cell-tracking studies. The desired genetic perturbation is often accomplished by recombination-based CRISPR/Cas9-based genome engineering. For validating the intended modification, a local region surrounding targeted locus typically examined based on enzymatic cleavage consequent length patterns, e.g. Southern analysis. Despite its wide use, this approach...
NF-κB is a transcription factor whose regulatory control reaches far beyond the immune system. In vitro studies suggest that mapping spatiotemporal complexity of signaling in primary cells and vivo essential to understanding its function health disease, but lack tools directly monitor protein components has hindered such efforts. To address this need, we generated reporter mice with endogenous RelA (p65) or c-Rel labeled distinct fluorescent proteins double knock-in both subunits. Overcoming...