A5100694701- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Molecular Sensors and Ion Detection
- Nanoplatforms for cancer theranostics
- Genomics and Phylogenetic Studies
- Luminescence and Fluorescent Materials
- Immune Cell Function and Interaction
- RNA and protein synthesis mechanisms
- Thermochemical Biomass Conversion Processes
- T-cell and B-cell Immunology
- Biosensors and Analytical Detection
- Analytical Chemistry and Sensors
- MicroRNA in disease regulation
- Molecular Junctions and Nanostructures
- Genomics and Chromatin Dynamics
- Analytical chemistry methods development
- Gene expression and cancer classification
- Sulfur Compounds in Biology
- Lignin and Wood Chemistry
- Hematopoietic Stem Cell Transplantation
- Machine Learning in Bioinformatics
- Bacteriophages and microbial interactions
- Nanocluster Synthesis and Applications
- interferon and immune responses
Beijing National Laboratory for Molecular Sciences
2016-2025
University of Chinese Academy of Sciences
2017-2025
Chinese Academy of Sciences
2015-2024
Second Xiangya Hospital of Central South University
2023-2024
Central South University
2023-2024
Anhui Normal University
2022-2024
Annoroad Gene Technology (China)
2024
Nanjing University of Posts and Telecommunications
2024
Xi'an Jiaotong University
2022-2024
Changsha University of Science and Technology
2023
A simple and effective strategy for designing ratiometric fluorescent nanosensor has been described in this work. carbon dots (CDs) based dual-emission Cu(2+) detection was prepared by coating CDs on the surface of Rhodamine B-doped silica nanoparticles. The were synthesized using N-(β-aminoethyl)-γ-aminopropyl methyldimethoxysilane (AEAPMS) as main raw material, so that residual ethylenediamine groups methoxysilane can serve recognition sites silylation reaction groups. obtained showed...
Mitophagy is a process in which cells remove dysfunctional mitochondria and recycle their constituents lysosome-dependent manner. To probe this process, two different fluorescent dyes specific for lysosomes, respectively, are often used combination. However, current lysosomes cannot distinguish mitochondria-containing autolysosomes from other lysosomes. Therefore, we herein report cyanine dye, HQO, can simultaneously live by exhibiting fluorescence properties. HQO selectively accumulates but...
It has been regarded as an essential treatment option for diabetic nephropathy (DN) in Traditional Chinese medicine. Previous studies have demonstrated the anti-DN efficacy of Schisandra chinensis Fruit Mixture (SM); however, a comprehensive chemical fingerprint is still uncertain, and its mechanism action, especially potential therapeutic targets anti-DN, needs to be further elucidated. Potential mechanisms SM action on DN were explored through network pharmacology experimental validation....
The high degree of similarity between the mouse and human genomes is demonstrated through analysis sequence chromosome 16 (Mmu 16), which was obtained as part a whole-genome shotgun assembly genome. genome about 10% smaller than genome, owing to lower repetitive DNA content. Comparison structure protein-coding potential Mmu with that homologous segments identifies regions conserved synteny chromosomes (Hsa) 3, 8, 12, 16, 21, 22. Gene content order are highly syntenic blocks Of 731 predicted...
DNA sequences with repetitive G-rich structural motifs, which form special structures called G-quadruplexes, widely exist in the human genome. Here we report general peroxidase activity of G-quadruplex-hemin complexes and discuss connection between G-quadruplex structures. The high hemin complexed intramolecular parallel G-quadruplex-forming gene promoters (such as c-Myc, VEGF, c-Kit21, HIF-1alpha, RET) may imply a potential mechanism hemin-mediated cellular injury. This has also been...
In this paper, we describe a turn-on fluorescent probe for Zn(2+) and Cd(2+) ion detection discrimination. This probe, N'-bis-(N,N-di-(2-pyridylmethyl)-ethane-1,2-diamine)-perylene-3,4,9,10-tetracarboxylic-diimide (PDI-DIDPA), exhibits very low fluorescence at pH above 6.0 due to the photo-induced electron transfer (PET) process from 2-pyridylmethyl-amine (DPA) perylene tetracarboxylic diimide (PDI). can chelate PDI-DIDPA form stable complex 6.0-7.0 9.0 respectively, inhibit PET process,...
Random flap grafting is a routine procedure used in plastic and reconstructive surgery to repair reconstruct large tissue defects. Flap necrosis primarily caused by ischemia–reperfusion injury inadequate blood supply the distal flap. Ischemia–reperfusion leads production of excessive reactive oxygen species, creating pathological microenvironment that impairs cellular function angiogenesis. In this study, we developed remodeling self-healing hydrogel [laminarin–chitosan-based hydrogel-loaded...
The superparamagnetic surface molecularly imprinted Fe3O4@MIP nanoparticles for bisphenol A (BPA) were prepared via initiated atom transfer radical polymerization (si-ATRP). Fe3O4 core was compactly encapsulated with a polychloromethylstyrene (PCMS) layer viamini-emulsion polymerization. exhibited property saturation magnetization of 25.2 emu g−1. BPA revealed specific selectivity and high affinity to the template over structural analogues. Moreover, surface-imprinted MIP showed good site...
Putative G-quadruplex-forming sequences (PQS) are highly prevalent in human genome; however, the structures and functions of most PQSs genome poorly understood. Therefore, selective recognition certain types G-quadruplexes (G4s) is important for study G4s. A new light up fluorescent probe, BPBC composed benzimidazole carbazole moieties was designed synthesized. possesses a crescent-shaped π-conjugated planar core that slightly larger than dimension G-quartet plane This structure endows with...
DNA sequences that can form G-quadruplexes (G4s) are highly prevalent in the genome. However, structures and functions of most G4-forming genome poorly understood. Therefore, development molecular probes for G4 recognition biological samples, especially with long wavelength, important basic research G4s. Squaraines dyes exhibit sharp intense absorption strong emission red to NIR region, but very few them have been reported as nucleic acids. Here we report interactions two squaraine dyes, STS...
Allogeneic hematopoietic stem cell transplantation is a widely used and effective therapy for malignant diseases numerous other disorders. High-resolution human leukocyte antigen (HLA) haplotype frequency distributions not only facilitate individual donor searches but also determine the probability with which particular patient can find HLA-matched donors in registry. The frequencies of HLA-A, -B, -C, -DRB1, -DQB1 alleles haplotypes were estimated among 169,995 Chinese volunteers using...
Intracellular and extracellular pH plays a key role in many cell biological processes. Probes for simultaneously monitoring the change inside outside living cells are rarely available. In this paper, we describe new ratiometric fluorophore that was synthesized by condensation of 4-bromine-1,8-naphthalimides 3-amino-1,2,4-triazole. range 5-8, only N-H heterocycle-fused aromatic ring system undergoes reversible deprotonation-protonation process, which results large red shift absorption...
Abstract G‐quadruplex (G4)/hemin DNAzymes have been extensively applied in bioanalysis and molecular devices. However, their catalytic activity is still much lower than that of proteinous enzymes. The G4/hemin DNAzyme correlated with the G4 conformations solution conditions. little known about effect flanking sequences on activity, though they are important parts G4s. Here, we report containing d(CCC), flanked both ends G4‐core remarkably enhance activity. By using circular dichroism...
Lycium barbarum L. is a widely used functional food and medicinal herb in Asian countries. barbarium polysaccharides (LBP) are considered as one of the major components fruit exhibits wide range biological activities. Here, we investigated immunomodulatory effects LBP its uptake behaviors at cellular level. was prepared by water extraction ethanol precipitation, divided into two fractions based on molecular weight distribution ultrafiltration (LBP > 10 kDa < kDa). The physicochemical...
As construction becomes greener, people have higher and requirements for engineering project management, which makes it necessary to deeply study the comprehensive optimization of schedule, cost sustainability level. Adhering concept low carbon green, article takes emission factor into total building improves traditional objective projects; then quantitatively analyzes economic, environmental social impacts assembled buildings from perspective sustainability, introduces duration-cost problem...
Hydrogen peroxide (H(2)O(2)) is the major reactive oxygen species (ROS) produced in sperm. High concentrations of H(2)O(2) sperm induce nuclear DNA fragmentation and lipid peroxidation result cell death. The respiratory chain mitochondrion one most productive ROS generating systems sperm, thus destruction mitochondria critical for cell. It was recently reported that generated by can be efficiently destroyed cytochrome c-mediated electron-leak pathway where electron ferrocytochrome c migrates...
Biogenic polyamines participate in regulating gene expression, activating DNA synthesis and facilitating DNA–protein interaction through with DNA/RNA. The of G-quadruplexes (G4s) has been reported to modulate the structure G4s. In this paper, we investigate effects on one properties G4s, G4/hemin peroxidase. Three (spermine, spermidine putrescine) are found have positive different DNAzymes, which spermine exhibits strongest enhancement efficiency. CD UV/Vis spectral analysis suggests two...
A new carbon dot (CD)-based nanoprobe for the ratiometric fluorescence detection of DNA was constructed in this work. Thiazole orange (TO), a specific organic small molecular probe toward DNA, is covalently linked to surface CDs, acting as recognition element and response unit. In absence only emitted blue whereas TO almost nonfluorescent. Upon addition turn-on emission at 530 nm appeared gradually enhanced along with increasing target CDs unchanged, which realized DNA. The CD–TO showed good...