A5100713494- Organ Transplantation Techniques and Outcomes
- Cancer Mechanisms and Therapy
- Drug Transport and Resistance Mechanisms
- Trace Elements in Health
- Immune Cell Function and Interaction
- Transplantation: Methods and Outcomes
- Cancer Cells and Metastasis
- MicroRNA in disease regulation
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Pancreatic and Hepatic Oncology Research
- Renal Transplantation Outcomes and Treatments
- DNA Repair Mechanisms
- Epigenetics and DNA Methylation
- Molecular Biology Techniques and Applications
- Medicinal Plant Pharmacodynamics Research
- Heavy Metal Exposure and Toxicity
- Cancer-related Molecular Pathways
- Reproductive System and Pregnancy
- Cell death mechanisms and regulation
- Radiopharmaceutical Chemistry and Applications
- Ginkgo biloba and Cashew Applications
- Traditional Chinese Medicine Analysis
- Phytochemical compounds biological activities
- Cancer Immunotherapy and Biomarkers
- Microtubule and mitosis dynamics
Zhujiang Hospital
2012-2024
Southern Medical University
2012-2024
The People's Hospital of Guangxi Zhuang Autonomous Region
2024
City of Hope
2019-2023
Zhongshan Hospital
2020-2021
Fudan University
2020-2021
The First Affiliated Hospital, Sun Yat-sen University
2020-2021
Sun Yat-sen University
2020-2021
Union Hospital
2021
Huazhong University of Science and Technology
2021
Zinc is an essential trace element and catalytic/structural component used by many metalloenzymes transcription factors. Recent studies indicate a possible correlation of zinc levels with the cancer risk; however, exact role transporters in progression unknown. We have observed that transporter, ZIP4 (SLC39A4), was substantially overexpressed 16 17 (94%) clinical pancreatic adenocarcinoma specimens compared surrounding normal tissues, mRNA expression significantly higher human cells than...
Abstract Purpose: C-X-C chemokine receptor type 2 (CXCR2) is a key regulator that drives immune suppression and inflammation in tumor microenvironment. CXCR2-targeted therapy has shown promising results several solid tumors. However, the underlying mechanism of CXCR2-mediated cross-talk between gastric cancer cells macrophages still remains unclear. Experimental Design: The expression CXCR2 its ligands 155 human tissues was analyzed via immunohistochemistry, correlations with clinical...
Abstract Purpose: The most common genetic lesions in pancreatic ductal adenocarcinoma (PDAC) have been identified. However, significant gaps still exist our understanding of how such alterations act concert to induce PDAC development. In this study, we investigated the mechanism tumorigenic transformation immortalized human epithelial (HPDE) cell line by sequentially introducing signature into line. Experimental Design: phenotype for stable expression mutant K-ras, Her2, p16/p14shRNA, and...
Emerging evidence implicates the zinc importer ZIP4 as a critical factor that enhances pancreatic cancer proliferation; however, role of in promoting progression by regulating apoptosis requires elucidation. To determine effect on apoptosis, we used cell lines where levels were upregulated or silenced combination with Chelex 100 treatment to deplete intracellular zinc. Pancreatic xenografts derived from those cells also included. TUNEL and flow cytometry analysis measure western blotting was...
Cisplatin-based chemotherapy is the first-line treatment for non-small cell lung cancer (NSCLC), but drug resistance occurs in most patients, leading to failure. Recent studies have shown that epithelial–mesenchymal transition (EMT) associated with resistance. However, underlying mechanism not entirely clear. In this study, first we showed significant positive correlation between expression of ERCCl and vimentin, negative E-cadherin neoadjuvant group simple surgery group. Second,...
Background/Aims: Aberrant expression of miRNA has been found in many tumor tissues to regulate the tumorigenesis by binding 3`- untranslated region (3`-UTR) target genes. The aim this study is investigate role miR-148b, miR-152/ALCAM axis human pituitary adenomas (PAs). Methods: First, we detected level miR-148b-3p and miR-152 PAs samples using qRT-PCR. Then studied miR-148b-3p, on cell proliferation, invasion apoptosis MTS assay, Transwell assay Annexin V/PI Staining Test. To relationship...
Kidney transplantation is currently the first choice of treatment for various types end-stage renal failure, but there are major limitations in application immunosuppressive protocols after kidney transplantation. When dose immunosuppressant too low, graft rejection occurs easily, while a that high can lead to loss. Therefore, it very important explore immune status patients receiving agents To compare recipient’s whole peripheral blood before and receipt agents, we used single-cell...
Y box-binding protein 1 (YB-1) is a potent oncogenic protein. How it regulates Snail in most tumors including cervical cancer unknown. This article to study if YB-1 plays role via regulating the expression of Snail.Immunohistochemical staining YB-1, Snail, and E-cadherin (E-cad) was performed on tissue specimens 35 cases chronic cervicitis (as control), intraepithelial neoplasm (CIN) I, CIN II/III, 28 unmetastatic squamous cell carcinoma, 19 metastatic carcinoma. RNA interference technique...
Molecular mechanisms underlying the tumorigenesis of a highly malignant cancer, cholangiocarcinoma (CCA), are still obscure. In our study, CCA expression profile data were acquired from The Cancer Genome Atlas (TCGA) database, and differentially expressed genes (DEGs) in TCGA-Cholangiocarcinoma (TCGA-CHOL) set utilized to construct co-expression network via weighted gene analysis (WGCNA). blue module associated with histopathologic grade was screened. Then, five candidate hub screened by...
Abstract Recent studies have reported that tumor‐infiltrating mast cells (TIM) play an important role in tumor regression, but the effect of TIM gallbladder cancer (GBC) remains unclear. The present study aims to investigate prognostic value GBC patients and its responsiveness gemcitabine‐based adjuvant chemotherapy (ACT). A total 298 from Zhongshan Hospital were recruited for this study. infiltration was measured by immunohistochemical staining. Accumulation is significantly associated with...
Gallbladder cancer (GBC) is a common malignancy of the biliary tract and characterized by rapid progression early metastasis. Elucidating molecular mechanisms GBC could help to develop better treatment strategies.Human cell lines (GBC-SD NOZ) were applied determine capacity proliferation migration cells using MTT assay, colony formation, wound-healing assay as well Transwell™ assay. A nude xenograft was used evaluate tumor growth in vivo.Using two types lines, we found that absence solute...
Utilizing a gene reporter technique to study the effects of Andrographitis Herba on human CXCR4 and CCR5 promoters.Inhibition T cells healthy volunteers was analyzed by RT PCR, Western blot flow cytometry. The promoters driving luciferase in vectors pGLA. 17-CXCR4 17-CCR5 were transfected into H9 stem cells. G418 used for selecting stable cell lines. Rat sera thus medicated collected added cells, which expression detected.They showed that mRNA protein levels CD4+ decreased significantly...
<b><i>Introduction:</i></b> Prolonged exposure to a complete button battery can cause severe tissue necrosis in the eye and permanent impairment of visual function. The main mechanism injury is current generated by hydrolysis fluid at negative electrode production hydroxide ions. <b><i>Case Presentation:</i></b> A 3-year-old girl went local hospital because swelling pain her right 12-h duration. doctor performed an orbital CT (computed...
ABSTRACT Purpose Transcription-replication conflicts (TRCs) are a major source of endogenous replication stress in cancer. We previously discovered that pancreatic ductal adenocarcinoma (PDAC) demonstrates uniquely high levels TRCs compared to other common solid tumors. Here, we characterize the mechanism action, oncogene-dependency, PDAC subtype-specificity, and preclinical activity TRC-targeting small molecule – AOH1996 spectrum models. also provide first clinical evidence patient....
In this research, a magnetophoretic separation device was designed, fabricated and tested using magnetic nanoparticles (MNPs) for more efficiently separating target foodborne pathogens. This consisted of rectangular channel with steel rod on its top, both which were immersed in homogenous field generated by two permanent magnets. A sample containing pathogens incubated biotin conjugated antibody (Ab) then streptavidin coated MNPs diameter 30 nm to form MNPs-Ab-cell complexes, the cell. The...