A5100643823- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- HIV Research and Treatment
- Immunodeficiency and Autoimmune Disorders
- Cytokine Signaling Pathways and Interactions
- Blood groups and transfusion
- Diabetes and associated disorders
- Immune Response and Inflammation
- Cytomegalovirus and herpesvirus research
- Adipokines, Inflammation, and Metabolic Diseases
- HIV/AIDS drug development and treatment
- CAR-T cell therapy research
- Galectins and Cancer Biology
- Chronic Lymphocytic Leukemia Research
- Lymphoma Diagnosis and Treatment
- Systemic Lupus Erythematosus Research
- Asthma and respiratory diseases
- Erythrocyte Function and Pathophysiology
- Cell Adhesion Molecules Research
- Advanced Proteomics Techniques and Applications
- Birth, Development, and Health
- Platelet Disorders and Treatments
- NF-κB Signaling Pathways
Chongqing University of Arts and Sciences
2025
Beijing University of Chinese Medicine
2019-2024
Changzhou Third People's Hospital
2013-2024
Nanjing Medical University
2024
University of Electronic Science and Technology of China
2020-2023
China-Japan Friendship Hospital
2011-2023
Affiliated Hospital of Jining Medical University
2022-2023
Yunnan University
2023
First Affiliated Hospital of Zhengzhou University
2022
Zhengzhou University
2022
Sequence analysis of immunoglobulin (Ig) heavy and light chain transcripts can refine categorization B cell subpopulations shed on the selective forces that act during immune responses or dysregulation, such as autoimmunity, allergy, malignancy. High-throughput sequencing yields Ig transcript collections unprecedented size. The authoritative web-based IMGT/HighV-QUEST program is capable analyzing large provides annotated output files to describe many key properties transcripts. However,...
IgA nephropathy (IgAN) is characterized by circulating immune complexes composed of galactose-deficient IgA1 and a glycan-specific IgG antibody. These deposit in the glomerular mesangium induce mesangioproliferative glomerulonephritis characteristic IgAN. To define precise specificities molecular properties antibodies, we generated EBV-immortalized IgG-secreting lymphocytes from patients with IgAN found that secreted formed glycan-dependent manner. We cloned sequenced heavy- light-chain...
Interleukin-6 (IL-6) and glucocorticoids are important mediators of inflammatory immunological responses. Glucocorticoids known to synergistically enhance IL-6-mediated cellular We now show that IL-6 also has a synergistic effect upon glucocorticoid signaling. In particular, IL-6-activated STAT3 associates with ligand-bound receptor form transactivating/signaling complex, which can function through either an IL-6-responsive element or glucocorticoid-responsive element. These findings reveal...
Systemic autoimmune diseases are a broad range of related characterized by dysregulation immune system which give rise to activation cells attack autoantigens and resulted in inappropriate inflammation multitissue damages. They fascinating but poorly understood group diseases, ranging from the commonly seen rheumatoid arthritis (RA) systemic lupus erythematosus (SLE) relatively rare sclerosis [1]. The mechanism pathogenesis is still not very clear. It now considered that genetic factors,...
Rheumatoid arthritis (RA) is a complex autoimmune disease affecting 1-2% of general worldwide population. The etiopathogenesis RA involves the interplay multiple genetic risk factors and environmental triggers. Microbial infections are believed to play an important role in initiation perpetuation RA. Recent clinical studies have shown association microbial with Accumulated using animal models also found that can induce and/or exaggerate symptoms experimental arthritis. In this review, we...
Abstract The 2019 coronavirus disease (COVID-19) outbreak caused by the SARS-CoV-2 virus is an ongoing global health emergency. However, virus’ pathogenesis remains unclear, and there no cure for disease. We investigated dynamic changes of blood immune response in patients with COVID-19 at different stages using 5’ gene expression, T cell receptor (TCR), B receptors (BCR) V(D)J transcriptome analysis a single-cell resolution. obtained mRNA sequencing (scRNA-seq) data 341,420 peripheral...
Allogeneic hematopoietic stem cell transplantation is a widely used and effective therapy for malignant diseases numerous other disorders. High-resolution human leukocyte antigen (HLA) haplotype frequency distributions not only facilitate individual donor searches but also determine the probability with which particular patient can find HLA-matched donors in registry. The frequencies of HLA-A, -B, -C, -DRB1, -DQB1 alleles haplotypes were estimated among 169,995 Chinese volunteers using...
Fibrinogen, a hepatically derived class II acute phase protein, is the product of three separate genes, (Aα, Bβ, and γ). The fibrinogen genes are expressed constitutively; however, their transcription can be significantly up-regulated by interleukin-6 (IL-6) glucocorticoid. Inspection promoter region γ gene revealed hexanucleotide clusters CTGGGA that recognized as IL-6 responsive elements. Functional analyses these regions (designated here site I, II, III according to position in promoter)...
Long-lived memory B cells (B Mem ) provide an archive of historic Ab responses. By contrast, circulating Abs typically decline once the immunogen is cleared. Consequently, can underestimate nature cognate humoral immunity. On other hand, pool should a comprehensive picture specificities that arise over entire course infection. To test this hypothesis, we compared and from natural virus suppressors who control HIV-1 without therapy maintain relatively intact immune system. We found high...
Anti-citrullinated protein antibodies (ACPAs) are highly specific for rheumatoid arthritis (RA). However, the molecular basis ACPA production is still unclear. The purpose of this study was to determine if circulating plasmablasts from RA patients produce ACPAs and whether Porphyromonas gingivalis facilitates generation ACPAs.Using a single-cell antibody cloning approach, we generated 217 110 monoclonal recombinant 7 4 healthy controls, respectively. Antibody reactivity with citrullinated...
Abstract Background Cancer stem cells (CSCs) are regarded as the "seed cells" for tumorigenesis, metastasis, recurrence and drug resistance. However, specific surface markers of CSCs different origins have not been documented. Methods Single-cell sequencing was used to analyze highly expressed genes in cancer gastric patients, it verified that AQP5 specifically (GC-CSCs) vivo vitro. The effect AQP5-promoting LGR5 on malignant biological function GC-CSCs investigated. mechanism by which...
Cancer stem cells (CSCs) are considered tumor-initiating and the main drivers of disease progression. Targeting these rare cancer cells, however, remains challenging with respect to therapeutic benefit. Here, we report up-regulation IL-13RA2 expression in colorectal (CRC) tissues spheroid cells. The was positively correlated canonical stemness markers CRC. We further demonstrated that level IL-13 up-regulated serum CRC patients. Biologically, recombinant (rIL-13) stimulation promoted sphere...
Initiation of reverse transcription human immunodeficiency virus type 1 (HIV-1) occurs by extension from the 3' end a cellular tRNA complexed to primer binding site (PBS) located near 5' viral RNA genome. Although PBSs for all naturally occurring HIV-1 viruses are complementary 3'-terminal 18 nucleotides tRNA(Lys)3, we identified an which contained PBS tRNA(Met); this was not stable upon extended culture and reverted back wild (S.-M. Kang, J. K. Wakefield, C. D. Morrow, Virology 222:401-414,...
Here we present a novel and robust method for the identification of protein S-nitrosylation sites in complex mixtures. The approach utilizes cysteinyl affinity resin to selectively enrich S-nitrosylated peptides reduced by ascorbate followed nanoscale liquid chromatography tandem mass spectrometry. Two alkylation agents with different added masses were employed differentiate from non-S-nitrosylation sites. We applied this MDA-MB-231 cells treated Angeli's salt, nitric oxide donor that has...
The first wave of the 2019 novel coronavirus (COVID-19) epidemic in China showed there was a lag between reduction human mobility and decline COVID-19 transmission this different cities. A prolonged would cause public panic reflect inefficiency control measures. This study aims to quantify time-lag effect reveal its influencing socio-demographic environmental factors, which is helpful policymaking controlling other potential infectious diseases future. We combined city-level index new case...
Current therapeutic strategies for Alzheimer's disease (AD) mainly focus on inhibition of aberrant amyloid-β peptide (Aβ) aggregation. However, these cannot repair the side symptoms (e.g., high neuronal oxidative stress) triggered by Aβ accumulation and thus show limited effects suppressing Aβ-induced apoptosis. Herein, we develop a stepwise metal-phenolic coordination approach rational design neuroprotection enhancer, K8@Fe-Rh/Pda NPs, in which rhein polydopamine are effectively coupled to...