A5061051950- Veterinary Oncology Research
- Clinical Laboratory Practices and Quality Control
- Hematological disorders and diagnostics
- Blood groups and transfusion
- Microbial infections and disease research
- Veterinary Equine Medical Research
- Platelet Disorders and Treatments
- Animal health and immunology
- Vector-borne infectious diseases
- Hemoglobinopathies and Related Disorders
- Infectious Diseases and Mycology
- Erythrocyte Function and Pathophysiology
- Bacterial Identification and Susceptibility Testing
- Veterinary Orthopedics and Neurology
- Digital Imaging for Blood Diseases
- Blood properties and coagulation
- Immune Response and Inflammation
- Hemodynamic Monitoring and Therapy
- Orthopedic Infections and Treatments
- Mycobacterium research and diagnosis
- Renal function and acid-base balance
- Vector-Borne Animal Diseases
- Veterinary Medicine and Surgery
- Glycosylation and Glycoproteins Research
- Inflammatory Biomarkers in Disease Prognosis
Swedish University of Agricultural Sciences
2013-2022
Uppsala University
2012-2013
Veterinary Medical Center
2013
University of Pennsylvania
2011
Michigan State University
1990-2001
University of Guelph
2000
St. Luke's Hospital
1999
Biopure (United States)
1999
Virginia–Maryland College of Veterinary Medicine
1988
Virginia Tech
1988
A study was carried out to test the accuracy and consistency of veterinary pathologists, not specialists in hematopathology, applying World Health Organization (WHO) system classification canine lymphomas. This represents an initiative ACVP Oncology Committee, has been endorsed by Small Animal Veterinary Association (WASVA). Tissue biopsies from cases lymphoma were received oncologists, a pathologists given only signalment on 300 cases. Twenty reviewed these with each required choose...
The Sysmex XT-2000iV is a laser-based, flow cytometric hematology system that has been introduced for use in large and referral veterinary laboratories.The purpose of this study was to validate the counting erythrocytes, reticulocytes, platelets, total leukocytes blood from ill dogs, cats, horses.Blood samples diseased animals (133 65 73 horses) were analyzed with CELL-DYN 3500. Manual reticulocyte counts done on an additional 98 canine 14 feline manual platelet 55 samples, compared...
Measurement of C-reactive protein (CRP) is used for diagnosing and monitoring systemic inflammatory disease in canine patients. An automated human immunoturbidimetric assay has been validated measuring CRP, but cross-reactivity with CRP unpredictable.The purpose the study was to validate a new canine-specific method (Gentian cCRP).Studies imprecision, accuracy, prozone effect, interference, limit quantification, stability under different storage conditions were performed. The compared...
Counting mitotic figures (MF) in hematoxylin and eosin–stained histologic sections is an integral part of the diagnostic pathologist’s tumor evaluation. The count (MC) used alone or as a grading scheme for assessment prognosis clinical decisions. Determining MCs subjective, somewhat laborious, has interobserver variation. Proposals standardizing this parameter veterinary field are limited to terminology (use term MC) area (MC counted measuring 2.37 mm 2 ). Digital imaging techniques now...
Standardization of tumor assessment lays the foundation for validation grading systems, permits reproducibility oncologic studies among investigators, and increases confidence in significance study results. Currently, there is minimal methodological standardization assessing tumors veterinary medicine, with few attempts to validate published protocols schemes. The current article address these shortcomings by providing standard guidelines parameters evaluating specific types. More detailed...
Background: The Sysmex XT‐2000iV is a laser‐based, flow cytometric hematology system that stains nucleic acids in leukocytes with fluorescent dye. A 4‐part differential obtained using side fluorescence light and laser scatter. Objective: purpose of this study was to validate the for determining leukocyte counts blood from ill dogs, cats, horses. Methods: Blood samples diseased animals (133 65 73 horses) were analyzed (Auto‐diff) CELL‐DYN 3500. Manual differentials by counting 100...
Most automated hematology analyzers cannot detect canine or feline basophils. However, many veterinary laboratories continue to report basophils as part of the 5-part differential leukocyte count for dogs and cats.The study objectives were evaluate performance Sysmex XT-2000iV, Advia 2120, CELL-DYN 3500 in detecting using blood from dogs, cats, rabbits with basophilia investigate concurrence other hematologic changes, sex, breed dogs.One more 3 was used analyze 11 samples prominent (≥ 5%)...
Many Cavalier King Charles Spaniel (CKCS) dogs are affected by an autosomal recessive dysplasia of platelets resulting in fewer but larger platelets. The IDEXX Vet Autoread (QBC) hematology analyzer directly measures the relative volume a blood sample (plateletcrit). We hypothesized that CKCS both with and without hereditary macrothrombocytosis would have normal plateletcrit QBC results better identify total circulating CKSC than methods enumerating platelet numbers.The major purpose this...
Background The neurotransmitter serotonin (5‐hydroxytryptamine, 5‐ HT ) has recently been suggested to play a role in the development of naturally acquired myxomatous mitral valve disease ( MMVD dogs. Aim To investigate association between serum concentration and severity dogs, assess potential associations concentrations dog characteristics, echocardiographic variables, heart rate, systolic blood pressure, presence macrothrombocytosis, plateletcrit. Animals A total 120 client‐owned Material...
Delayed analysis of blood samples may be caused by restricted access to laboratories. Artifactual changes occur in the measured analytes as a consequence delayed and complicate interpretation data.The purpose this study was characterize artifactual equine blood, due storage, using Advia 120 hematology analyzer.Samples from 5 horses were analyzed soon after collection again 24 48 hours storage at either 4 degrees C or ambient laboratory temperature ( approximately C).Delayed resulted...
Summary A breeding colony was established to investigate the inheritance of associated ocular and skeletal dysplasia in Labrador Retrievers; 124 pups were produced. These evaluated for presence lesions, including cataracts, vitreous strands, persistent hyaloid remnants, retinal folds, dysplasia, peripapillary hyperreflectivity, rhegmatogenous detachments, abnormality, which recognized by shorter than normal forelimbs an abnormal morphologic appearance radius ulna. Analysis distribution...
A severe regenerative anemia was detected in a 12-week-old mixed breed puppy Sweden. small protozoan parasite observed erythrocytes on blood smear. It initially suspected to be Babesia gibsoni based its size and because B. previously recorded Surprisingly, specific polymerase chain reaction analysis identified the as Theileria annae. T. annae is endemic Northwest Spain, very uncommonly reported elsewhere has never been Scandinavia. dogs used for dog fighting, it thought transmitted by bites....
In a dog with joint pain, it is important to determine whether has suppurative disease, characterized by exudation of neutrophils in the synovial fluid, or not, as this affects choice diagnostic tests and treatments. The aim study was evaluate measurement serum C-reactive protein (CRP) concentration could be used discriminate between dogs arthritis osteoarthritis (OA). Furthermore, concentrations fluid interleukin (IL) 6 were measured disease healthy dogs, correlated CRP concentrations.Dogs...
Background: For differential leukocyte counts, automated blood smear evaluation systems have been too slow or inaccurate to replace supplement the manual count. The CellaVision DM96Vision (DM96V), a new instrument, is an image analysis system that rapid and accurate enough be used for enumerating human leukocytes may useful of canine blood. Objectives: aims this study were evaluate performance DM96V in counting leukocytes, compare its with other methods, analyze interoperator variability....
Background Cytologic examination of smears prepared from ocular swabs conjunctiva cats with conjunctivitis permits identification the type inflammation and possibly specific microorganisms. Results studies diagnostic utility cytology for detection infectious causes feline have been inconsistent. Objectives The objectives this study were to describe cytologic findings in compare those results PCR analysis herpesvirus ( FHV ‐1), C hlamydophila felis ) , M ycoplasma ). Methods Conjunctival 88...
Abstract Background Neutrophil myeloperoxidase content is determined by the Advia 2120 hematology system staining characteristics. Changes in are shown location of neutrophils on peroxidase dot plots and as index ( MPXI ). Significant changes have been reported during severe inflammation horses, dogs, people but conclusions were inconsistent. Objectives Infusion endotoxin was used to initiate an inflammatory stimulus under controlled conditions over a longer time period than previous studies...
The mean platelet volume (MPV) was evaluated in 68 dogs with thrombocytopenia attributable to various causes. Platelet size high or low some dogs. most clinically useful observation that MPV (microthrombocytosis) a specific indicator of immune-mediated (IMT) these thrombocytopenic All but one case microthrombocytosis (MPV less than 5.4 fl) found IMT. Microthrombocytosis detected 17 31 IMT and appeared at the onset disease. Macrothrombocytosis greater 9.4 indicated active thrombopoiesis, not...
To determine the effect of platelet count on accurate assessment serum electrolyte concentrations, simultaneous counts and determinations were performed plasma from 40 dogs. Dogs grouped according to as follows: thrombocytopenic (less than 150,000/microliters), normal (150,000 600,000/microliters), or thrombocytotic (greater 600,000/microliters). Serum potassium concentration was significantly higher in dogs (mean difference, 0.63 +/- 0.17 mEq/l) with thrombocytosis 1.55 0.73 mEq/l). This...
An in-clinic assay for equine serum amyloid A (SAA) analysis, Equinostic EVA1, was evaluated use in a clinical setting. Stability of SAA samples determined. Intra- and inter- variation the method The (EVA1) results were compared to reference (Eiken LZ SAA) with 62 patient samples. For concentrations within range EVA1 (10-270 mg/L), differences between methods difference plot. Linearity under dilution two three pools stored at 4°C approximately 22°C day 0, 1, 2, 4, 7, 17 analysed two-way...
The performance of a digital Atago PAL-USG Cat refractometer (Atago) was compared with Schmidt and Haensch, Goldberg type (S+H).Specific gravity 47 canine feline urine samples determined both refractometers the results were Passing-Bablok Bland-Altman plots. In addition, specific dilutions 10% glucose, NaCl, 3% albumin solutions expected values.Both consistently reported 1.000 distilled water. correlation between based on plots excellent (r = .99), but, in plots, there significant,...