A5023091172- Immune cells in cancer
- Advanced Fluorescence Microscopy Techniques
- Tissue Engineering and Regenerative Medicine
- Integrated Circuits and Semiconductor Failure Analysis
- Electrospun Nanofibers in Biomedical Applications
- Histone Deacetylase Inhibitors Research
- MicroRNA in disease regulation
- Ferroptosis and cancer prognosis
- Extracellular vesicles in disease
- Plasmonic and Surface Plasmon Research
- Mitochondrial Function and Pathology
- Neuroscience and Neural Engineering
- Photoacoustic and Ultrasonic Imaging
- Photonic and Optical Devices
- Neuroinflammation and Neurodegeneration Mechanisms
- Optical Coherence Tomography Applications
- Epigenetics and DNA Methylation
- Advanced biosensing and bioanalysis techniques
- Cancer Cells and Metastasis
- Adipose Tissue and Metabolism
- Cell Image Analysis Techniques
- Cardiac Structural Anomalies and Repair
- Force Microscopy Techniques and Applications
- Planarian Biology and Electrostimulation
- Hippo pathway signaling and YAP/TAZ
University of Padua
2014-2025
Veneto Institute of Molecular Medicine
2012-2025
Città della Speranza Foundation
2018-2023
Consorzio per la Ricerca Sanitaria
2021-2023
The Francis Crick Institute
2020
Lundbeck (Italy)
2014
University of Milan
2014
MultiMedica
2014
University of Milano-Bicocca
2014
University of Palermo
2014
Rationale : The sympathetic nervous system plays a fundamental role in the regulation of myocardial function. During chronic pressure overload, overactivation induces release catecholamines, which activate β-adrenergic receptors cardiomyocytes and lead to increased heart rate cardiac contractility. However, stimulation leads impaired function, β-blockers are widely used as therapeutic agents for treatment disease. MicroRNA-133 (miR-133) is highly expressed myocardium involved controlling...
Bladder cancer (BC) is among the most common malignancies in world and a relevant cause of mortality. BC one frequent causes for bladder removal through radical cystectomy, gold-standard treatment localized muscle-invasive some cases high-risk, non-muscle-invasive cancer. In order to restore urinary functionality, an autologous intestinal segment has be used create diversion. However, several complications are associated with bowel-tract removal, affecting patients’ quality life. The present...
Extrasystoles lead to several consequences, ranging from uneventful palpitations lethal ventricular arrhythmias, in the presence of pathologies, such as myocardial ischemia. The role working versus conducting cardiomyocytes, well tissue requirements (minimal cell number) for generation extrasystoles, and properties leading ectopies become arrhythmia triggers (topology), normal diseased heart, have not been determined directly vivo. Here, we used optogenetics transgenic mice expressing...
Significance Mitochondrial calcium uniporter (MCU) is the core channel subunit of mitochondrial Ca 2+ complex (MCUC) and contributes to regulation ATP production. Here, we identify that adaptive maladaptive remodeling occurring in rodent human cardiomyocytes associated with changes MCU content, which are inversely correlated those its regulator microRNA-1 (miR-1). The present study thus defines molecular mechanism by content and, consequently, uptake directly modified alteration miR-1, a key...
Surface Plasmon Resonance (SPR)-based sensors have the advantage of being label-free, enzyme-free and real-time. However, their spreading in multidisciplinary research is still mostly limited to prism-coupled devices. Plasmonic gratings, combined with a simple cost-effective instrumentation, been poorly developed compared system mainly due lower sensitivity. Here we describe optimization signal enhancement sensing platform based on phase-interrogation method, which entails exploitation...
Tumor-associated macrophages (TAM) are regulators of extracellular matrix (ECM) remodeling and metastatic progression, the main cause cancer-associated death. We found that disabled homolog 2 mitogen-responsive phosphoprotein (DAB2) is highly expressed in tumor-infiltrating TAMs its genetic ablation significantly impairs lung metastasis formation. DAB2-expressing TAMs, mainly localized along tumor-invasive front, participate integrin recycling, ECM remodeling, directional migration a...
Xenogeneic pericardium-based substitutes are employed for several surgical indications after chemical shielding, limiting their biocompatibility and therapeutic durability. Adverse responses to these replacements might be prevented by tissue decellularization, ideally removing cells preserving the original extracellular matrix (ECM). The aim of this study was compare mostly applied pericardia in clinics, i.e., bovine porcine tissues, obtain new insights possible use. Bovine were submitted...
Label-Free Multiphoton Microscopy is a very powerful optical microscopy that can be applied to study samples with no need for exogenous fluorescent probes, keeping the main benefits of approach, like longer penetration depths and intrinsic sectioning, while opening possibility serial examinations different kinds techniques. Among many variations MPM, Higher Harmonic Generation (HHG) one most intriguing due its generally low photo-toxicity, which enables examination specimens particularly...
ABSTRACT Pathological angiogenesis is a hallmark of several conditions including eye diseases, inflammatory and cancer. Stromal cells play crucial role in regulating through the release soluble factors or direct contact with endothelial cells. Here, we analysed properties extracellular vesicles (EVs) released by bone marrow mesenchymal stromal (MSCs) explored possibility using them to therapeutically target angiogenesis. We demonstrated that response pro‐inflammatory cytokines, MSCs produce...
A cochlear implant (CI) is an electronic device that enables hearing recovery in patients with severe to profound loss. Although CIs are a successful treatment for impairment, their effectivity may be improved by reducing damages associated insertion of electrodes the cochlea, thus preserving residual ability. Inner ear trauma leads inflammatory reactions altering homeostasis and post-operative audiological performances electroacoustic stimulation. Strategies preserve ability led development...
The infrapatellar fat pad (IFP) is actively involved in knee osteoarthritis (OA). However, a proper description of which developmental modifications occur the IFP along with age and absence joint pathological conditions, required to adequately describe its actual contribution OA pathophysiology. Here, two sources were compared: (a) from healthy young patients undergoing anterior-cruciate ligament (ACL) reconstruction for ACL rupture (
Abstract Background Tumor hypoxia stimulates release of extracellular vesicles (EVs) that facilitate short- and long-range intercellular communication metastatization. Albeit EVs are known features Neuroblastoma (NB), a metastasis-prone childhood malignancy the sympathetic nervous system, whether hypoxic can NB dissemination is unclear. Methods Here we isolated characterized from normoxic cell culture supernatants performed microRNA (miRNA) cargo analysis to identify key mediators biological...
Regulation of mitochondrial Ca 2+ uptake is critical in cardiac adaptation to chronic stressors. Abnormalities handling, including mechanisms, have been implicated pathological heart hypertrophy. Enhancing uniporter (MCU) expression has suggested interfere with maladaptive development failure. Here, we addressed whether MCU modulation affects the response pressure overload. content was quantified human and murine hearts at different phases myocardial Cardiac function/structure were analyzed...
Mesenchymal stromal cells (MSCs) are key components of the tumor microenvironment (TME), influencing leukemia progression through poorly understood mechanisms. We investigated bioelectrical properties MSCs derived from pediatric AML patients (AML-MSCs) and identified a significant depolarization their resting membrane potential (Vmem, -14.7mV) compared to healthy (h-MSCs, -28.5mV), accompanied by downregulation CaV1.2 L-type calcium channel expression. AML-MSCs displayed increased...
The production of skeletal muscle constructs useful for replacing large defects in vivo, such as congenital diaphragmatic hernia (CDH), is still considered a challenge. standard application prosthetic material presents major limitations, recurrences remarkable number CDH patients. With this work, we developed tissue engineering approach based on decellularized and human cells the vitro generation diaphragmatic-like tissues proof-of-concept new option surgical treatment diaphragm defects. A...
Heart failure is the worst outcome of all cardiovascular diseases and still represents nowadays leading cause mortality with no effective clinical treatments, apart from organ transplantation allogeneic or artificial substitutes. Although applied as gold standard, heart cannot be considered a permanent answer because several drawbacks, side effects administered immunosuppressive therapies. For increasing number patients, biological cardiac substitute based on decellularized autologous cells...
Human and animal pericardia are among the most widely exploited materials suitable to repair damaged tissues in cardiovascular surgery context. Autologous, xenogeneic (chemically treated) homologous largely utilized, but they do exhibit some crucial drawbacks. Any tissue treated with glutaraldehyde is known be prone calcification vivo, lacks regeneration potential, has limited durability, can result cytotoxicity. Moreover, autologous have availability. Decellularized biological represent a...
Surface Plasmon Resonance (SPR) based sensors have the advantage of being label-free, enzyme-free and real-time. However, their spreading in multidisciplinary research is still limited almost confined to prism-coupled devices. Plasmonic gratings, combined with a simple cost-effective instrumentation, been poorly developed compared system mainly due lower sensitivity. Here we describe optimization signal enhancement sensing platform on phase-interrogation method, which entails exploitation...
The most common aortic valve diseases in adults are stenosis due to calcification and regurgitation. In pediatric patients, pathologies less common. When a native is surgically replaced by prosthetic one, it necessary consider that the latter has limited durability. particular, current bioprosthetic valves have be after approximately 10 years; mechanical prostheses more durable but require administration of permanent anticoagulant therapy. With regard both biological their inability follow...
In pediatric acute myeloid leukemia (AML), intensive chemotherapy and allogeneic hematopoietic stem cell transplantation are the cornerstones of treatment in high-risk cases, with severe late effects a still high risk disease recurrence as main drawbacks. The identification targeted, more effective, safer drugs is thus desirable. We performed high-throughput drug-screening assay 1280 compounds identified thioridazine (TDZ), drug that was highly selective for t(6;11)(q27;q23) MLL-AF6...
Adenosine A 2A receptors (A R) and dopamine D 2 (D are known to be involved in the physiological response hypoxia, their expression/activity may modulated by chronic sustained or intermittent hypoxia. To date, R can form transient physical receptor–receptor interactions (RRIs) giving rise a dynamic equilibrium able influence ligand binding signaling, as demonstrated different native tissues transfected mammalian cell systems. Given presence of type I cells, II afferent nerve terminals...