A5060633192- Neuroblastoma Research and Treatments
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Hippo pathway signaling and YAP/TAZ
- Muscle Physiology and Disorders
- Bone health and treatments
- Sirtuins and Resveratrol in Medicine
- Cancer, Hypoxia, and Metabolism
- Genetics and Neurodevelopmental Disorders
- Bone Tumor Diagnosis and Treatments
- Nanopore and Nanochannel Transport Studies
- Cancer therapeutics and mechanisms
- Erythrocyte Function and Pathophysiology
- Ion channel regulation and function
- Nanoplatforms for cancer theranostics
- Medical Imaging and Pathology Studies
- Bone and Joint Diseases
- Adipose Tissue and Metabolism
- Cancer Cells and Metastasis
- Neonatal Respiratory Health Research
- Zebrafish Biomedical Research Applications
- Cellular Mechanics and Interactions
- Microtubule and mitosis dynamics
- 3D Printing in Biomedical Research
- Oral and Maxillofacial Pathology
Città della Speranza Foundation
2018-2025
University of Padua
2023-2025
University of Siena
2014-2018
Institute of Genetics and Biophysics
2010
National Academies of Sciences, Engineering, and Medicine
2008
Even though SQSTM1 gene mutations have been identified in a consistent number of patients, the etiology Paget's disease bone (PDB) remains part unknown. In this study we analyzed 533 608 consecutive PDB patients from several regions, including high-prevalence area Campania (also characterized by increased severity PDB, higher familial cases, and peculiar phenotypic characteristics as giant cell tumor). Eleven different (Y383X, P387L, P392L, E396X, M401V, M404V, G411S, D423X, G425E, G425R,...
Neuroblastoma (NB) is a paediatric tumour of the sympathetic nervous system. Half all cases are defined high-risk with an overall survival less than 40% at 5 years from diagnosis. The lack in vitro models able to recapitulate intrinsic heterogeneity primary NB tumours has hindered progress understanding disease pathogenesis and therapy response.Here we describe establishment 6 patient-derived organoids (PDOs) cells biopsies capable self-organising structure resembling tissue origin.PDOs...
Abstract Background Tumor hypoxia stimulates release of extracellular vesicles (EVs) that facilitate short- and long-range intercellular communication metastatization. Albeit EVs are known features Neuroblastoma (NB), a metastasis-prone childhood malignancy the sympathetic nervous system, whether hypoxic can NB dissemination is unclear. Methods Here we isolated characterized from normoxic cell culture supernatants performed microRNA (miRNA) cargo analysis to identify key mediators biological...
The ever-growing need for improved in vitro models of human tissues to study both healthy and diseased states is advancing the use techniques such as 3D Bioprinting. We here present our results on development a vascularized perfusable tumor mimic studies early steps Neuroblastoma metastatic spread. used multi-material sacrificial bioprinting approach fabricate cell-laden structures developed customized perfusion system enabling maintenance growth viability constructs up 3 weeks. Cell...
Muscle-specific ankyrins 1 (sAnk1) are a group of small ankyrin isoforms, which sAnk1.5 is the most abundant. sAnk1 localized in sarcoplasmic reticulum (SR) membrane from where they interact with obscurin, myofibrillar protein. This interaction appears to contribute stabilize SR close myofibrils. Here we report structural and functional characterization skeletal muscles knockout mice (KO). Deletion did not change expression localization proteins 4- 6-mo-old KO mice. Structurally, main...
Extracellular vesicles (EVs) could serve as drug delivery systems in cancer treatment. We here develop a microfluidic device to efficiently load EVs with verteporfin, for neuroblastoma treatment enhance its therapeutic effectiveness.
The zebrafish ( Danio rerio ) is a valuable model organism for studying human biology due to its easy genetic manipulation and small size. It optically transparent shares similarities with humans, making it ideal developmental processes, diseases, drug screening via imaging-based approaches. Solid malignant tumors often contain hypoxic areas that stimulate the release of extracellular vesicles (EVs), lipid-bound structures released by cells into space, facilitate short- long-range...
Abstract Neuroblastoma (NB) is a pediatric tumor of neural crest origin accounting for 7% cancers and 15% cancer deaths. Solid malignant tumors commonly contain hypoxic areas that stimulate release extracellular vesicles (EVs), lipid bound secreted by cells into the space. In our study we demonstrated EVs from NB promote pro-metastatic features in vitro an vivo zebrafish model. We characterized microRNA cargo compared their expression hypoxia vs. normoxia, finding miR-210-3p was most...