A5009397498- Adipokines, Inflammation, and Metabolic Diseases
- Adipose Tissue and Metabolism
- Bone health and osteoporosis research
- Bone and Dental Protein Studies
- Muscle Physiology and Disorders
- Bone health and treatments
- Bone Metabolism and Diseases
- Cancer-related molecular mechanisms research
- Metabolism, Diabetes, and Cancer
- Circular RNAs in diseases
- Nutrition and Health in Aging
- PI3K/AKT/mTOR signaling in cancer
- Regulation of Appetite and Obesity
- Osteoarthritis Treatment and Mechanisms
- Bone Tissue Engineering Materials
- Neurogenetic and Muscular Disorders Research
- RNA Interference and Gene Delivery
- Traumatic Brain Injury Research
- Orthopaedic implants and arthroplasty
- Bone and Joint Diseases
- Respiratory Support and Mechanisms
- Protein Kinase Regulation and GTPase Signaling
- TGF-β signaling in diseases
- Chronic Kidney Disease and Diabetes
- Cancer Risks and Factors
University of Birmingham
2017-2025
Versus Arthritis
2020-2024
NIHR Birmingham Biomedical Research Centre
2022-2024
University Hospitals Birmingham NHS Foundation Trust
2022
Queen Elizabeth Hospital Birmingham
2018-2021
University of Exeter
2019
The Ohio State University Wexner Medical Center
2019
University College London
2019
Campbellsville University
2019
Oxford Health NHS Foundation Trust
2018
Increasing evidence suggests that inflammation plays a central role in driving joint pathology certain patients with osteoarthritis (OA). Since many OA are obese and increased adiposity is associated chronic inflammation, we investigated whether hip exhibited differential pro-inflammatory cytokine signalling peripheral local lymphocyte populations, compared to normal weight patients. No differences either blood or populations were found between normal-weight However, synovial fibroblasts...
Abstract By interacting with proteins and nucleic acids, the vast family of mammalian circRNAs is proposed to influence many biological processes. Here, RNA sequencing analysis differentially expressed during myogenesis revealed that circSamd4 expression increased robustly in mouse C2C12 myoblasts differentiating into myotubes. Moreover, silencing circSamd4, which conserved between human mouse, delayed lowered myogenic markers cultured from both species. Affinity pulldown followed by mass...
Abstract Adiposity and adipokines are implicated in the loss of skeletal muscle mass with age several chronic disease states. The aim this study was to determine effects human obese lean subcutaneous adipose tissue secretome on myogenesis metabolism cells derived from both young (18–30 yr) elderly (>65 individuals. Obese impaired old myoblasts but not myoblasts. Resistin prolifically secreted by myotube thickness nuclear fusion activation classical NFκB pathway. Depletion resistin...
Changes in cellular metabolism have been implicated mediating the activated fibroblast phenotype a number of chronic inflammatory disorders, including pulmonary fibrosis, renal disease and rheumatoid arthritis. The aim this study was therefore to characterise metabolic profile synovial joint fluid fibroblasts under both basal conditions cohort obese normal-weight hip OA patients. Furthermore, we sought ascertain whether modulation pathway could alter their activity. Synovium obtained from...
Adipokines have emerged as central mediators of insulin sensitivity and metabolism, in part due to the known association obesity with metabolic syndrome disorders such type 2 diabetes. Recent studies rodents identified novel adipokine vaspin playing a protective role inflammatory diseases by functioning promoter during stress. However, at present skeletal muscle adipose tissue expression humans is poorly characterised. Furthermore, functional has not been studied. Since major for...
Traumatic injury leads to an extension of the half-life circulating neutrophils. However, how quickly neutrophil apoptosis is delayed post-injury currently unknown, as are underlying mechanisms and factors that promote this lifespan. During ultra-early (≤1 h) acute (4–12 48–72 phases, we collected blood samples from 73 adult trauma patients. Following ex vivo culture, was measured, alongside caspase-3 activation expression anti-apoptotic protein Mcl-1. To identify may survival post-trauma,...
It is often desirable to characterise the morphology of myogenic cultures. To achieve this, surface area myotubes quantified, along with nuclear fusion index (NFI). Existing methods such quantification are time-consuming and subject error-prone human input. We have developed MyoCount, an open-source program that runs via freely available MATLAB Runtime quantifies myotube NFI. MyoCount allows user adjust its parameters account for differences in image quality, magnification colour channels...
Cigarette consumption negatively impacts bone quality and is a risk-factor for the development of multiple associated disorders, due to highly vascularised structure being exposed systemic factors. However, impact on electronic cigarette (e-cigarette) use, which contains high doses nicotine other compounds including flavouring chemicals, metal particulates carbonyls, poorly understood. Here, we present first evidence demonstrating e-cigarette vapour condensate (replicating changes in liquid...
<ns7:p>Background There are insufficient <ns7:italic>in vitro</ns7:italic> bone models that accommodate long-term culture of osteoblasts and support their differentiation to osteocytes. The increased demand for effective therapies diseases, the ethical requirement replace animals in research, warrants development such models. Here we present an in-depth protocol prepare, create maintain three-dimensional, vitro</ns7:italic>, self-structuring osteocytogenesis osteoblast survival (>1 year)....
Abstract Patients with chronic liver disease (CLD) often present significant frailty, sarcopenia, and impaired immune function. However, the mechanisms driving development of these age-related phenotypes are not fully understood. To determine whether accelerated biological aging may play a role in CLD, epigenetic, transcriptomic, phenotypic assessments were performed on skeletal muscle tissue cells CLD patients age-matched healthy controls. Accelerated was detected, as evidenced by an...
What is the central question of study? Is Vps34 a nutrient-sensitive activator mTORC1 in human skeletal muscle? main finding and its importance? We show that altering nutrient availability, via protein-carbohydrate feeding, does not increase kinase activity muscle. Instead, feeding increased Vps34-mTORC1 co-localization parallel to activity. These findings may have important implications understanding nutrient-induced activation muscle interaction with Vps34.The Class III PI3Kinase, Vps34,...
No AccessJournal of Urology1 Apr 1957Comparative Study Five Urographic Contrast Media Merall Roth, and Thomas A. Nicholson RothMerall Roth More articles by this author , NicholsonThomas View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)66617-5AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail © 1957 The American Urological Association Education Research, Inc.FiguresReferencesRelatedDetailsCited ByWoodruff M...
<ns3:p><ns3:bold>Background: </ns3:bold>There are insufficient <ns3:italic>in vitro</ns3:italic> bone models that accommodate long-term culture of osteoblasts and support their differentiation to osteocytes. The increased demand for effective therapies diseases, the ethical requirement replace animals in research, warrants development such models.</ns3:p><ns3:p> Here we present an in-depth protocol prepare, create maintain three-dimensional, vitro</ns3:italic>, self-structuring...
<ns3:p>Background There are insufficient <ns3:italic>in vitro</ns3:italic> bone models that accommodate long-term culture of osteoblasts and support their differentiation to osteocytes. The increased demand for effective therapies diseases, the ethical requirement replace animals in research, warrants development such models. Here we present an in-depth protocol prepare, create maintain three-dimensional, vitro</ns3:italic>, self-structuring osteocytogenesis osteoblast survival (>1 year)....
Background The mechanisms underlying the state of systemic immune suppression that develops following major trauma are poorly understood. A post-injury increase in circulating levels prostaglandin E 2 (PGE ) has been proposed as a contributory factor, yet few studies have addressed how influences PGE biology. Methods Blood samples from 95 traumatically-injured patients (injury severity score ≥8) were collected across pre-hospital (≤2 hours), acute (4-12 hours) and subacute (48-72 settings....
<b>Background:</b> The use of humidified oxygen via high flow nasal cannula (HFNC) to treat acute hypoxic respiratory failure (AHRF) is increasing and incorporated into 2017 British Thoracic Society guidelines. majority the evidence for its concerns high-level care patients. As expands ward environments a different demographic will be treated. benefits in this context are not established. <b>Aim:</b> To identify patients treated with HFNC AHRF on ward, stratify groups based demographics...
Age-related disorders of the musculoskeletal system including sarcopenia, osteoporosis and arthritis represent some most common chronic conditions worldwide, for which there remains a great clinical need to develop safer more efficacious pharmacological treatments. Collectively, these involve multiple tissues, skeletal muscle, bone, articular cartilage synovium within joint lining. In this review, we discuss potential oligonucleotide therapies combat unmet in by evaluating successes...