A5063154094- Wound Healing and Treatments
- TGF-β signaling in diseases
- Cancer Cells and Metastasis
- Veterinary Oncology Research
- Osteoarthritis Treatment and Mechanisms
- Cellular Mechanics and Interactions
- Tendon Structure and Treatment
- Virus-based gene therapy research
- Periodontal Regeneration and Treatments
- Parasites and Host Interactions
- Cardiovascular Conditions and Treatments
- Mesenchymal stem cell research
- Helminth infection and control
- Neurogenetic and Muscular Disorders Research
- Surgical Sutures and Adhesives
- Cell Adhesion Molecules Research
- Electrospun Nanofibers in Biomedical Applications
- Bone and Dental Protein Studies
- Tissue Engineering and Regenerative Medicine
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Pluripotent Stem Cells Research
- Silk-based biomaterials and applications
- Forensic Entomology and Diptera Studies
- Planarian Biology and Electrostimulation
- Animal testing and alternatives
University of Pennsylvania
2016-2025
Penn Center for AIDS Research
2018
Philadelphia University
2014-2016
Christiana Care Health System
2016
Ludwig-Maximilians-Universität München
2013
LMU Klinikum
2013
In-Q-Tel
2013
Children's Hospital of Philadelphia
2006-2009
Penn Presbyterian Medical Center
2002
Cornell University
2002
The repair of cutaneous wounds in the postnatal animal is associated with development scar tissue. Directing cell activities to efficiently heal while minimizing tissue a major goal wound management and focus intensive research efforts. Type III collagen (Col3), expressed early granulation tissue, has been proposed play prominent role repair, although little known about its this process. To establish Col3 we examined healing excisional previously described murine model deficiency. deficiency...
Leishmaniasis causes a significant disease burden worldwide. Although Leishmania-infected patients become refractory to reinfection after resolution, effective immune protection has not yet been achieved by human vaccines. circulating Leishmania-specific T cells are known play critical role in immunity, the of memory present peripheral tissues explored. Here, we identify population skin-resident CD4+ cells. These produce IFN-γ and remain resident skin when transplanted graft onto naive mice....
Breast cancer metastasis is the leading cause of cancer-related deaths in women worldwide. Collagen tumor microenvironment plays a crucial role regulating progression. We have shown that type III collagen (Col3), component stroma, regulates myofibroblast differentiation and scar formation after cutaneous injury. During course these wound-healing studies, we noted tumors developed at higher frequency Col3(+/-) mice compared to wild-type littermate controls. We, therefore, examined effect Col3...
Tissue-resident memory T cells are required for establishing protective immunity against a variety of different pathogens, although the mechanisms mediating protection by CD4+ resident still being defined. In this study we addressed issue with population skin-resident, IFNγ-producing generated following Leishmania major infection. We previously found that recruit circulating effector to enhance immunity. Here show mediate delayed-hypersensitivity response observed in immune mice and provide...
Dogs with mucopolysaccharidosis VII (MPS VII) were injected intravenously at 2–3 days of age a retroviral vector (RV) expressing canine β-glucuronidase (cGUSB). Five animals received RV alone, and two dogs hepatocyte growth factor (HGF) before in an attempt to increase transduction efficiency. Transduced hepatocytes expanded clonally during normal liver secreted enzyme mannose 6-phosphate. Serum GUSB activity was stable for up 14 months levels the RV-treated dogs, 17 67-fold HGF/RV-treated...
Intracellular signaling triggered by bone morphogenetic proteins (BMPs) results in activated Smad complexes that regulate transcription of BMP-responsive genes. However, the low specificity binding to regulatory sequences implies additional tissue-specific factors are also needed. Runx2 (Cbfal) is a factor required for formation. We have examined role Smads and BMP induction type X collagen, which marker chondrocyte hypertrophy leading endochondral formation.Pre-hypertrophic chondrocytes...
A review of therapeutic effects in preclinical and clinical studies suggests that concordance between large animal (pig=78%), small laboratory (53%) vitro (57%) results with those observed humans is only partial. Pig models wound healing provide major advantages over other models. Since the vast majority wound-healing research done rodents vitro, low rate a significant impediment to will have any impact.To generate clinically relevant experimental data, hypothesis generation should begin, or...
Increasing evidence indicates that the tumor microenvironment plays a critical role in regulating biologic behavior of breast cancer. In veterinary oncology, there is need for improved prognostic markers to accurately identify dogs at risk local and distant (metastatic) recurrence mammary gland carcinoma therefore would benefit from adjuvant therapy. Collagen density fiber organization have been shown regulate progression both mouse human tumors, with certain collagen signatures predicting...
Abstract Bone morphogenetic proteins (BMPs) were originally identified by their ability to induce ectopic bone formation and have been shown promote both chondrogenesis chondrocyte hypertrophy. BMPs recently found activate a membrane serine/threonine kinase signaling mechanism in variety of cell types, but the downstream effectors BMP differentiation remain unidentified. We previously reported that BMP-2 markedly stimulates type X collagen expression prehypertrophic chick sternal...
Clinical trials utilizing bone marrow-derived mesenchymal stem cell (BM-MSC) therapies show promise for treating a variety of pathologic conditions. Paramount to optimization such cell-based is thorough understanding MSC biology. Despite the tremendous potential that exists clinical use canine BM-MSCs in veterinary medicine, as well preclinical studies human relatively little information regarding basic biological properties cells. In this study, we compared importance donor characteristics...
In the murine model, in utero hematopoietic cell transplantation (IUHCT) has been shown to achieve low levels of allogeneic chimerism and associated donor-specific tolerance permitting minimal conditioning postnatal stem (HSCT). this pilot study, we investigated IUHCT canine leukocyte adhesion deficiency (CLAD) model. Haploidentical resulted stable low-level donor all dogs that could be analyzed by sensitive detection methodology (4 10) through 18 months follow-up. 2 CLAD recipients,...