A5033602704- Malaria Research and Control
- Mosquito-borne diseases and control
- Global Maternal and Child Health
- Genetics, Bioinformatics, and Biomedical Research
- Child Nutrition and Water Access
- Genetic diversity and population structure
- Viral Infections and Vectors
- Biomedical Research and Pathophysiology
- Complement system in diseases
- Enterobacteriaceae and Cronobacter Research
- Community Development and Social Impact
- Neonatal Health and Biochemistry
- Genomics and Phylogenetic Studies
- Molecular Biology Techniques and Applications
- Livestock and Poultry Management
- Antibiotic Resistance in Bacteria
- Computational Drug Discovery Methods
- vaccines and immunoinformatics approaches
- Bacteriophages and microbial interactions
- Salmonella and Campylobacter epidemiology
- Machine Learning in Bioinformatics
- Hemoglobinopathies and Related Disorders
- HIV/AIDS drug development and treatment
- HIV Research and Treatment
- HIV/AIDS Research and Interventions
Manhiça Health Research Centre
2017-2024
Saint Augustine's University
2010
Abstract Routine sampling of pregnant women at first antenatal care (ANC) visits could make Plasmodium falciparum genomic surveillance more cost-efficient and convenient in sub-Saharan Africa. We compare the genetic structure parasite populations sampled from 289 ANC users 93 children community Mozambique between 2015 2019. Samples are amplicon sequenced targeting 165 microhaplotypes 15 drug resistance genes. Metrics diversity relatedness, as well prevalence markers, consistent two...
Abstract Background Targeted next-generation sequencing offers the potential for consistent, deep coverage of information-rich genomic regions to characterize polyclonal Plasmodium falciparum infections. However, methods identify and sequence these are currently limited. Methods A bioinformatic pipeline multiplex were developed simultaneously 100 targets applied dried blood spot (DBS) controls field isolates from Mozambique. For comparison, whole-genome data generated same controls. Results...
Malaria programmes use Plasmodium falciparum histidine-rich protein-2 (PfHRP2) based rapid diagnostic tests (RDTs) for malaria diagnosis. The deletion of this target antigen could potentially lead to misdiagnosis, delayed treatment and continuation active transmission.Plasmodium isolates (n = 1162) collected in Southern Mozambique were assessed by RDTs, microscopy and/or 18SrRNA qPCR. pfhrp2 pfhrp3 deletions investigated from individuals who negative RDT but positive qPCR 69) using...
Background Malaria eradication remains the long-term vision of World Health Organization (WHO). However, whether malaria elimination is feasible in areas stable transmission sub-Saharan Africa with currently available tools a subject debate. This study aimed to evaluate multiphased project interrupt Plasmodium falciparum rural district southern Mozambique. Methods and findings A before-after was conducted between 2015 2018 Magude, 48,448 residents living 10,965 households. Building on an...
Pregnant women attending first antenatal care (ANC) visits represent a promising malaria surveillance target in Sub-Saharan Africa. We assessed the spatio-temporal relationship between trends at ANC (n = 6471) and children community 3933) health facilities 15,467) southern Mozambique (2016-2019). P. falciparum rates detected by quantitative polymerase chain reaction mirrored children, regardless of gravidity HIV status (Pearson correlation coefficient [PCC] > 0.8, χ²<1.1), with 2-3 months...
Imported malaria is a critical obstacle to achieving elimination in low transmission settings. Characterising importation and sources using human mobility parasite genomics has the potential inform strategies, but tools combining both types of data are lacking. We developed novel Bayesian approach that provides individual probabilities geographic origin P. falciparum cases by epidemiological, genetic data. Spatial structure connectivity were assessed microhaplotype-based relatedness...
An ultrasensitive malaria rapid diagnostic test (RDT) was recently developed for the improved detection of low-density Plasmodium falciparum infections. This study aimed to compare performance PfHRP2-based Abbott Malaria Ag P. RDT (uRDT) that conventional SD-Bioline (cRDT) when performed under field conditions.Finger-prick blood samples were collected from adults and children in two cross-sectional surveys May 2017 southern Mozambique. Using real-time quantitative PCR (RT-qPCR) as reference...
Background Mass drug administration (MDA) can rapidly reduce the burden of Plasmodium falciparum (Pf). However, concerns remain about its contribution to select for antimalarial resistance. Methods We used Sanger sequencing and real-time PCR determine proportion molecular markers associated with resistance (k13, pfpm2, pfmdr1 pfcrt) in Pf isolates collected before (n = 99) after 112) implementation two monthly MDA rounds dihydroartemisinin–piperaquine (DHAp) consecutive years Magude district...
Abstract Background Glucose-6-phosphate dehydrogenase (G6PD) deficiency is an X-linked hereditary enzymatic abnormality that affects more than 400 million people worldwide. Most deficient individuals do not manifest any symptoms; however, several precipitant agents—such as fava intake, infections, or drugs—may trigger acute haemolytic anaemia. Countries should be informed of the prevalence this anomaly within their borders, in order to make safe and appropriate national decisions regarding...
Background This study aimed to capture the acceptability prior to, during and after implementation of first year MDA rounds conducted under Magude project, a malaria elimination project in southern Mozambique. Methods was mixed-methods study, consisting focus group discussions (FGDs) (September 2015), non-participant observations (NPOs) (November 2015 –beginning February 2016), semi-structured interviews (SSIs) second round (end 2016). Community leaders, women reproductive age, general...
A significant fraction (46/108, 43%) of swine isolates Campylobacter coli but none 81 C. from turkeys had genomic DNA that was resistant to digestion by MboI, suggesting methylation adenines at GATC sites. No consistent association noted between antimicrobial resistance and MboI resistance. Seven swine-associated multilocus sequence typing-based types (STs) were detected among multiple with MboI-resistant DNA. The data suggest host-associated modification system(s) specific for adenine sites...
Abstract Background Targeted next generation sequencing offers the potential for consistent, deep coverage of information rich genomic regions to characterize polyclonal Plasmodium falciparum infections. However, methods identify and sequence these are currently limited. Methods A bioinformatic pipeline multiplex were developed simultaneously 100 targets applied dried blood spot (DBS) controls field isolates from Mozambique. For comparison, WGS data generated same controls. Results Using...
Abstract Pregnant women attending first antenatal care (ANC) visits represent a promising malaria surveillance target in Sub-Saharan Africa. Here we assessed the spatio-temporal relationship between at ANC (n=6,471), children community(n=9,362) and health facilities (n=15,467) southern Mozambique (2016-2019). P. falciparum rates detected by quantitative polymerase chain reaction mirrored children, regardless of gravidity HIV status (Pearson correlation coefficient [PCC]>0.8, χ²<1.1),...
Abstract Large-scale programs targeting Plasmodium falciparum ( Pf ) elimination can exert strong selection pressures on the parasite population. To better understand impact that initiatives have genetic structure and gametocyte carriage, we applied amplicon-based sequencing of two polymorphic genes quantitative reverse-transcription PCR gametocyte-specific to isolates collected in Magude District (Southern Mozambique) before after an initiative. The 71% reduction prevalence achieved 2 years...
Background: Routine sampling of pregnant women at first antenatal care (ANC) visits could make Plasmodium falciparum genomic surveillance more cost-efficient and convenient in sub-Saharan Africa. We aimed to compare the genetic structure ANC community parasite populations, including changes following an elimination program. Methods: 289 attendees 93 children from were sampled low-to-moderate transmission Maputo province, Mozambique, between 2015 2019. Samples amplicon sequenced targeting 165...
Abstract Routine sampling of pregnant women at first antenatal care (ANC) visits could make Plasmodium falciparum genomic surveillance more cost-efficient and convenient in sub-Saharan Africa. We compared the genetic structure parasite populations sampled from 289 ANC attendees 93 children community Mozambique between 2015 2019. Samples were amplicon sequenced targeting 165 microhaplotypes 15 drug resistance genes. Metrics diversity relatedness, as well prevalence markers, consistent two...
<h3>Background</h3> Malaria Mass Drug Administration (MDA) is recommended to reduce malaria in low transmission settings, with a target coverage of ≥80%. This study assesses the feasibility programmatic MDA (pMDA) pilot implementation southern Mozambique. <h3>Methods</h3> The National Control Programme implemented pMDA Chidenguele (Gaza Province), where estimated population 59,271. Two rounds door-to-door distribution (using satellite maps previously enumerated households (Reveal® platform))...