Abstract Plant-based milk contains high nutritional value with enriched vitamins, minerals, and essential amino acids. This study aimed to enhance the biochemical biological properties of rice through yeast fermentation, using novel fermenting strain Saccharomyces cerevisiae RSO4, which has superb ability for an innovative functional beverage. An integrated omics approach identified specific genes that exhibited genetic variants related various cellular processes, including flavor aroma...

The ability to rapidly respond nutrient changes is a fundamental requirement for cell survival. Here, we show that the zinc cluster regulator Znf1 responds altered signals following glucose starvation through direct control of genes involved in non-fermentative metabolism, including those belonged central pathways gluconeogenesis (PCK1, FBP1 and MDH2), glyoxylate shunt (MLS1 ICL1) tricarboxylic acid cycle (ACO1), which demonstrated by Znf1-binding enrichment at these promoters during...

The yeast S. cerevisiae is a major microbe that widely used in food and nonfood industries. However, accumulation of ethanol has negative effect on its growth limits production.

Microbial derived-surfactants display low eco-toxicity, diverse functionality, high biodegradability, specificity, and stability under extreme conditions. Sophorolipids are emerging as key biosurfactants of yeast origins, used in various industrial sectors to lower surface tension. Recently, sophorolipid complexes have been applied biomedicals agriculture eradicate infectious problems related human plant fungal pathogens. This study aimed characterize the functional properties antifungal...

In Saccharomyces cerevisiae, RDS2 encodes a zinc cluster transcription factor with unknown function. Here, we unravel key function of Rds2 in gluconeogenesis using chromatin immunoprecipitation-chip technology. While observed that binds to only few promoters glucose-containing medium, it many additional genes when the medium is shifted ethanol, nonfermentable carbon source. Interestingly, these are involved gluconeogenesis, tricarboxylic acid cycle, and glyoxylate cycle. Importantly, show...

Only trace amount of isobutanol is produced by the native Saccharomyces cerevisiae via degradation amino acids. Despite several attempts using engineered yeast strains expressing exogenous genes, catabolite repression glucose must be maintained together with high activity downstream enzymes, involving iron-sulfur assimilation and production. Here, we examined novel roles nonfermentable carbon transcription factor Znf1 in production during xylose utilization. RNA-seq analysis showed that...

Tualang honey is well known for its biological activities including as an antioxidant and anti-inflammatory. The compounds contributing to the are still unknown. Therefore, fractionation was carried out prepare phenolic-rich extract from using ethyl acetate (EA). EA could recover a wide range of phenolic obtained column chromatography (CC) liquid-liquid extraction (LLE) after acid hydrolysis. yield fraction LLE (0.83%) higher than that CC (0.39%). LLE-EA also contained more metabolites,...

Excessive use of antibiotics has detrimental consequences, including antibiotic resistance and gut microbiome destruction. Probiotic-rich diets help to restore good microbes, keeping the body healthy preventing onset chronic diseases. Honey contains not only prebiotic oligosaccharides but, like yogurt fermented foods, is an innovative natural source for probiotic discovery. Here, a collection three honeybee samples was screened yeast strains, aiming characterize their potential in vitro...

Biofilm-mediated drug resistance is a key virulence factor of pathogenic microbes that cause serious global health threat especially in immunocompromised individuals. Here, we investigated the antihyphal and antibiofilm activity 19,20‑epoxycytochalasin Q (ECQ), cytochalasin actin inhibitor isolated from medicinal mushroom Xylaria sp. BCC1067 against Candida albicans. Remarkably, 256 µg/ml ECQ inhibited over 95% C. albicans hyphal formation after 24 h-treatment. Combined lipid-based...

Efficient xylose alcoholic fermentation is one of the key to a successful lignocellulosic ethanol production. However, regulation this process in native xylose-fermenting yeasts poorly understood. In work, we paid attention transcriptional factor Cat8 and its possible role Ogataea (Hansenula) polymorpha. Saccharomyces cerevisiae, organism, which does not metabolize xylose, gene CAT8 encodes Zn-cluster activator necessary for expression genes involved gluconeogenesis, respiration, glyoxylic...

Abstract Benefits of whole grains as dietary supplements and active ingredients in health products have been promoted. Despite being neglected an agricultural byproduct polished rice, pigmented rice bran has emerged a promising source natural anti-aging compounds. Indeed, the extract red Hom Dang cultivar contained rich phenolic acids flavonoids. It displayed high antioxidant activities vitro vivo assays. Using yeast model, bioactive compounds, quercetin protocatechuic acid found pericarp,...

Abstract Saccharomyces cerevisiae offers an attractive platform for synthesis of biofuels and biochemical; however, robust strains that can withstand high substrate concentration fermentation conditions are required. To improve the yield productivity bioethanol, modification glucose metabolism cellular stress adaptation is investigated. Specifically, role Znf1 transcription factor in metabolic regulation characterized. Here, first shown to activate key genes glycolysis, pyruvate metabolism,...

The demand for the production of herbal extracts cosmetics, food, and health supplements, known as plant-based medicine, is rising globally. Incorporating could help to create higher value products due functional properties bioactive compounds. Because phytochemical composition vary depending on processing methods, a simple bioassay compounds an important screening method purposes characterization quality assurance. As simplified eukaryotic model, yeast serves versatile tool examine property...

Abstract To develop a cost‐effective microbial cell factory for the production of biofuels and biochemicals, an understanding tolerant mechanisms is vital construction robust host strains. Here, we characterized new function key metabolic transcription factor named Znf1 its involvement in stress response Saccharomyces cerevisiae to enhance tolerance advanced biofuel, isobutanol. RNA‐sequencing analysis wild‐type versus znf1 Δ deletion strains glucose revealed role pentose phosphate pathway...