A5088005102- Antibiotic Resistance in Bacteria
- Salmonella and Campylobacter epidemiology
- SARS-CoV-2 and COVID-19 Research
- Streptococcal Infections and Treatments
- Neonatal and Maternal Infections
- RNA Interference and Gene Delivery
- Urinary Tract Infections Management
- Parvovirus B19 Infection Studies
- Amoebic Infections and Treatments
- Animal Virus Infections Studies
- Cytomegalovirus and herpesvirus research
- Antimicrobial Resistance in Staphylococcus
- Vector-Borne Animal Diseases
- Bacteriophages and microbial interactions
- Toxoplasma gondii Research Studies
- Reproductive tract infections research
- Gut microbiota and health
- Bladder and Urothelial Cancer Treatments
- Immunotherapy and Immune Responses
- Vector-borne infectious diseases
- Urinary Bladder and Prostate Research
- Antibiotics Pharmacokinetics and Efficacy
- Escherichia coli research studies
- Vibrio bacteria research studies
- Cambodian History and Society
Griffith University
2019-2024
Gold Coast Health
2021
Jeonbuk National University
2018
Kathmandu University
2012-2015
Central Queensland University
2015
Mahidol University
2012
Tribhuvan University
2009-2012
Siriraj Hospital
2012
East Tennessee State University
2011
College of Medical Sciences
2011
<i>Introduction:</i> Restless legs syndrome (RLS) is a common neurological disorder affecting 10% of the population. Most antidepressants exacerbate symptoms; however, correlational studies have noted symptom improvement with bupropion. The purpose current study was to examine whether, in controlled study, bupropion would improve symptoms RLS, or at least not them. <i>Methods:</i> This double-blinded, randomized trial. Twenty-nine participants moderate severe RLS received 150 mg...
The present study was performed to investigate the basic chemical composition and nutritional value of 10 terrestrial plant leaves (viz. Mangifera indica, Litchi chinensis, Artocarpus heterophyllus, Ziziphus mauritiana, Albizia lebbeck, Tamarindus Cicer arietinum, Bauhinia acuminata, Delonix regia Peltophorum pterocarpum ) abundant in tropical subtropical environments. leaf meals were evaluated comprehensively, encompassing proximate (% dry matter), amino acids, fatty antinutritional factors...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected more than 160 million people and resulted in 3.3 deaths, despite the availability of multiple vaccines, world still faces many challenges with their rollout. Here, we use high-density microarray patch (HD-MAP) to deliver a SARS-CoV-2 spike subunit vaccine directly skin. We show that is thermostable on patches, delivery enhancing both cellular antibody immune responses. Elicited antibodies potently neutralize clinically...
RNA interference (RNAi) is an emerging and promising therapy for a wide range of respiratory viral infections. This highly specific suppression can be achieved by the introduction short-interfering (siRNA) into mammalian systems, resulting in effective reduction load. Unfortunately, this has been hindered lack good delivery system, especially via intranasal (IN) route. Here, we have developed IN siRNA encapsulated lipid nanoparticle (LNP) vivo system that efficient at targeting severe acute...
Group B Streptococcus (GBS; also known as agalactiae) is an opportunistic bacterial pathogen that causes sepsis, meningitis, pneumonia, and skin soft tissue infections in neonates healthy or immunocompromised adults. GBS well-adapted to survive humans due a plethora of virulence mechanisms afford responses support survival dynamic host environments. These include counteraction cell death from exposure excess metal ions can cause mismetallation cytotoxicity, strategies combat molecules such...
Urinary tract infection (UTI) caused by uropathogenic Escherichia coli (UPEC) engages interleukin-10 (IL-10) as an early innate immune response to regulate inflammation and promote the control of bladder infection. However, mechanism engagement immunity UPEC that leads elicitation IL-10 in is unknown. Here, we identify major flagellar filament, FliC, a key bacterial component sensed system responsible for induction synthesis. responses human well mouse epithelial cell-monocyte cocultures...
The incidence of urinary tract infections (UTIs) is common in both diabetic and non patients. Since the microorganisms causing UTI vary their susceptibility to antimicrobials from place time time, hence constant screening trends pattern predominant organisms against essential. study aims determine spectrum ropathogens antibiotic sensitivity patients with clinically suspected .A prospective cross sectional was conducted during period February 2013 July among 90 attending Dhulikhel...
Urinary tract infections (UTI) frequently progress to chronicity in infected individuals but the mechanisms of pathogenesis underlying chronic UTI are not well understood. We examined role interleukin (IL)-17A because this cytokine promotes innate defense against uropathogenic Escherichia coli (UPEC). Analysis UPEC persistence and pyelonephritis mice deficient IL-17A revealed that CFT073 caused infection at a rate higher than multidrug resistant strain EC958. Il17a-/- exhibited with kidney...
Monitoring the antibiotic susceptibility pattern of Salmonella enterica serovar Typhi (S. Typhi) is important for efficiently managing cases typhoid fever. In this study, antimicrobial patterns 114 S. isolates, which were collected from a university hospital in Nepal during July 2009–December 2010, investigated by disc diffusion assays. All isolates sensitive to amoxycillin-clavulanic acid. More than 95% chloramphenicol, ceftazidime, ceftriaxone, and cotrimoxazole. addition, 1.7% studied...
Abstract SARS-CoV-2 has infected over 160 million people and resulted in more than 3.3 deaths, we still face many challenges the rollout of vaccines. Here, use high-density microarray patch to deliver a spike subunit vaccine directly skin. We show vaccine, dry-coated on is thermostable, delivery via HD-MAP induced greater cellular antibody immune responses, with serum able potently neutralize clinically relevant isolates including those from B.1.1.7 B.1.351 lineages. Finally, single dose...
Background Disc diffusion technique is the routine susceptibility testing procedure for isolates of enteric fever, most common clinical diagnosis among febrile patients in Nepal. Objective To evaluated current fluoroquinolones (FQs) criteria and nalidixic acid screening test Salmonella enterica serovar Typhi Paratyphi A. Methods S. A strains isolated from 443 suspected fever visiting National Public Health Laboratory (NPHL) during April through October 2008 were analyzed. All confirmed by...
INTRODUCTION: Enteric fever caused by Salmonella enterica serovars Typhi and Paratyphi A is the common clinical diagnosis among febrile patients presenting to hospital in Nepal. The aim of this study was evaluate ciprofloxacin (CIP) other antibiotics susceptibility patterns from blood samples suspected enteric visiting KIST Medical College, Kathmandu. MATERIALS AND METHODS: Antibiotic test performed Kirby Bauer disc diffusion method as per CLSI guidelines whereas MIC determination agar...
Streptococcus agalactiae, also known as group B Streptococcus, is an important human and animal pathogen. Zinc (Zn) essential trace element for normal bacterial physiology but intoxicates bacteria at high concentrations. Molecular systems Zn detoxification exist in S. however the degree to which may vary among different agalactiae isolates not clear. We measured resistance intoxication a diverse collection of clinical by comparing growth defined conditions stress. found significant...
The role of TORCH infections as a cause spontaneous abortions is still debatable with conflicting results where geographical variation may play significant role. This study was undertaken to discover the association in women by serological testing. descriptive case control conducted from January December, 2012. A evaluation carried out determine presence Toxoplasma gondii, Rubella, Cytomegalovirus (CMV) and Herpes Simplex Virus (HSV) specific immunoglobulin G (IgG) M (IgM) antibodies, using...
Long antisense RNAs (asRNAs) have been observed to repress HIV and other virus expression in a manner that is refractory viral evolution. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of disease 2019 (COVID-19) disease, has distinct ability evolve resistance around antibody targeting, as was evident from emergence various SARS-CoV-2 spike variants. Importantly, effectiveness current antivirals waning due rapid new variants concern, more recently omicron...
High altitude (HA) rangelands (e.g., grasslands, pastures, and shrublands) are important resources for the livelihoods of transhumance pastoralists. Transhumance pastoralists have developed used unique institutional practices using managing HA rangeland that better suit their local context. However, several issues emerged regarding use management such rangelands. Using secondary information our own experiences, we analyse resource tenure situation political ecology We found current these is...
Flagella are expressed on the surface of a wide range bacteria, conferring motility and contributing to virulence innate immune stimulation. Host-pathogen interaction studies roles flagella in infection, including due uropathogenic Escherichia coli (UPEC), have used various methods purify examine biology major subunit protein, FliC. These offered insight into ways which proteins interact with host cells. However, previous extract FliC, such as mechanical shearing, ultracentrifugation,...
Streptococcus agalactiae glyceraldehyde 3-phosphate dehydrogenase (GAPDH), encoded by gapC, is a glycolytic enzyme that associated with virulence and immune-mediated protection. However, the role of GAPDH in cellular cytokine responses to S. agalactiae, bacterial phagocytosis colonization female reproductive tract, central host niche, unknown. We expressed studied purified recombinant (rGAPDH) elicitation assays human monocyte-derived macrophage, epithelial cell, polymorphonuclear leukocyte...