A5061032454- Renal and related cancers
- Genetic and Kidney Cyst Diseases
- Tissue Engineering and Regenerative Medicine
- Renal cell carcinoma treatment
- Genetics, Aging, and Longevity in Model Organisms
- Cellular Mechanics and Interactions
- Pluripotent Stem Cells Research
- Hippo pathway signaling and YAP/TAZ
- Developmental Biology and Gene Regulation
- Congenital heart defects research
- Birth, Development, and Health
- Piezoelectric Actuators and Control
- Zebrafish Biomedical Research Applications
- Organ Donation and Transplantation
- Protist diversity and phylogeny
- Wnt/β-catenin signaling in development and cancer
- Spaceflight effects on biology
- Muscle Physiology and Disorders
- Neonatal Respiratory Health Research
The University of Texas Health Science Center at Houston
2016-2025
The University of Texas MD Anderson Cancer Center
2018-2025
RELX Group (Netherlands)
2023
RELX Group (United States)
2023
San Francisco State University
2009-2011
Objectives/Goals: This research aims to identify genetic alterations influencing congenital anomalies of the kidney and urinary tract (CAKUT) bridge a fundamental gap in understanding cellular mechanisms underlying development, with long-term goal enhancing treatments for renal anomalies. Methods/Study Population: We will use loss-of-function approach combination immunofluorescent microscopy techniques determine influence Dnmbp perturbation on Daam1 localization, actin assembly, junctional...
The embryonic kidney of Xenopus laevis (frog), the pronephros, consists a single nephron, and can be used as model for disease. embryos are large, develop externally, easily manipulated by microinjection or surgical procedures. In addition, fate maps have been established early embryos. Targeted into individual blastomere that will eventually give rise to an organ tissue interest selectively overexpress knock down gene expression within this restricted region, decreasing secondary effects in...
E-cadherin junctions facilitate assembly and disassembly of cell contacts that drive development homeostasis epithelial tissues. In this study, using Xenopus embryonic kidney Madin-Darby canine (MDCK) cells, we investigate the role Wnt/planar polarity (PCP) formin Daam1 (Dishevelled-associated activator morphogenesis 1) in regulating E-cadherin-based intercellular adhesion. Using live imaging, show localizes to newly formed developing nephron. Furthermore, analyses junctional filamentous...
Xenopus laevis embryos are an established model for studying kidney development. The nephron structure and genetic pathways that regulate nephrogenesis conserved between humans, allowing the study of human disease-causing genes. also amenable to large-scale screening, but studies disease-related genes have been impeded because assessment development has largely limited examining fixed embryos. To overcome this problem, we generated a transgenic line labels kidney. We characterize cdh17:eGFP...
Kidneys are composed of numerous ciliated epithelial tubules called nephrons. Each nephron functions to reabsorb nutrients and concentrate waste products into urine. Defects in primary cilia associated with abnormal formation nephrons cyst a wide range kidney disorders. Previous work Xenopus laevis zebrafish embryos established that loss components make up the Wnt/PCP pathway, Daam1 ArhGEF19 (wGEF) perturb tubulogenesis. Dishevelled, which activates both canonical non-canonical affect...
Abstract Somites give rise to the vertebral column and segmented musculature of adult vertebrates. The cell movements that position cells within somites along anteroposterior dorsoventral axes are not well understood. Using a fate mapping approach, we show at onset Xenopus laevis gastrulation, mesoderm undergo distinct form myotome fibers positioned in discrete locations axis. We distribution presomitic axis is influenced by convergent extension notochord. Heterochronic heterotopic...
Abstract The kidney is an essential organ that ensures bodily fluid homeostasis and removes soluble waste products from the organism. functional units within kidneys are epithelial tubules called nephrons. These take in filtrate blood or coelom selectively reabsorb nutrients through evolutionarily conserved nephron segments, leaving product to be eliminated urine. Genes coding for transporters segmentally expressed, enabling nephrons function as selective filters. developmental patterning...
The embryonic kidney of Xenopus laevis (frog), the pronephros, consists a single nephron, and can be used as model for disease. embryos are large, develop externally, easily manipulated by microinjection or surgical procedures. In addition, fate maps have been established early embryos. Targeted into individual blastomere that will eventually give rise to an organ tissue interest selectively overexpress knock down gene expression within this restricted region, decreasing secondary effects in...
ABSTRACT Kidneys are composed of numerous ciliated epithelial tubules called nephrons. Each nephron functions to reabsorb nutrients and concentrate waste products into urine. Defects in primary cilia associated with abnormal formation nephrons cyst a wide range kidney disorders. Previous work Xenopus laevis zebrafish embryos established that loss components make up the Wnt/PCP pathway, Daam1 ArhGEF19 (wGEF) perturb tubulogenesis. Dishevelled, which activates both canonical non-canonical...
SUMMARY E-cadherin junctions facilitate the assembly and disassembly of cell-cell contacts that drive development homeostasis epithelial tissues. The stability E-cadherin-based highly depends on their attachment to actin cytoskeleton, but little is known about how junctional filaments regulated. Formins are a conserved group proteins responsible for formation elongation filamentous (F-actin). In this study, using Xenopus embryonic kidney Madin-Darby canine (MDCK) cells, we investigate role...
Abstract An image of an X. laevis tadpole showing rhodamine‐labeled transplanted presomitic mesoderm cells (red) in which a subset have differentiated into myotome fibers (shown orange). The are stained with the antibody 12/101 (green) and notochord is Tor 70 (blue). was digitally duplicated twice fused at anterior end. From Krneta‐Stankic et al., Developmental Dynamics 239:1162–1177, 2010.