Changgen Shi

ORCID: 0000-0003-3995-3311
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Research Areas
  • Protein Interaction Studies and Fluorescence Analysis
  • Drug Transport and Resistance Mechanisms
  • Amyloidosis: Diagnosis, Treatment, Outcomes

Shanghai Institute of Planned Parenthood Research
2021

Fudan University
2021

ObjectiveThe aim of the present study is to develop and validate a reliable simple application for genotyping serum amyloid A1 (SAA1).MethodsThe specific nested PCR was performed amplify product SAA1 gene. Two quenching probes (QPs) were designed detecting two single nucleotide polymorphism (SNP) sites, rs1136743(C/T) rs1136747(C/T) respectively genotypes. The QPs genotying introduced into fully automated system (I-densy, ARKRAY, Inc.), which enables from whole blood.ResultsSix genotypes...

10.1016/j.heliyon.2021.e06858 article EN cc-by-nc-nd Heliyon 2021-04-01
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