A5078757932- Malaria Research and Control
- Mosquito-borne diseases and control
- Computational Drug Discovery Methods
- Global Maternal and Child Health
- Parasites and Host Interactions
- Drug-Induced Hepatotoxicity and Protection
- Livestock and Poultry Management
- Genetics, Bioinformatics, and Biomedical Research
- Complement system in diseases
- Hemoglobinopathies and Related Disorders
- Drug Transport and Resistance Mechanisms
- Child Nutrition and Water Access
- Viral Infections and Vectors
- HIV/AIDS drug development and treatment
- Travel-related health issues
- Hepatitis C virus research
Manhiça Health Research Centre
2020-2024
Abstract Routine sampling of pregnant women at first antenatal care (ANC) visits could make Plasmodium falciparum genomic surveillance more cost-efficient and convenient in sub-Saharan Africa. We compare the genetic structure parasite populations sampled from 289 ANC users 93 children community Mozambique between 2015 2019. Samples are amplicon sequenced targeting 165 microhaplotypes 15 drug resistance genes. Metrics diversity relatedness, as well prevalence markers, consistent two...
Abstract Mozambique is one of the four African countries which account for over half all malaria deaths worldwide, yet little known about parasite genetic structure in that country. We performed P. falciparum amplicon and whole genome sequencing on 2251 malaria-infected blood samples collected 2015 2018 seven provinces to genotype antimalarial resistance markers interrogate population using genome-wide microhaplotyes. Here we show only resistance-associated observed at frequencies above 5%...
Pregnant women attending first antenatal care (ANC) visits represent a promising malaria surveillance target in Sub-Saharan Africa. We assessed the spatio-temporal relationship between trends at ANC (n = 6471) and children community 3933) health facilities 15,467) southern Mozambique (2016-2019). P. falciparum rates detected by quantitative polymerase chain reaction mirrored children, regardless of gravidity HIV status (Pearson correlation coefficient [PCC] > 0.8, χ²<1.1), with 2-3 months...
Imported malaria is a critical obstacle to achieving elimination in low transmission settings. Characterising importation and sources using human mobility parasite genomics has the potential inform strategies, but tools combining both types of data are lacking. We developed novel Bayesian approach that provides individual probabilities geographic origin P. falciparum cases by epidemiological, genetic data. Spatial structure connectivity were assessed microhaplotype-based relatedness...
An ultrasensitive malaria rapid diagnostic test (RDT) was recently developed for the improved detection of low-density Plasmodium falciparum infections. This study aimed to compare performance PfHRP2-based Abbott Malaria Ag P. RDT (uRDT) that conventional SD-Bioline (cRDT) when performed under field conditions.Finger-prick blood samples were collected from adults and children in two cross-sectional surveys May 2017 southern Mozambique. Using real-time quantitative PCR (RT-qPCR) as reference...
Background Mass drug administration (MDA) can rapidly reduce the burden of Plasmodium falciparum (Pf). However, concerns remain about its contribution to select for antimalarial resistance. Methods We used Sanger sequencing and real-time PCR determine proportion molecular markers associated with resistance (k13, pfpm2, pfmdr1 pfcrt) in Pf isolates collected before (n = 99) after 112) implementation two monthly MDA rounds dihydroartemisinin–piperaquine (DHAp) consecutive years Magude district...
Introduction Genomic data constitute a valuable adjunct to routine surveillance that can guide programmatic decisions reduce the burden of infectious diseases. However, genomic capacities remain low in Africa. This study aims operationalise functional malaria molecular system Mozambique for guiding control and elimination. Methods analyses prospective seeks generate Plasmodium falciparum genetic (1) monitor markers drug resistance deletions rapid diagnostic test targets; (2) characterise...
Artemisinin-based combination therapy (ACT) has been the recommended first-line treatment for uncomplicated malaria in Mozambique since 2006, with artemether-lumefantrine (AL) and amodiaquine-artesunate (AS-AQ) as first choice. To assess efficacy of currently used ACT, an vivo therapeutic study was conducted.The conducted four sentinel sites: Montepuez, Moatize, Mopeia Massinga. Patients between 6 59 months old Plasmodium falciparum (2000-200,000 parasites/µl) were enrolled February...
Due to the threat of emerging anti-malarial resistance, World Health Organization recommends incorporating surveillance for molecular markers resistance into routine therapeutic efficacy studies (TESs). In 2018, a TES artemether-lumefantrine (AL) and artesunate-amodiaquine (ASAQ) was conducted in Mozambique, prevalence polymorphisms pfk13, pfcrt, pfmdr1 genes associated with drug investigated. Children aged 6-59 months were enrolled four study sites. Blood collected dried on filter paper...
Introduction Malaria molecular surveillance has the potential to generate information on biological threats that compromise effectiveness of antimalarial interventions. This study aims streamline activities inform new strategic plan Mozambican National Control Programme (2023–2030) for malaria control and elimination. Methods analyses prospective genomic Plasmodium falciparum genetic data monitor diagnostic failures due pfhrp2/3 deletions markers drug resistance, characterise transmission...
Abstract Pregnant women attending first antenatal care (ANC) visits represent a promising malaria surveillance target in Sub-Saharan Africa. Here we assessed the spatio-temporal relationship between at ANC (n=6,471), children community(n=9,362) and health facilities (n=15,467) southern Mozambique (2016-2019). P. falciparum rates detected by quantitative polymerase chain reaction mirrored children, regardless of gravidity HIV status (Pearson correlation coefficient [PCC]>0.8, χ²<1.1),...
Article summary This study suggests that: 1) patients given AL infected with parasites carrying N86 were statistically more likely to experience a recurrent infection; 2) ASAQ 86Y infection. Background Plasmodium falciparum multidrug resistance transporter 1 ( Pfmdr1 ) gene mutations are associated altered response artemisinin-based combination therapies (ACTs), particularly those containing the partner drugs lumefantrine and amodiaquine (i.e., artemether-lumefantrine [AL]...
<ns3:p>Introduction Malaria molecular surveillance has the potential to generate information on biological threats that compromise effectiveness of antimalarial interventions. This study aims streamline activities inform new strategic plan Mozambican National Control Program (2023-2030) for malaria control and elimination. Methods analyses prospective genomic <ns3:italic>P. falciparum</ns3:italic> genetic data monitor diagnostic failures due <ns3:italic>pfhrp2/3</ns3:italic> deletions...
Abstract Background : Artemisinin-based combination therapy has been the recommended treatment for uncomplicated malaria in Mozambique since 2006, with artemether-lumefantrine (AL) and amodiaquine-artesunate (AS-AQ) as first-line treatments. To assess efficacy of currently used artemisinin-based therapy, an vivo therapeutic study was conducted. Methods The conducted four sentinel sites: Montepuez, Moatize, Mopeia Massinga. Patients between 6 to 59 months old Plasmodium falciparum...
Abstract Large-scale programs targeting Plasmodium falciparum ( Pf ) elimination can exert strong selection pressures on the parasite population. To better understand impact that initiatives have genetic structure and gametocyte carriage, we applied amplicon-based sequencing of two polymorphic genes quantitative reverse-transcription PCR gametocyte-specific to isolates collected in Magude District (Southern Mozambique) before after an initiative. The 71% reduction prevalence achieved 2 years...
Background: Routine sampling of pregnant women at first antenatal care (ANC) visits could make Plasmodium falciparum genomic surveillance more cost-efficient and convenient in sub-Saharan Africa. We aimed to compare the genetic structure ANC community parasite populations, including changes following an elimination program. Methods: 289 attendees 93 children from were sampled low-to-moderate transmission Maputo province, Mozambique, between 2015 2019. Samples amplicon sequenced targeting 165...
Abstract Routine sampling of pregnant women at first antenatal care (ANC) visits could make Plasmodium falciparum genomic surveillance more cost-efficient and convenient in sub-Saharan Africa. We compared the genetic structure parasite populations sampled from 289 ANC attendees 93 children community Mozambique between 2015 2019. Samples were amplicon sequenced targeting 165 microhaplotypes 15 drug resistance genes. Metrics diversity relatedness, as well prevalence markers, consistent two...