A5100456432- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Machine Learning in Bioinformatics
- Protein Structure and Dynamics
- Genetics, Bioinformatics, and Biomedical Research
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Endoplasmic Reticulum Stress and Disease
- Advanced Biosensing Techniques and Applications
- Protein Interaction Studies and Fluorescence Analysis
- Speech and Audio Processing
- Glycosylation and Glycoproteins Research
- Peptidase Inhibition and Analysis
- Molecular Biology Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Indoor and Outdoor Localization Technologies
- Metabolism and Genetic Disorders
- RNA modifications and cancer
- Metabolomics and Mass Spectrometry Studies
- Genomics and Phylogenetic Studies
- Gene expression and cancer classification
- Drug Transport and Resistance Mechanisms
- Bioinformatics and Genomic Networks
- Wireless Networks and Protocols
- Oral microbiology and periodontitis research
The Ohio State University
2019
Digital Proteomics (United States)
2016
Shanghai Jiao Tong University
2009-2015
Tsinghua University
2007-2013
Shanghai Institutes for Biological Sciences
2013
Chinese Academy of Sciences
2013
The University of Texas at Austin
2012
University of Minnesota
2005-2009
Twin Cities Orthopedics
2009
To assess the potential of tumor-associated, alternatively spliced gene products as a source biomarkers in biological fluids, we have analyzed large data set mass spectra derived from plasma proteome mouse model human pancreatic ductal adenocarcinoma. MS/MS were interrogated for novel splice isoforms using nonredundant database containing an exhaustive three-frame translation Ensembl transcripts and models ECgene. This integrated analysis identified 420 distinct isoforms, which 92 did not...
Eukaryotic elongation factor 2 kinase (eEF-2K) is an atypical protein regulated by Ca2+ and calmodulin (CaM). Its only known substrate eukaryotic (eEF-2), whose phosphorylation eEF-2K impedes global synthesis. To date, the mechanism of autophosphorylation has not been fully elucidated. investigate autophosphorylation, human was coexpressed with λ-phosphatase purified from bacteria in a three-step protocol using CaM affinity column. Purified induced to autophosphorylate incubation Ca2+/CaM...
Abstract Carefully controlled ZipTip extraction of diluted human plasma or serum was combined with MALDI‐TOF‐MS to produce highly reproducible protein profiles. Components detected included apolipoproteins CI, CII and CIII as well transthyretin several isoforms each that are created by glycosylation other modification proteolytic processing. Profiles healthy individuals all contained the same 15 components. Others were found in from disease. analyzed peak ratios within spectrum....
Serum proteomics provides a useful tool to identify potential biomarkers associated with cancer progression. In the present study, two-dimensional liquid chromatography-tandem mass spectrometry (2D-LC-MS/MS) on linear ion trap was utilized and compare serum proteins from breast patients. Three groups of 21 human sera, 7 patients lymph node-negative invasive ductal carcinoma (IDCB), node-positive IDCB, controls benign diseases, were analyzed. Through proteomic analysis, total 2,078 identified...
Abstract Background Proteomic measurements, which closely reflect phenotypes, provide insights into gene expression regulations and mechanisms underlying altered phenotypes. Further, integration of data on proteome transcriptome levels can validate signatures associated with a phenotype. However, proteomic is not as abundant genomic data, it thus beneficial to use features predict protein abundances when matching samples or measurements within are lacking. Results We evaluate compare four...
There has been significant development in indoor positioning techniques based on location fingerprinting wireless networks. AP placement an impact fingerprint precision. In this study, a max-min RSS distance method to optimize KNN is proposed for accuracy and the simulation results are presented IEEE 802.11 TGn channel models.
Abstract Although numerous protein biomarkers have been correlated with advanced disease states, no new clinical assays developed. Goals often anticipate disease‐specific changes that exceed values among healthy individuals, a property common to acute phase reactants. This review considers somewhat different approaches. It focuses on intact isoform ratios present biomarker without change in the total concentration of protein. These will seldom be detected by peptide level analysis or most...
Background. The use of biopsies to determine kidney health after transplantation is an invasive procedure with some risk the patient. Consequently, a noninvasive test for transplanted would provide significant advantage over current clinical practice. Methods. Urines from donors before nephrectomy, pretransplant patients native disease, and posttransplant recipients were examined protein biomarkers diagnose or prognose disease. Proteins extracted by C4 reverse phase affinity analyzed matrix...
Each phase of eukaryotic cell cycle is tightly controlled by multicomponent regulatory networks based on complex relationships protein phosphorylation. In order to better understand the between kinases and their substrate proteins during progression cycle, we analyzed phosphoproteome HeLa cells G1, S, G2/M phases using our developed quantitative phosphoproteomic approaches. A total 4776 high-confidence phosphorylation sites (phosphosites) in 1177 were identified. Bioinformatics analysis for...
As an indispensable part of the Protein Modification Profiling effort in Chinese Human Liver Proteome Project (CNHLPP), a protein modification data integrating and flow processing system named electronic Information System (eMIS) has been established. The eMIS comprises comprehensive database Moddb, backend pipeline, frontend display search web interface. Moddb currently contains nearly 13000 entries modifications from phosphorylation, glycosylation, ubiquitinylation, etc. It is based on...
ObjectiveThe aim of the present study is to develop and validate a reliable simple application for genotyping serum amyloid A1 (SAA1).MethodsThe specific nested PCR was performed amplify product SAA1 gene. Two quenching probes (QPs) were designed detecting two single nucleotide polymorphism (SNP) sites, rs1136743(C/T) rs1136747(C/T) respectively genotypes. The QPs genotying introduced into fully automated system (I-densy, ARKRAY, Inc.), which enables from whole blood.ResultsSix genotypes...