A5065687542- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Advanced Biosensing Techniques and Applications
- Microfluidic and Capillary Electrophoresis Applications
- RNA Interference and Gene Delivery
- Microfluidic and Bio-sensing Technologies
- Asymmetric Synthesis and Catalysis
- Chemical Synthesis and Reactions
- Chemical Synthesis and Analysis
- Innovative Microfluidic and Catalytic Techniques Innovation
- Synthesis and bioactivity of alkaloids
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Synthesis and biological activity
- Synthesis and Biological Evaluation
- Analytical Chemistry and Sensors
- Fluorine in Organic Chemistry
- bioluminescence and chemiluminescence research
- Analytical Chemistry and Chromatography
- Nanopore and Nanochannel Transport Studies
- Synthesis and Reactivity of Sulfur-Containing Compounds
- Cardiac electrophysiology and arrhythmias
- Electrochemical Analysis and Applications
- Synthetic Organic Chemistry Methods
- Synthesis and Characterization of Heterocyclic Compounds
Hokkaido University
2016-2025
Faculty (United Kingdom)
2015-2021
Engineering (Italy)
2015-2021
Sapporo Science Center
2015-2021
Tokyo University of Science
2012-2013
Shimizu (Japan)
2011
Sendai City Hospital
1996-2011
Japan Science and Technology Agency
2010
Gene Therapy Laboratory
2009
Anges (Japan)
2009
The precise size control of the lipid nanoparticle (LNP)-based nanodrug delivery system (DDS) carriers, such as 10 nm tuning LNPs, is one major challenge for development next-generation nanomedicines. Size-controlled LNPs would realize size-selective tumor targeting and deliver DNA RNA to target tissues effectively by passing through stromal cells. Herein, we developed a baffle mixer device named invasive production device, or iLiNP short, which has simple two-dimensional microchannel...
Lipid nanoparticles (LNPs) or liposomes are the most widely used drug carriers for nanomedicines. The size of LNPs is one essential factors affecting delivery efficiency and therapeutic efficiency. Here, we demonstrated effect lipid concentration mixing performance on LNP using microfluidic devices with aim understanding formation mechanism controlling precisely. We fabricated different depths, 11 μm 31 μm, their chaotic micromixer structures. According to behavior results, by a low solution...
Formation behavior was discussed for lipid nanoparticles (LNPs) in the microfluidic devices equipped with different cycle numbers of micromixers.
Microfluidic methodologies for preparation of lipid nanoparticles (LNPs) based on an organic solvent injection method enable precise size control the LNPs. After LNPs, needs some post-treatments, such as overnight dialysis or direct dilution with a buffer solution. LNP production using microfluidic-based is dominated by kinetics rather than thermodynamics. Kinetics ethanol removal from inner and outer membranes LNPs could induce structural change in membrane that lead to fusion However,...
Microfluidic devices are widely used in lipid nanoparticle (LNP)-based vaccines and nanomedicine research. These should be stiff enough to withstand the high flow rate for mass production of LNPs, malleable use when fabricating complicated microchannel or micromixer structures, such as staggering herringbone micromixers. Due limitations available fabrication methods, optimal microfluidic have not yet been developed. In this study, we report development a glass-based device based on invasive...
Lipid nanoparticles (LNPs) are essential carrier particles in drug delivery systems, particularly ribonucleic acid delivery. In preparing lipid-based nanoparticles, microfluidic-based ethanol injection may produce precisely size-controlled nanoparticles. Ethanol is critical LNP formation and post-treatment processes affects liposome size, structure, lamellarity, drug-loading efficiency. However, the effects of time-dependent changes concentration on structural dynamics liposomes not clearly...
Abstract ZIt was found that, in the presence of TiCl4,1-trimethylsiloxy-1,3-butadiene (II) reacts with acetals(I) to give δ-alkoxy-δ,β-unsaturated aldehydes good yields. This reaction provides a novel method for introduction of–CH2–CH=CH-CHO unit an electrophile as acetal activated by TiCl4.
This paper describes a simple and instrument-free screen-printing method to fabricate hydrophilic channels by patterning polydimethylsiloxane (PDMS) onto chromatography paper. Clearly recognizable border lines were formed between hydrophobic areas. The minimum width of the printed channel deliver an aqueous sample was 600 μm, as obtained this method. Fabricated microfluidic paper-based analytical devices (μPADs) tested for several colorimetric assays pH, glucose, protein in both buffer...
The combination of a microfluidic paper-based analytical device (μPAD) and digital image analysis is widely used for quantitative with μPADs because its easy simple operation. Herein, we have demonstrated based on multiple color changes μPAD. CIE L*a*b* system was employed to analyse the images obtained We made pH measurements using universal pH-indicator showing various values aqueous test solutions. detectable range this method wider than typical grayscale-based analysis, succeeded in wide 2-9.
Lithium carbonate is an effective medicine for the treatment of bipolar disorder, but concentration lithium in patient's blood must be frequently monitored because its toxicity. To date, no colorimetric methods ion detection whole without pretreatment have been reported. Here, we report a paper-based device that allows point-of-care testing one step. This composed two elements linked to each other: cell separation unit and unit. After portion has placed on end unit, plasma sample...
Connexin43 Expression in DCM. Introduction: Gap junction alterations recently have been implicated chronic heart failure, but direct evidence between gap manifestation nonischemic dilated cardiomyopathy (DCM) is lacking. The current study examines whether qualitative changes or altered distribution of junctional connexin43 (Cx43) are related to global ventricular function and arrhythmia Methods Results: We investigated 31 DCM patients ( 52 ± 15 years ) 5 control subjects 55 10 ). Cx43...
To clarify the influence of hypertension and blood pressure (BP) control on thromboembolism major hemorrhage in patients with nonvalvular atrial fibrillation, a post hoc analysis J-RHYTHM Registry was performed.A consecutive series outpatients fibrillation enrolled from 158 institutions. Of 7937 patients, 7406 (70.8% men, 69.8±10.0 years) were followed for 2 years or until an event occurred. Hypertension defined as systolic BP ≥140 mm Hg, diastolic ≥90 history hypertension, and/or...
Abstract A new synthesis of vitamin was successfully accomplished by reducing retinal, prepared the reaction 3-methyl-1-trimethylsiloxy-1,3-butadiene (III) with β-ionylideneacetaldehyde dimethyl acetal (VII) followed elimination methanol tertiary amine such as 1,5-diazabicyclo[5.4.0]undecene-5. The intermediate, (V), also according to same procedure starting from β-cyclocitral (II).
A compact and lightweight liquid chromatography system is presented with overall dimensions of 26 cm width × 18 length 21 height weight 2 kg. This comprises a battery-operated electroosmotic pump, manual injector, microfluidic chip device containing packed column an electrochemical detector, USB bus-powered potentiostat. The pumping was designed for microfluidic-based reversed-phase in which electroosmotically generated water stream pushes the mobile phase via diaphragm output. flow rate...
RNA and DNA delivery technologies using lipid nanoparticles (LNPs) have advanced significantly, as demonstrated by their successful application in mRNA vaccines. To date, commercially available therapeutics include Onpattro, a 21 bp siRNA, vaccines comprising 4300 nucleotides for COVID-19. However, significant challenge remains achieving efficient transfection, the size of delivered increases. In contrast to plasmid (pDNA) transfection requires multiple steps, including cellular uptake,...
Abstract Dienoxysilanes (2) react with acetals in the presence of TiCl4 and also coexistence Ti(OiPr)4 to give δ-alkoxy-α,β-unsaturated aldehydes (3–5) good yields. In 1,8-diazabicyclo[5.4.0] undec-7-ene or 1,5-diazabicyclo[4.3.0]non-5-ene molecular sieves 3A 4A, elimination reaction δ-alkoxyl group α,β-unsaturated proceeds smoothly afford corresponding polyenals
Ochratoxin A (OTA) is one of the most abundant food-contaminating mycotoxins that also a potential carcinogen and responsible for many diseases affecting humans. Consequently, sensitive, portable device detection OTA highly desirable. In this study, miniaturized electrochemical aptamer-based sensor was developed label-free, sensitive OTA. For construction sensor, gold thin-film three-electrode system fabricated using standard microfabrication techniques on polystyrene substrate (25 mm × 25...
Sub 100 nm-sized lipid nanoparticles (LNPs) have been widely used in drug delivery systems (DDSs). The size of the LNPs is an important parameter for DDS performance, such as biodistribution and gene silencing using siRNAs. However, prepared by conventional preparation method show a wide distribution. To improve LNP distribution, we developed microfluidic device, named iLiNP™ previous study. This device could produce range 20 to 150 nm, but distribution large-sized needs be further improved....