A5110746698- Histone Deacetylase Inhibitors Research
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Mitochondrial Function and Pathology
- Circular RNAs in diseases
- Cancer-related gene regulation
- Autophagy in Disease and Therapy
- Cancer, Lipids, and Metabolism
- Telomeres, Telomerase, and Senescence
- Ubiquitin and proteasome pathways
- Gene expression and cancer classification
- Sirtuins and Resveratrol in Medicine
- Cancer, Hypoxia, and Metabolism
- RNA regulation and disease
- Carcinogens and Genotoxicity Assessment
- Protein Degradation and Inhibitors
- Extracellular vesicles in disease
- RNA and protein synthesis mechanisms
- Molecular Biology Techniques and Applications
- Lipoproteins and Cardiovascular Health
- PI3K/AKT/mTOR signaling in cancer
- Forensic Toxicology and Drug Analysis
Chungnam National University
2020-2025
Chonnam National University
2019-2024
Chonnam National University Hospital
2024
CHA University
2024
Soonchunhyang University
2024
Konkuk University Medical Center
2024
Catholic University of Korea
2006-2023
National Institute on Aging
2014-2022
National Institutes of Health
2014-2022
Institute on Aging
2015-2022
HuR influences gene expression programs and hence cellular phenotypes by binding to hundreds of coding noncoding linear RNAs. However, whether binds circular RNAs (circRNAs) impacts on their function is unknown. Here, we have identified en masse circRNAs in human cervical carcinoma HeLa cells. One the most prominent target was hsa_circ_0031288, renamed CircPABPN1 as it arises from PABPN1 pre-mRNA. Further analysis revealed that did not influence abundance; interestingly, however, high levels...
Accumulated evidence has established that aberrant regulation of histone deacetylases (HDACs) is one the major causes development human malignancies. Among different iso‐enzymes HDAC and sirtuins grouped as super family, little known to how deacetylase 2 (HDAC2) carcinogenesis in solid tumors. Here, order investigate possible role HDAC2 gastric carcinogenesis, we analyzed expression 71 adenocarcinomas by immunohistochemistry. Moderate strong was found 44 (62%) out a total The majority...
Sirtuins are nicotinamide adenine dinucleotide oxidized form (NAD+)-dependent deacetylases and function in cellular metabolism, stress resistance, aging. For sirtuin7 (SIRT7), a role ribosomal gene transcription is proposed, but its cancer has been unclear. In this study we show that SIRT7 expression was up-regulated large cohort of human hepatocellular carcinoma (HCC) patients. knockdown influenced the cell cycle caused significant increase liver cells to remain G1/S phase suppress growth....
Senescent cell accumulation in aging tissues is linked to age-associated diseases and declining function, prompting efforts eliminate them. Mass spectrometry analysis revealed that DPP4 (dipeptidyl peptidase 4) was selectively expressed on the surface of senescent, but not proliferating, human diploid fibroblasts. Importantly, differential presence allowed flow cytometry-mediated isolation senescent cells using anti-DPP4 antibodies. Moreover, antibody-dependent cell-mediated cytotoxicity...
Some mitochondrial long noncoding RNAs (lncRNAs) are encoded by nuclear DNA, but the mechanisms that mediate their transport to mitochondria poorly characterized. Using affinity RNA pull-down followed mass spectrometry analysis, we found two RNA-binding proteins (RBPs), HuR (human antigen R) and GRSF1 (G-rich sequence-binding factor 1), associated with DNA-encoded lncRNA RMRP mobilized it mitochondria. In cultured human cells, bound in nucleus mediated its CRM1 (chromosome region maintenance...
The function of the vast majority mammalian long noncoding (lnc) RNAs remains unknown. Here, analysis a highly abundant lncRNA, OIP5-AS1, known as cyrano in zebrafish, revealed that OIP5-AS1 reduces cell proliferation. In human cervical carcinoma HeLa cells, RNA-binding protein HuR, which enhances proliferation, associated with and stabilized it. Tagging MS2 hairpins to identify microRNAs miR-424 interacted competed HuR for binding OIP5-AS1. We further identified 'sponge' high levels...
Abstract Noncoding RNAs (ncRNAs) and RNA-binding proteins are potent post-transcriptional regulators of gene expression. The ncRNA 7SL is upregulated in cancer cells, but its impact upon the phenotype cells unknown. Here, we present evidence that forms a partial hybrid with 3′-untranslated region (UTR) TP53 mRNA, which encodes tumor suppressor p53. interaction mRNA reduced p53 translation, as determined by analyzing expression levels, nascent translation association polysomes. Silencing led...
Abstract Histone deacetylase 2 (HDAC2) is crucial for embryonic development, affects cytokine signaling relevant immune responses, and often significantly overexpressed in solid tumors, but little known of its role human lung cancer. In this study, we demonstrated the aberrant expression HDAC2 cancer tissues investigated oncogenic properties cell lines. inactivation resulted regression tumor growth activation cellular apoptosis via p53 Bax Bcl2 suppression. cycle regulation, caused induction...
Aging | doi:10.18632/aging.100834. Kotb Abdelmohsen, Amaresh C. Panda, Supriyo De, Ioannis Grammatikakis, Jiyoung Kim, Jun Ding, Ji Heon Noh, Kyoung Mi Julie A. Mattison, Rafael de Cabo, Myriam Gorospe
The primarily neuronal RNA-binding protein HuD is implicated in learning and memory. Here, we report the identification of several target transcripts linked to Alzheimer's disease (AD) pathogenesis. interacted with 3′ UTRs APP mRNA (encoding amyloid precursor protein) BACE1 β-site APP-cleaving enzyme 1) increased half-lives these mRNAs. also associated stabilized long noncoding (lnc)RNA BACE1AS, which partly complements enhances expression. Consistent promoting production enzyme, levels APP,...
Background: Neuronal death is a major hallmark of Alzheimer's disease (AD). Necroptosis, as programmed necrotic process, activated in AD. However, what signals and factors initiate necroptosis AD largely unknown. Methods: We examined the expression levels critical molecules necroptotic signaling pathway by immunohistochemistry (IHC) staining immunoblotting using brain tissues from patients mouse models APP/PS1 5×FAD. performed stereotaxic injection with recombinant TNF-α, anti-TNFR1...
Abstract By interacting with proteins and nucleic acids, the vast family of mammalian circRNAs is proposed to influence many biological processes. Here, RNA sequencing analysis differentially expressed during myogenesis revealed that circSamd4 expression increased robustly in mouse C2C12 myoblasts differentiating into myotubes. Moreover, silencing circSamd4, which conserved between human mouse, delayed lowered myogenic markers cultured from both species. Affinity pulldown followed by mass...
Ubiquitin-binding histone deacetylase 6 (HDAC6) is uniquely endowed with tubulin activity and plays an important role in the clearance of misfolded protein by autophagy. In cancer, HDAC6 has become a target for drug development due to its major contribution oncogenic cell transformation. present study we show that expression was down-regulated large cohort human hepatocellular carcinoma (HCC) patients, low significantly associated poor prognosis HCC patients 5-year overall, disease-free,...
Histone deacetylases (HDACs) are known to play a central role in the regulation of several cellular properties interlinked with development and progression cancer. Recently, HDAC1 has been reported be overexpressed hepatocellular carcinoma (HCC), but its biological roles hepatocarcinogenesis remain elucidated. In this study, we demonstrated overexpression subset human HCCs liver cancer cell lines. inactivation resulted regression tumor growth activation caspase-independent autophagic death,...
Histone deacetylase 2 (HDAC2) is crucial for embryonic development, affects cytokine signaling relevant immune responses and often significantly overexpressed in solid tumors; but little known about its role human hepatocellular carcinoma (HCC). In this study, we showed that targeted-disruption of HDAC2 resulted reduction both tumor cell growth de novo DNA synthesis Hep3B cells. We then demonstrated regulated cycle disruption caused G1/S arrest cycle. transition, selectively induced the...
// Hun-Jun Park 1, * , Ji Heon Noh 2, 3, Jung Woo Eun Yoon-Seok Koh 1 Suk Min Seo Won Sang 2 Young Lee Kiyuk Chang Ki Bae Seung Pum-Joon Kim Nam 4 Department of Cardiology, College Medicine, The Catholic University Korea, Seoul, Republic Korea Pathology, 3 Functional RNomics Research Center, Cancer Evolution These authors have contributed equally to this work Correspondence to: Nam, e-mail: swnam@catholic.ac.kr Kim, bjheart@catholic.ac.kr Keywords: ST-segment-elevation myocardial infarction,...
Skeletal muscle contains long multinucleated and contractile structures known as fibers, which arise from the fusion of myoblasts into myotubes during myogenesis. The myogenic regulatory factor (MRF) MYF5 is earliest to be expressed myogenesis functions a transcription in progenitor cells (satellite cells) myocytes. In mouse C2C12 myocytes, implicated initial steps myoblast differentiation myotubes. Here, using ribonucleoprotein immunoprecipitation (RIP) analysis, we discovered novel...
Alzheimer's disease (AD) is the most common type of dementia. Amyloid β (Aβ) plaques, tau-containing neurofibrillary tangles, and neuronal loss leading to brain atrophy are pathologic hallmarks AD. Given importance early diagnosis, extensive efforts have been undertaken identify diagnostic prognostic biomarkers for Circulating extracellular vesicles (EVs) provide a platform "liquid biopsy" Here, we characterized RNA contents plasma EVs age-matched individuals who were cognitively normal...
Neurite dystrophy is a pathologic hallmark of Alzheimer's disease (AD). However, drug discovery targeting neurite protection in AD remains largely unexplored.Aβ-induced and mitochondrial damage assays were used to evaluate Aβ toxicity the neuroprotective efficacy natural compound salidroside (SAL). The 5×FAD transgenic mouse model was study function SAL. To verify direct target SAL, we surface plasmon resonance cellular thermal shift analyze drug-protein interaction.SAL ameliorates...
Aberrant regulation of histone deacetylase 2 (HDAC2) was reported for gastric cancers. However, responsive cancer genes in disease onset and progression are less understood. HDAC2 expression studied by quantitative RT-PCR Western blotting. The functional consequences knockdown on cell-cycle regulation, programmed cell death, gene target identification investigated flow cytometry, blotting, electron microscopy, anchorage-independent colony formation, migration assay whole-genome microarray....