Abstract Study Objectives There is strong evidence that sleep disturbances are an independent risk factor for the development of chronic pain conditions. The mechanisms underlying this association, however, still not well understood. We examined effect experimental (ESDs) on three pathways involved in initiation/resolution: (1) central pain-inhibitory pathway, (2) cyclooxygenase (COX) and (3) endocannabinoid (eCB) pathway. Methods Twenty-four healthy participants (50% females) underwent two...

Abstract Sleep disturbances, including disrupted sleep and short duration, are highly prevalent prospectively associated with an increased risk for various widespread diseases, cardiometabolic, neurodegenerative, chronic pain, autoimmune diseases. Systemic inflammation, which has been observed in populations experiencing may mechanistically link disturbed disease risks. To determine whether disturbances causally responsible the inflammatory changes reported population-based studies, we...

ABSTRACT Introduction Insomnia is a highly prevalent condition that predisposes individuals to many chronic pain disorders, with most of them showing pronounced sexual dimorphism. We investigated whether experimental insomnia‐like sleep disturbances (ESD) affect spontaneous and sensitivity, sex modulates responses. Methods Twenty‐four healthy participants (50% females, age 28.3 ± 5.9 years) participated in study consisting two 19‐day in‐laboratory protocols—an ESD protocol repeated nights...

Abstract Background Environmental factors, notably changes in diet and microbiota composition, have been identified as key contributors to the development of inflammatory bowel diseases (IBD). The prevalence IBD is on rise, particularly industrialized nations with populations consuming western-style diets rich fat protein. While total animal protein intake associated IBD, their specific impact intestinal inflammation remains poorly understood. Branch-chain amino acids found different source...

Abstract Background Patients with inflammatory bowel disease (IBD) report diverse food-related adverse reactions. Dairy is the most self-reported food intolerance in IBD. Emerging evidence suggests that altered diet-microbiota interactions can contribute to development of IBD and reaction foods. We hypothesize microbial alterations patients facilitate reactions Aims To investigate role intestinal inflammation on metabolism sensitization. Methods effects food, eight-week-old C57BL6 mice were...

Abstract Study Objectives Persistent post-acute sequelae of SARS-CoV-2 infection, i.e., long COVID, impacts multiple organ systems. While lower blood oxygen is expected when infects the lungs, hypoxia without pulmonary symptoms may continue after acute phase. Ventilation and are more vulnerable during sleep, but nocturnal hypoxemia hasn’t been studied in people with COVID a facility setting using gold-standard polysomnography (PSG). Methods We conducted an observational study 50 participants...

Abstract Introduction Persistent post-acute sequelae of SARS-CoV-2, i.e., long COVID, is a heterogeneous syndrome involving multiple organ systems. While lower blood oxygen expected when SARS-CoV-2 infects the lungs, hypoxia without pulmonary symptoms can continue after acute phase. Ventilation and are more vulnerable during sleep, but nocturnal hypoxemia has not been studied in COVID facility setting using polysomnography (PSG). Here, we characterized levels sleep. Methods This was an...

While it is well established that slow-wave sleep electroencephalography (EEG) rebounds following deprivation, very little research has investigated autonomic nervous system recovery. We examined heart rate variability (HRV) and cardiovagal baroreflex sensitivity (BRS) during four blocks of repetitive restriction sequential nights recovery sleep. Twenty-one healthy participants completed the 22-day in-hospital protocol. Following three 8-hr sleep, they were assigned to a condition....

Abstract Study Objective To evaluate how nocturnal timing of sleep restriction affects vigilant attention and mood in healthy controls with normal sleep–wake patterns. Methods A convenience sample from two controlled protocols were used to investigate the difference between 4 hours early night, versus late night. Volunteers stayed a hospital setting randomized one three conditions: control (8 each night), an short (ESS, 2300–0300 hours), (LSS, 0300–0700 hours). Participants evaluated...

Abstract Introduction Sleep disturbances deteriorate immune function by not only affecting pro-inflammatory pathways, but also inflammatory resolution which actively terminate inflammation. It is assumed that slow wave sleep (SWS) amount and activity (SWA) convey the immune-supportive functions of sleep. We investigated whether changes in SWS induced experimental disturbance followed recovery predict mediators. Methods The randomized controlled within-subjects trial (N=24, 20-42 years, 12...

Abstract Background Intestinal microbiota, diet, and the immune system have been proposed to contribute development of inflammatory bowel diseases (IBD). The aryl hydrocarbon receptor (AhR) is a critical regulator intestinal immunity mucosal barrier homeostasis that activated by agonists such as host microbial tryptophan metabolites. IBD patients altered microbiota reduced AhR in content resulting downregulation AhR. Our recent findings indicate mice harbouring with impaired metabolism...

Abstract Background Patients with chronic intestinal conditions, including inflammatory bowel disease (IBD), experience diverse food-related adverse reactions. However,, the precise mechanisms of food intolerances in IBD remains unclear. Emerging evidence suggests that altered diet-microbiota interactions can contribute to development IBD. Previously we shown microbiota plays an important role intolerances. We hypothesize microbial alterations patients facilitate reactions foods. Aims To...

Abstract Introduction Sleep deficiencies, such as manifested in short sleep duration or insomnia symptoms, are known to increase the risk for multiple chronic diseases. Inflammation is considered a mechanism through which deficient acts factor these Thus, mitigating inflammation might be potential way diminish negative health consequences related deficiency. To investigate pharmacological approach this, we used low-dose acetylsalicylic acid (ASA, aspirin), its counter-inflammatory actions...

Abstract Introduction Fatigue and pain are prominent features of functional impairment in insomnia. This study aimed to better understand behavioral physiological mechanisms these complex relationships. Methods 22 participants with insomnia disorder (DSM-5 criteria, 18 female, age 18-49yrs) good-sleeper controls (19 18-47yrs) completed two-weeks sleep logs actigraphy recordings prior coming the laboratory for overnight polysomnography subsequent daytime testing that included questionnaires,...

Abstract Introduction Sleep disturbances are more common in women than men, as many chronic pain disorders characterized by inflammation and fatigue. This study investigated sex differences fatigue responses to sleep disruption whether such recover with uninterrupted sleep. Methods 24 healthy young individuals (12 women; ages 18–42 yrs) participated a consisting of two counterbalanced 19-day experimental in-hospital stays, separated months. Following 3 baseline nights, participants were...

Abstract Introduction Chronic low-grade systemic inflammation is involved in the pathogenesis of many human diseases. Common sleep patterns restricting during weekdays and catching up on over weekend induce inflammatory upregulation that may not resolve following recovery sleep. We hypothesize this pattern leads to an imbalance markers regulating homeostasis, including (eg, interleukin-6 (IL-6) cyclooxygenase 2 (COX-2)) counter-inflammation glucocorticoids (GCs)). The enzyme COX-2...

Abstract Introduction Insufficient sleep has been shown to increase the risk of a person developing hypertension. Impaired baroreflex sensitivity (BRS) is one known underlying mechanisms involved that responsible for increasing blood pressure (BP). This project investigates relationship between sleep, BRS, and BP during Valsalva’s Maneuver (VM). Methods Fifty participants (59.8 ± 1.5 years; 31 women) completed 3 overnight in-hospital stays. The first stay (S1) was baseline control; second...

Introduction: Insufficient sleep is associated with increased heart rate (HR) and blood pressure (BP). Exaggerated cardiovascular reactivity to the cold pressor test (CPT) has been linked an incidence of hypertension. This project investigates effects improved hygiene on autonomic function at rest in response CPT. We hypothesize a decrease BP HR rest, attenuated during Methods: Fifty participants (59.8 ± 1.5 years; 31 women) completed 3 overnight in-hospital stays which they had continuous...