ABSTRACT Staphylococcal cassette chromosome mec (SCC ) typing is essential for understanding the molecular epidemiology of methicillin-resistant Staphylococcus aureus (MRSA). SCC elements are currently classified into types I to V based on nature and ccr gene complexes, further subtypes according their junkyard region DNA segments. Previously described traditional PCR schemes require multiple primer sets experiments, while a previously published multiplex assay limited in its ability detect...

Antibiotics such as erythromycin and rifampicin, at low concentrations, alter global bacterial transcription patterns measured by the stimulation or inhibition of a variety promoter– lux reporter constructs in Salmonella typhimurium library. Analysis 6,500-clone library indicated that many 5% promoters may be affected, comprising genes for functions, well significant fraction with no known function. Studies selection clones showed varied depending on nature antibiotic, promoter, what culture...

ABSTRACT We developed a new multiplex PCR assay for detection of Panton-Valentine leukocidin virulence genes and simultaneous discrimination methicillin-susceptible from -resistant staphylococci. This is simple, rapid, accurate offers the potential prompt newly emerging community-associated methicillin-resistant Staphylococcus aureus .

ABSTRACT Metallo-β-lactamases (MBLs) have been increasingly recognized from clinical isolates worldwide, but the laboratory detection of these strains is not well defined. We report a study that developed an EDTA disk screen test and molecular diagnostic assay for MBL-producing Pseudomonas aeruginosa . Using NCCLS methodology, inhibition zone diameters were determined in tests with imipenem (IPM) meropenem (MEM) disks alone combination 930 μg EDTA. This was compared MBL Etest. The duplex PCR...

The recent advances in large-scale monitoring of gene expression raise the challenge mapping systems on basis kinetic data living cells. To address this, we measured promoter activity flagellar system Escherichia coli at high accuracy and temporal resolution by means reporter plasmids. genes pathway were ordered analysis algorithms without dependence mutant strains. observed program transcription was much more detailed than previously thought associated with multiple steps flagella assembly.

ABSTRACT Staphylococcal cassette chromosome mec (SCC ) is a mobile genetic element characterized by flanking terminal direct and, in most cases, inverted repeat sequences, the and ccr gene complexes, their surrounding DNA regions. Unique combinations of complexes generate various SCC types. Six types have been reported to date. We describe here novel type identified Canadian methicillin-resistant Staphylococcus aureus (MRSA) epidemic strain. MRSA clinical isolates were screened for known...

We developed a novel multiplex PCR assay for rapid identification and discrimination of the USA300 USA400 strains concomitant detection Panton-Valentine leukocidin genes, with simultaneous methicillin-resistant Staphylococcus aureus from methicillin-susceptible S. strains, coagulase-negative staphylococci, staphylococci other bacteria.

Plasmid profiles, phage typing, antibiograms, and biotyping were used to characterize Staphylococcus epidermidis isolated from multiple cultures of blood four patients with prosthetic valve endocarditis. Epidemiological evidence implicated a common source for these infections. Of 20 clinically significant isolates, 14 exhibited variations the prototype pattern resistance five antibiotics. All isolates tested appeared be same strain by typing. 18 available plasmid analysis, 10 contained six...

Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) strains often carry the Panton-Valentine leukocidin (PVL) genes. However, specific role that PVL plays in epidemiological features and pathogenesis of CA-MRSA infections has remained undefined controversial. Conducting a retrospective study on natural population MRSA clinical isolates recovered from community hospital patients large Canadian health-care region during 6-year period, we identified coexistence 2 USA400...

An increasing number of severe infections caused by Staphylococcus aureus ST398 strains has been observed. However, it not elucidated whether all are equally virulent. We collected 13 from China and Canada to test in a Caenorhabditis elegans infection model compared their whole genome sequences (WGS) explore potential insights into virulence. All isolates belonged ST398-methicillin-susceptible S. (MSSA) with variant spa types (t034, t571, t1451, t1250). Pulsed field gel electrophoresis...

The timely detection of Methicillin-resistant Staphylococcus aureus (MRSA) is crucial for antimicrobial therapy and a key factor to limit the hospital spread MRSA. Currently available commercial MRSA assays target 3' end orfX gene right extremity Staphylococcal Cassette Chromosome mec (SCCmec). These suffer from both false positive due SCC-like elements that lack mecA negative results inability detect new or variant SCCmec cassettes with existing primers. We developed novel scheme, designed...

ABSTRACT Methicillin-resistant Staphylococcus aureus (MRSA) is a clinically significant pathogen that resistant to wide variety of antibiotics and responsible for large number nosocomial infections worldwide. The Agency Healthcare Research Quality the Centers Disease Control Prevention recently recommended adoption universal mupirocin-chlorhexidine decolonization all admitted intensive care unit patients rather than MRSA screening with targeted treatments, which raises serious concern about...

USA300 is a predominant community-associated methicillin-resistant Staphylococcus aureus strain which carries an arginine catabolic mobile element (ACME). ACME contains potential virulence factors including deiminase (arc) pathway and oligopeptide permease (opp-3) system, are proposed to play role in bacterial transmission. However, the of evolution pathogenicity remains be elucidated. arcA deletion mutants were created by allelic replacement from clinical isolate. By comparing wild type...

While numerous studies examine the epidemiology and molecular characterization of Staphylococcus aureus in most developed countries, detailed S. strains clones Africa is lacking. We determined virulence 81 non-duplicate isolates from Benin-City, Nigeria, collected during January–July 2016, compared with global strains. Forty-seven (58.0%) were found to be methicillin-sensitive (MSSA), while 34 (42.0%) methicillin-resistant (MRSA). ST152-MSSA (24.7%) ST7-MRSA-V (19.8%) dominant groups...

Staphylococcus aureus sequence type (ST) 398 is a lineage affecting both humans and livestock worldwide. However, the mechanisms underlying its clonal evolution are still not clearly elucidated. We applied whole-genome sequencing (WGS) typing to 45 S. strains from China Canada between 2005 2014, in order gain insight into their evolutionary pathway. Based on WGS phylogenetic analysis, 42 isolates were assigned human-associated clade (I/II-GOI) 3 livestock-associated (IIa). Phylogeny of ϕSa3...

Staphylococcal Cassette Chromosome mec (SCCmec) typing is a very important molecular tool for understanding the epidemiology and clonal strain relatedness of methicillin-resistant Staphylococcus aureus (MRSA), particularly with emerging outbreaks community-associated MRSA (CA-MRSA) occurring on worldwide basis. Traditional PCR schemes classify SCCmec by targeting identifying individual ccr gene complex types, but require use many primer sets multiple experiments. We designed published simple...

Despite initially being described in North America, Staphylococcus aureus (SA) sequence type ST59 is the most commonly isolated Eastern Asia. The origins and evolution of this strain remains unclear therefore we gathered a collection isolates from Canada mainland China for detailed genetic analysis lineage. Bayesian inference phylogenomic our isolates, along with previously published sequences indicated that lineage could be divided into 6 distinct subgroups (WGS-1 thorough 6), each having...

Buffalo represent a major source of milk in Pakistan. However, production is impacted by the disease bovine mastitis. Mastitis causes significant economic losses, with Staphylococcus aureus (S. aureus) being one its causative agents. While much work has been done understanding epidemiology mastitis Pakistan, detailed molecular characterization associated S. unavailable. In current study both epidemiological and from Hazara division Pakistan are examined. was isolated 18.41% animals, left...

The genomic comparison of virulent (TW20), moderately (CMRSA6/CMRSA3) and avirulent (M92) strains from a genetically closely-related MRSA ST239 sub-lineage revealed striking similarities in their genomes antibiotic resistance profiles, despite differences virulence pathogenicity. main were the spa gene (coding for staphylococcal protein A), lpl genes lipoprotein-like membrane proteins), cta (genes involved heme synthesis), dfrG trimethoprim-resistant dihydrofolate reductase), as well...

Methicillin-resistant Staphylococcus aureus (MRSA) strains from different geographic areas have genetic backgrounds, suggesting independent clonal evolutions. To better understand the virulence of MRSA and relationship to their origins, we undertook an analysis epidemiologic, molecular, characteristics a large number isolates geographically diverse in Caenorhabditis elegans infection model. A total 99 collected between 1993 2010 at Geneva University Hospitals global origins were...