A5022149512- Drug Transport and Resistance Mechanisms
- Epilepsy research and treatment
- Pharmacological Effects and Toxicity Studies
- Malaria Research and Control
- Neuroscience and Neuropharmacology Research
- Mosquito-borne diseases and control
- Invertebrate Immune Response Mechanisms
- Insect symbiosis and bacterial influences
- Insect Resistance and Genetics
- Barrier Structure and Function Studies
- Pharmacogenetics and Drug Metabolism
- Insect Pest Control Strategies
- Neuroinflammation and Neurodegeneration Mechanisms
- Entomopathogenic Microorganisms in Pest Control
- S100 Proteins and Annexins
- Traumatic Brain Injury and Neurovascular Disturbances
- Nitric Oxide and Endothelin Effects
- Bacterial Genetics and Biotechnology
- Viral Infections and Vectors
- Zoonotic diseases and public health
- Drug-Induced Hepatotoxicity and Protection
- Nanoparticle-Based Drug Delivery
- Epigenetics and DNA Methylation
- Protein purification and stability
- Adrenal Hormones and Disorders
Cleveland Clinic Lerner College of Medicine
2016-2025
Case Western Reserve University
2018-2025
Cleveland Clinic
2009-2024
Tata Institute for Genetics and Society
2020-2024
University of Delhi
2003-2024
Institute for Stem Cell Biology and Regenerative Medicine
2020-2023
Vidyasagar University
2018-2023
Academy of Nutrition and Dietetics
2018
Neurological Surgery
2010-2013
Jawaharlal Nehru University
2011-2012
The acknowledgement of risks for traumatic brain injury in American football players has prompted studies sideline concussion diagnosis and testing neurological deficits. While concussions are recognized etiological factors a spectrum sequelae, the consequences sub-concussive events unclear. We tested hypothesis that blood-brain barrier disruption (BBBD) accompanying surge astrocytic protein S100B blood may cause an immune response associated with production auto-antibodies. also wished to...
It is widely accepted that climate change has a significant impact on the production of agricultural plants and insect pests associated with them. Some uncertainties about are related to small-scale climatic variability, including temperature variations, relative humidity, atmospheric CO2, precipitation patterns. Due their great diversity, serve as hosts, variations in global temperatures, different species expected react warming differently depending where they live. Numerous factors,...
P450 enzymes (CYPs) play a major role in hepatic drug metabolism. It is unclear whether these are functionally expressed by the diseased human blood-brain barrier (BBB) and involved local metabolism or response. We have evaluated cerebrovascular CYP expression function, hypothesizing possible implication drug-resistant epilepsy.
Purpose: Compelling evidence supports the presence of P450 enzymes (CYPs) in central nervous system (CNS). However, little information is available on localization and function CYPs drug-resistant epileptic brain. We have evaluated pattern expression specific enzyme CYP3A4 studied its co-localization with MDR1. also determined whether an association exists between cell survival. Methods: Brain specimens were obtained from eight patients undergoing resection to relieve seizures or remove a...
Increased blood-brain barrier (BBB) permeability is radiologically detectable in regions affected by drug-resistant epileptogenic lesions. Brain penetration of antiepileptic drugs (AEDs) may be BBB damage. We studied the effects damage on brain distribution hydrophilic [deoxy-glucose (DOG) and sucrose] lipophilic (phenytoin diazepam) molecules. tested hypothesis that drug differentially
Purpose: A link between seizure susceptibility, blood–brain barrier (BBB) failure, and the activation of peripheral white blood cells has been recently proposed. However, molecular players involved in this cascade events are unknown. We tested hypothesis that immunosupression by splenectomy or lack perforin, a downstream factor natural killer (NK) cytotoxic T cells, could reduce onset. Methods: Pilocarpine was used to induce seizures adult rats wild-type perforin-deficient mice. Splenectomy...
Summary Objective Recent evidence suggests a metabolic contribution of cytochrome P450 enzymes ( CYP s) to the drug‐resistant phenotype in human epilepsy. However, upstream molecular regulators epileptic brain remain understudied. We therefore investigated expression and function pregnane xenobiotic PXR ) glucocorticoid GR nuclear receptors endothelial cells established from post‐epilepsy surgery samples. Methods / localization was evaluated by immunohistochemistry specimens subjects who...
S100B is a reporter of blood-brain barrier (BBB) integrity which appears in blood when the BBB breached. Circulating derives from either extracranial sources or release into circulation by normal fluctuations pathologic disruption (BBBD). Elevated matches clinical presence indices BBBD (gadolinium enhancement albumin coefficient). After repeated sub-concussive episodes, serum triggers an antigen-driven production anti-S100B autoantibodies. We tested hypothesis that tissue due to peripheral...
Summary Purpose Brain drug bioavailability is regulated by the blood–brain barrier ( BBB ). It was recently suggested that cytochrome P 450 CYP ) enzymes could act in concert with multidrug transporter proteins to regulate penetration and distribution into diseased brain. The possibility p hase II metabolic be expressed epileptic brain has been not evaluated. Phase are involved metabolism of common antiepileptic drugs AED s). Methods enzyme UGT 1 A 4 expression evaluated temporal lobe...
Anopheles stephensi is the most menacing malaria vector to watch for in newly urbanising parts of world. Its fitness reported be a direct consequence adapting laying eggs over-head water tanks with street-side puddles polluted by oil and sewage. Large frequent inversions genome vectors are implicated adaptation. We report assembly strain An. type-form, collected from construction site Chennai (IndCh) 2016. The here L50 4, completes trilogy high-resolution genomes strains respect 16.5 Mbp 2Rb...
Summary Objective Available evidence points to a role of cytochrome P450 (Cyp) drug biotransformation enzymes in central nervous system diseases, including epilepsy. Deviations pharmacokinetic profiles may impact therapeutic outcomes. Here, we ask whether spontaneous recurrent seizure ( SRS ) activity is sufficient modulate the expression major Cyp liver and brain. Methods Unilateral intrahippocampal (IH) kainic acid KA injections were used elicit nonconvulsive status epilepticus SE ),...