A5053177932- Gene expression and cancer classification
- Molecular Biology Techniques and Applications
- Chemical Synthesis and Analysis
- Click Chemistry and Applications
- Melanoma and MAPK Pathways
- Enzyme function and inhibition
- Cancer Genomics and Diagnostics
- Cytokine Signaling Pathways and Interactions
- Cancer Cells and Metastasis
- bioluminescence and chemiluminescence research
- Cutaneous Melanoma Detection and Management
- Genomics and Chromatin Dynamics
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- RNA Interference and Gene Delivery
- Pancreatic and Hepatic Oncology Research
- DNA Repair Mechanisms
- Advanced Computing and Algorithms
- Cancer-related Molecular Pathways
- Advanced Biosensing Techniques and Applications
- Ubiquitin and proteasome pathways
- Multiple Myeloma Research and Treatments
- RNA Research and Splicing
- Protein Degradation and Inhibitors
- HER2/EGFR in Cancer Research
Edward Via College of Osteopathic Medicine
2023-2024
Moffitt Cancer Center
2004-2017
University of South Florida
2003-2013
University of Florida
2010-2011
University of Arizona
2010-2011
Molecular Oncology (United States)
2003-2010
Florida College
2005
National Cancer Institute
1999-2000
National Institutes of Health
1997-2000
Purdue University West Lafayette
1990
The process of malignant transformation, progression and metastasis melanoma is poorly understood. Gene expression profiling human cancer has allowed for a unique insight into the genes that are involved in these processes. Thus, we have attempted to utilize this approach through analysis series primary, non-metastatic cutaneous tumors metastatic samples.We utilized gene microarray variety molecular techniques compare 40 (MM) samples, composed 22 bulky, macroscopic (replaced) lymph node...
Abstract A key step in bringing gene expression data into clinical practice is the conduct of large studies to confirm preliminary models. The performance such confirmatory and transition requires that microarray from different laboratories are comparable reproducible. We designed a study assess comparability four will larger profiling confirmation project lung adenocarcinomas. To test feasibility combining across laboratories, frozen tumor tissues, cell line pellets, purified RNA samples...
NF-κB is constitutively activated in many cancer types and a potential key mediator of tumor-associated inflammation, tumor growth, metastasis. We investigated the role cell activity T cell–mediated antitumor responses. In tumors rendered immunogenic by model antigen expression or following administration vaccines, we found that high leads to rejection and/or growth suppression mice. Using global RNA microarray, demonstrated enhanced several chemokines, including Ccl2, decreased CCL2 was...
Abstract Purpose: To develop targeted molecular imaging probes for the noninvasive detection of breast cancer lymph node metastasis. Experimental Design: Six cell surface or secreted markers were identified by expression profiling and from literature as being highly expressed in metastases. Two these carbonic anhydrase isozymes (CAIX and/or CAXII) validated protein immunohistochemistry patient tissue samples on a microarray containing 47 normal samples, 42 ductal carcinoma situ, 43 invasive...
// Allison S. Cohen 1 , Farah K. Khalil 2 Eric A. Welsh 3 Matthew B. Schabath 4 Steven Enkemann 5 Andrea Davis Jun-Min Zhou 6 David C. Boulware Jongphil Kim 7, 9 Haura 8 and L. Morse 1, 9, 10 Department of Cancer Imaging Metabolism, H. Lee Moffitt Center Research Institute, Tampa, FL, USA Anatomic Pathology, Biomedical Informatics Shared Resource, Epidemiology, Molecular Genomics Biostatistics 7 Biostatistics, Thoracic Oncology, Oncologic Sciences, College Medicine, University South Florida,...
Early detection of colorectal cancer (CRC) is crucial for effective treatment. Among CRC screening techniques, optical colonoscopy widely considered the gold standard. However, it a costly and invasive procedure with low rate compliance. Our long-term goal to develop molecular imaging agents non-invasive by imaging-based using CT, MRI or fluorescence. To achieve this, cell surface targets must be identified validated. Here, we report discovery cell-surface markers that distinguish from...
Recent studies suggest that growth inhibition by 1,25-dihydroxyvitamin D3 represents an innovative approach to ovarian cancer therapy. To understand the molecular mechanism of action, we profiled hormone-induced changes in transcriptome cells using microarray technology. More than 200 genes were identified be regulated D3. Reverse transcription-PCR analyses confirmed regulation a group apoptosis-related genes, including up-regulation decoy receptor inhibits tumor necrosis factor-related...
Pathologic axillary lymph node (ALN) status is an important prognostic factor for staging breast cancer. Currently, determined by histopathology following surgical excision of sentinel node(s), which invasive, time consuming, and costly procedure with potential morbidity to the patient. Here, we describe imaging platform noninvasive assessment ALN status, eliminating need examination patients rule out nodal involvement. A targeted probe (MamAb-680) was developed conjugation a...
Abstract Purpose: Adenocarcinoma arises in Barrett's esophagus by progression from metaplasia to cancer through grades of dysplasia. Our aim this exploratory study was characterize the broad changes gene expression that underlie histologic and assess potential for using these as a marker predictive malignant epithelium. Experimental Design: Microarray analysis used obtain individual profiles endoscopic biopsies nine esophageal adenocarcinomas epithelia which three cancers had arisen. Pooled...
BACKGROUND Gene expression profiling using gene-discovery, high-density microarray technologies is a powerful tool. One potential application the development of tumor classifiers that predict site origin. For this technology to be relevant, however, it must applicable biopsy samples, which most often are fine-needle aspiration (FNAB) samples. METHODS Surgically resected tumors were sampled by FNAB different gauge needles. A portion excised was also collected. RNA samples extracted standard...
Abstract The farnesyl transferase inhibitor R115777 has been found to have clinical activity in diverse hematopoietic tumors. Clinical efficacy, however, does not correlate with Ras mutation status or inhibition of transferase. To further elucidate the mechanisms by which induces apoptosis and investigate drug resistance, we identified characterized a R115777-resistant human myeloma cell line. 8226/R5 cells were be at least 50 times more resistant compared parent line 8226/S. K-Ras remained...
In the United States, lung cancer is leading cause of death and ranks second in number new cases annually among all types cancers. Better methods or tools for diagnosing treating this disease are needed to improve patient outcomes. The delta-opioid receptor (δOR) reported be overexpressed cancers not expressed normal lung. Thus, we decided develop a cancer-specific imaging agent targeting receptor. We have previously developed δOR-targeted fluorescent based on synthetic peptide antagonist...
Our study examines the effect of apoptosis on prothymosin alpha, an abundant, nuclear protein intimately involved with proliferation all mammalian cells. When HeLa cells were treated actinomycin D, etoposide, or staurosporine following synchronization hydroxyurea, they underwent based several specific criteria, including fragmentation DNA and activation caspases. Similarly NIH3T3 arrested displayed no indicators apoptosis. In HeLa, but not in cells, alpha levels declined precipitously a...
The melanocortin 1 receptor (MC1R) is overexpressed in most melanoma metastases, making it a promising target for imaging of melanomas. In this study, the expression MC1R large fraction patients with was confirmed using mRNA and tissue microarray. Here, we have characterized vivo tumor distribution pharmacokinetics (PK) uptake clearance specific peptidomimetic ligand conjugated to near-infrared fluorescent dye. We propose an interdisciplinary framework bridge different time space scales...
Prothymosin alpha is a small, unfolded, negatively charged, poorly antigenic mammalian protein with potent nuclear localization signal. Although it apparently essential for growth, its precise function unknown. We examined the location and behavior of bearing different epitope tags using in situ immunolocalization COS-1 NIH3T3 cells. Tagged prothymosin appeared to be punctate widely dispersed throughout nucleus, exception nucleolus. A tiny cytoplasmic component, which persisted presence...
ABSTRACT The hydrothermal vent gammaproteobacterium Thiomicrospira crunogena inhabits an unstable environment and must endure dramatic changes in habitat chemistry. This sulfur chemolithoautotroph responds to dissolved inorganic carbon (DIC) (DIC = CO 2 + HCO 3 − −2 ) availability with a carbon-concentrating mechanism (CCM) which whole-cell affinity for DIC, as well the intracellular DIC concentration, increases substantially under limitation. To determine whether this CCM is regulated at...
Human and monkey prothymosin α contain activated carbonyl groups on glutamic acid residues. Three lines of evidence indicate the existence unusual phosphates. 1) Prothymosin continued to be metabolically labeled with [32P]orthophosphoric despite a mutation at Ser1, sole site phosphate in purified bovine (Sburlati, A. R., De La Rosa, A., Batey, D. W., Kurys, G. L., Manrow, R. E., Pannell, L. K., Martin, B. M., Sheeley, Berger, S. (1993)Biochemistry 32, 4587–4596). 2) Immediately upon cell...