A5008467812- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Microtubule and mitosis dynamics
- Digital Holography and Microscopy
- Optical Coherence Tomography Applications
- Cardiomyopathy and Myosin Studies
- Pancreatic and Hepatic Oncology Research
- Plant Micronutrient Interactions and Effects
- Spectroscopy Techniques in Biomedical and Chemical Research
- Plant Reproductive Biology
- Cellular Mechanics and Interactions
- Digital Radiography and Breast Imaging
- Hedgehog Signaling Pathway Studies
- Epigenetics and DNA Methylation
- Muscle Physiology and Disorders
- Plant tissue culture and regeneration
- Mitochondrial Function and Pathology
- Medical Imaging Techniques and Applications
- Metalloenzymes and iron-sulfur proteins
- Biomedical Research and Pathophysiology
- Photodynamic Therapy Research Studies
- Public Spaces through Art
- Geographies of human-animal interactions
- Salivary Gland Tumors Diagnosis and Treatment
- Genetic and Kidney Cyst Diseases
Cancer Research UK
2007-2019
University of Cambridge
2014-2019
Cancer Research UK Cambridge Center
2011-2018
Bexley Hall
2014
Ruhr University Bochum
2008
MRC Laboratory of Molecular Biology
1999-2004
Medical Research Council
2003
Royal Holloway University of London
1999
University of Bonn
1995
Pancreatic ductal adenocarcinoma (PDA) is among the most lethal human cancers in part because it insensitive to many chemotherapeutic drugs. Studying a mouse model of PDA that refractory clinically used drug gemcitabine, we found tumors this were poorly perfused and vascularized, properties are shared with PDA. We tested whether delivery efficacy gemcitabine mice could be improved by coadministration IPI-926, depletes tumor-associated stromal tissue inhibition Hedgehog cellular signaling...
Protein synthesis and autophagic degradation are regulated in an opposite manner by mammalian target of rapamycin (mTOR), whereas under certain conditions it would be beneficial if they occurred unison to handle rapid protein turnover. We observed a distinct cellular compartment at the trans side Golgi apparatus, TOR-autophagy spatial coupling (TASCC), where (auto)lysosomes mTOR accumulated during Ras-induced senescence. recruitment TASCC was amino acid- Rag guanosine...
Summary Myosins are a large superfamily of motor proteins which, in association with actin, involved intra‐ cellular motile processes. In addition to the conventional myosins muscle contractility, there is, animal cells, wide range unconventional implicated membrane‐associated processes, such as vesicle transport and membrane dynamics. plant however, very little is known about myosins. We have raised an antibody recombinant tail region Arabidopsis thaliana myosin 1 (a class VIII myosin) used...
Many copies of mammalian mitochondrial DNA contain a short triple-stranded region, or displacement loop (D-loop), in the major noncoding region. In 35 years since their discovery, no function has been assigned to D-loops. We purified nucleoprotein complexes from rat liver and identified previously uncharacterized protein, ATAD3p. Localization studies suggested that human ATAD3 is component many, but not all, nucleoids. Gene silencing by RNA interference altered structure nucleoids led...
Pollen tube growth is dependent on a dynamic actin cytoskeleton, suggesting that actin-regulating proteins are involved. We have examined the regulation of lily pollen-specific actin-depolymerizing factor (ADF) LlADF1. Its binding and depolymerizing activity pH sensitive, inhibited by certain phosphoinositides, but not controlled phosphorylation. Compared with its F-actin properties, low in depolymerization assays has been used to explain why pollen ADF decorates grains. This incompatible...
Structured illumination microscopy (SIM) has emerged as an essential technique for three-dimensional (3D) and live-cell super-resolution imaging. However, to date, there not been a dedicated workshop or journal issue covering the various aspects of SIM, from bespoke hardware software development use commercial instruments biological applications. This special aims recap recent developments well outline future trends. In addition we cover related topics such complementary techniques,...
Abstract Coherent anti‐Stokes Raman scattering (CARS) is becoming an established tool for label‐free multi‐photon imaging based on molecule specific vibrations in the sample. The technique has proven to be particularly useful lipids, which are abundant cells and tissues, including cytoplasmic lipid droplets (LD), recognized as dynamic organelles involved many cellular functions. increase number of undergoing cell proliferation a common feature neoplastic processes [1] LD also appears early...
Coherent anti-Stokes Raman scattering (CARS) has established itself as an imaging technique capable of providing video-rate biological specimens through vibrational coherence endogenous molecules. Current techniques predominantly involve the application costly, invasive and potentially non-specific dyes or labels for biomolecules. CARS microscopy can however provide a high-resolution non-invasive alternative biomolecules interest without need exogenous labels. Here we overview including...
Adipocytes are one of the major stromal cell components human breast. These cells play a key role in development gland and implicated breast tumorigenesis. Frequently, directional collagen I fibers found surrounding aggressive tumors. enhance cancer migration associated with poor patient prognosis. We sought to recapitulate these vitro provide three-dimensional (3D) model comprising adipose tissue anisotropic fibers. developed mesenchymal stem (hMSC) line capable undergoing differentiation...
Summary In this paper, differential phase imaging (DPC) with transmitted light is implemented by adding a suitable detection system to standard commercially available scanning confocal microscope. DPC, long‐established method in optical microscopy, depends on detecting the intensity difference between opposite halves or quadrants of split photodiode detector placed an aperture plane. Here, DPC compared scanned interference contrast (DIC) using variety biological specimens and objective...
All imaging modalities such as computed tomography (CT), emission and magnetic resonance (MRI) require a reconstruction approach to produce an image. A common image processing task for applications that utilise those is segmentation, typically performed posterior the reconstruction. We explore new combines segmentation in unified framework. derive variational model consists of total variation regularised from undersampled measurements Chan-Vese based segmentation. extend regularisation...
Although positional information, conveyed by morphogen gradients, is a widely accepted way of forming patterns during development, an alternative method conceivable, based on the intermingled differentiation cells with different fates, followed their sorting into discrete pattern elements. It has been proposed that Dictyostelium prestalk and prespore behave in this at mound stage development. However, it difficult to conclusively demonstrate they initially differentiate intermingled, because...
In this paper we propose a workflow to detect and track mitotic cells in time-lapse microscopy image sequences. order avoid the requirement for cell lines expressing fluorescent markers associated phototoxicity, phase contrast is often preferred over fluorescence live-cell imaging. However, common specific characteristics complicate processing impede use of standard methods. Nevertheless, automated analysis desirable due manual being subjective, biased extremely time-consuming large data...
The HIV-1 trans-activation responsive element (TAR) RNA stem-loop interacts with the HIV trans-activator protein Tat and other cellular factors to stimulate transcriptional elongation from viral long terminal repeat (LTR). Inhibitors of these interactions block full-length transcription and, hence, would potentially inhibit replication. We have studied structure-activity relationships in inhibition by steric 2′-O-methyl (OMe) oligonucleotides chimeras (mixmers) containing locked nucleic acid...
Shortly after its development, the white light supercontinuum laser was applied to confocal scanning microscopy as a more versatile substitute for multiple monochromatic lasers normally used excitation of fluorescence. This source is now available coupled commercial fluorescence microscopes. We have evaluated different purpose: interferometric imaging living cells and artificial models by interference reflection. in range 460-700 nm where this provides reasonably flat spectrum, obtained...
Abstract Long‐term benefits of cardiovascular therapeutic interventions are often reduced due to reocclusion the treated vessels. Therefore, a strategy reduce neointima formation would be great interest. A better understanding mechanisms underlying apoptosis in vascular smooth muscle cells key development such strategy. Efficient activation caspase‐8 regulated is largely dependent on aggregation FAS at cell surface, and method facilitate study clustering its role these particularly useful....
Abstract Background Huntingtin-interacting protein 1 (HIP1) is an adaptor involved in transcriptional regulation and receptor-mediated endocytosis. Overexpression of HIP1 transforms cell associated with, increasing grades prostate cancer (CaP) poor patient outcomes. However, the precise mechanism for role progression remains unknown. Methods Using a phospho-kinase antibody array we identified changes signalling with overexpression PNT1 cells. For validation Western blots were used together...