A5005467119- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Identification and Quantification in Food
- Monoclonal and Polyclonal Antibodies Research
- CRISPR and Genetic Engineering
- Pharmacological Effects and Assays
- Mycotoxins in Agriculture and Food
- Wheat and Barley Genetics and Pathology
- Analytical Chemistry and Chromatography
- Advanced Biosensing Techniques and Applications
- Microbial infections and disease research
- Marine and coastal ecosystems
- Aquatic Ecosystems and Phytoplankton Dynamics
- Gold and Silver Nanoparticles Synthesis and Applications
- Mass Spectrometry Techniques and Applications
- Computational Drug Discovery Methods
- Electrochemical sensors and biosensors
- Analytical chemistry methods development
- Pesticide Residue Analysis and Safety
- Micro and Nano Robotics
- Bacillus and Francisella bacterial research
- Enzyme Structure and Function
- Protein purification and stability
- Water Treatment and Disinfection
- Enzyme Production and Characterization
South China Agricultural University
2016-2025
First People's Hospital of Yuhang District
2024
Key Laboratory of Guangdong Province
2022
South China University of Technology
2009-2015
Hokkaido University
2014-2015
Capital Normal University
2012
University of Pennsylvania
2010
State Key Laboratory of Pollution Control and Resource Reuse
2005-2009
Nanjing University
2005-2009
Northwest A&F University
2006
Salmonella species are common foodborne pathogenic bacteria. At present, most detection methods for unsuitable on-site applications because they require large instruments or complicated procedures. This study developed a novel method of the in food by combining CRISPR/Cas12a system with recombinant polymerase amplification (RPA). The optimal concentration ratio Cas12a enzyme, CRISPR RNA (crRNA) and FQ-probe is 1 : 1: 2.5. limit RPA–CRISPR/Cas12a was × 10−4 ng/μL genomic DNA (gDNA), 102...
α-Glucosidases, which catalyze the hydrolysis of α-glucosidic linkage at nonreducing end substrate, are important for metabolism α-glucosides. Halomonas sp. H11 α-glucosidase (HaG), belonging to glycoside hydrolase family 13 (GH13), only has high hydrolytic activity towards α-(1 → 4)-linked disaccharide maltose among naturally occurring substrates. Although several three-dimensional structures GH13 members have been solved, specificity and 4) recognition mechanism unclear owing a lack...
Herein, the UiO-66-NH2@quantum dot (NU66@QD) was synthesized with excellent fluorescence intensity and biocompatibility, which used to develop a multiple immunochromatographic assay (ICA) for detection of aflatoxin B1 (AFB1), fumonisin (FB1), deoxynivalenol (DON), T-2 toxins (T-2), zearalenone (ZEN) in cereals feed. Five monoclonal antibodies NU66@QD were efficiently labeled by one-step mixed method form probe. The limits proposed NU66@QD-ICA AFB1/FB1/DON/T-2/ZEN 0.04/0.28/0.25/0.09/0.08...
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The swimming behavior of the nematode Caenorhabditis elegans is investigated in aqueous solutions increasing viscosity. Detailed flow dynamics associated with nematode’s motion as well propulsive force and power are obtained using particle tracking velocimetry methods. We find that C. delivers thrusts on order a few nanonewtons. Such findings supported by values resistive theory; ratio normal to tangential drag coefficients estimated be approximately 1.4. Over range here, properties remain...
Ochratoxins A, B, and C (OTA, OTB, OTC) can be found in cereals feeds; the simultaneous detection of these ochratoxins holds a great need food safety. In this study, four antibodies raised from two ochrotoxin haptens coating antigens were compared, then sensitive broad-specificity enzyme-linked immunosorbent assay (ELISA) was established for determination three ochratoxins, where limits 0.005, 0.001, 0.001 ng/mL OTA, OTC, respectively, recoveries between 84.3% 111.7%. This developed method...
Because of the serious adulteration goat milk, rapid on-site detection milk powder is needed. In this study, CRISPR/Cas12a system combined with recombinase polymerase amplification (RPA) was employed to qualitatively detect cattle-derived components. Specific primers and crRNA were designed screened. After optimization RPA Cas system, RPA-CRISPR/Cas12a method established. The can complete identification components in 45 min, without assistant large equipment. absolute detectability assay...
A heavy-chain antibody (VHH) library against procymidone (PRM) was constructed via immunizing Bactrian camels. Through careful biopanning, seven nanobodies (Nbs) with different sequences were obtained. The variability in their performance primarily attributed to the amino acid differences complementarity-determining region 3 (CDR3), as analyzed by molecular docking. Nb exhibiting highest sensitivity, named NbFM5, biotinylated and conjugated streptavidin-labeled gold nanoparticles preserve...
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Propiconazole (PCZ) is a fungicide popularly used to prevent and control wheat rice bakanae disease, etc. However, it was recently found be illegally employed as plant regulator induce thick stems dark green leaves of Brassica campestris, famous vegetable in Guangdong, South China. Due lack available recognition molecules the target analyte, still big challenge establish rapid surveillance screening method. In this study, novel chiral hapten rationally designed, an artificial immunogen then...