A5074707329- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Retinal Development and Disorders
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Virus-based gene therapy research
- Corneal Surgery and Treatments
- Dendrimers and Hyperbranched Polymers
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Retinal Diseases and Treatments
- RNA Research and Splicing
- Molecular Biology Techniques and Applications
- Nanoplatforms for cancer theranostics
- PI3K/AKT/mTOR signaling in cancer
- Bone Tissue Engineering Materials
- Circular RNAs in diseases
- Prostate Cancer Treatment and Research
- Bone and Dental Protein Studies
- Bladder and Urothelial Cancer Treatments
- Zebrafish Biomedical Research Applications
- Beetle Biology and Toxicology Studies
- Liver physiology and pathology
- Advanced Drug Delivery Systems
- Ocular Disorders and Treatments
Case Western Reserve University
2015-2025
Zunyi Medical University
2025
Wenzhou University
2024
BGI Group (China)
2015-2016
Cleveland Clinic
2015
Nanjing University
2015
Nanjing University of Chinese Medicine
2014
Jiangsu Cancer Hospital
2014
Nanjing Medical University
2014
Tianjin University
2011
Long noncoding RNAs (lncRNAs), by virtue of their versatility and multilevel gene regulation, have emerged as attractive pharmacological targets for treating heterogeneous complex malignancies like triple-negative breast cancer (TNBC). Despite multiple studies on lncRNA functions in tumor pathology, systemic targeting these "undruggable" macromolecules with conventional approaches remains a challenge. Here, we demonstrate effective TNBC therapy nanoparticle-mediated RNAi the oncogenic DANCR,...
Extradomain-B fibronectin (EDB-FN), one of the oncofetal (onfFN) isoforms, is a high-molecular-weight glycoprotein that mediates cell adhesion and migration. The expression EDB-FN associated with number cancer-related biological processes such as tumorigenesis, angiogenesis, epithelial-to-mesenchymal transition (EMT). Here, we report development small peptide specific to for targeting prostate cancer. A cyclic nonapeptide, CTVRTSADC (ZD2), was identified using phage display. ZD2-Cy5...
Evidences have shown that the RAS signalling pathway plays an important role in colorectal cancer (CRC). Moreover, RAS-GTPase-activating proteins (RASGAPs) as terminators are associated with tumourigenicity and tumour progression. In this study, we used bioinformatics analysis to predict study miRNAs could target p21 GTPase-activating protein 1 (RASA1), member of RASGAPs.The levels RASA1 miR-223 were analysed by real-time PCR, western blotting or situ immunofluorescence analyses. The...
Abstract The phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) pathway has been identified as an important in renal cell carcinoma (RCC). We have reported a nonsense mutation PIK3R1 , which encodes the regulatory subunit of PI3K, metastatic RCC (mRCC), while was absent corresponding primary (pRCC). To identify function RCC, we examined its expression normal kidney, pRCC and mRCC by immunohistochemistry real-time polymerase chain reaction. significantly decreased further reduced...
Background: Multidrug resistance (MDR) directly contributes to the clinical failure of chemotherapy in breast cancer (BCA). β-elemene is a natural antitumor drug from plants. We previously confirmed that MDR could be reversed by β-elemene. In this study, we intended investigate reversal effect on human BCA adriacin (Adr) -resistant MCF-7 cells (MCF-7/Adr) and docetaxel (Doc) - resistant (MCF-7/Doc) through gene regulatory network. Methods: MTT-cytotoxic, miRNA microarray, Real-time...
Differentiation antagonizing noncoding RNA (DANCR) is recognized as an oncogenic long (lncRNA) overexpressed in triple negative breast cancer (TNBC). We showed a previous study that RNAi with targeted multifunctional ionizable lipid ECO/siRNA nanoparticles was effective to regulate this undruggable target for treatment of TNBC. In study, we developed dual-targeted ECO/siDANCR by targeting tumor extracellular matrix oncoprotein, extradomain B fibronectin (EDB-FN), and integrins on cells...
Stargardt disease (STGD) is an autosomal recessive retinal disorder caused by a monogenic ABCA4 mutation. Currently, there no effective therapy to cure disease. The replacement of mutated with functional gene remains attractive strategy. In this study, we have developed non-viral using nanoparticles self-assembled multifunctional pH-sensitive amino lipid ECO and therapeutic plasmid. mediated efficient intracellular transduction in wild-type (WT) Abca4−/− mice. Specific expression the outer...
CRISPR/Cas9 system is a promising approach for gene editing in therapy. Effective requires safe and efficient delivery of target cells. Several new multifunctional pH-sensitive amino lipids were designed synthesized with modification the head groups intracellular system. These exhibited structurally dependent formulation stable nanoparticles DNA plasmids sizes ranging from 100 to 200 nm. The lipid plasmid showed hemolysis minimal hemolytic activity at pH 7.4 increased acidic (pH = 5.5, 6.5)....
Stargardt disease (STGD) is the most common form of inherited retinal genetic disorders and often caused by mutations in ABCA4. Gene therapy has promise to effectively treat monogenic disorders. However, clinically approved adeno-associated virus (AAV) vectors do not have a loading capacity for large genes, such as Self-assembly nanoparticles composed (1-aminoethyl)iminobis[N-(oleoylcysteinyl-1-amino-ethyl)propionamide (ECO; multifunctional pH-sensitive/ionizable amino lipid) plasmid DNA...
The unfolded protein response (UPR) drives events that promote diabetic retinopathy including VEGF upregulation in Müller cells. How UPR is activated vivo the retina not well understood. CD40 required for development of but whether mediates activation sensors unknown. ligation cells caused phospholipase Cγ1 (PLCγ1)-dependent (PERK, IRE1α and ATF6α), production dependent on PLCγ1 sensors. Diabetic Cd40-/- mice did exhibit retina. These responses were restored rescued to express WT a mutant...
The aim of this study was to investigate whether magnesium cantharidate (MC) exerts anti-hepatocellular carcinoma (anti-HCC) effects by targeting receptor for activated C kinase 1 (RACK1). Cancer Genome Atlas (TCGA) database used analyze the expression RACK1 in liver tissues. Molecular docking examine binding interactions between MC and RACK1. Huh-7 SK-Hep-1 cancer cells' viability, proliferation, apoptosis were assessed using Cell Counting Kit-8 (CCK-8) assay, 5-ethynyl-2'-deoxyuridine...
Abstract Development of safe and effective gene delivery systems is essential in treating ocular genetic disorders. A hybrid nonviral system composed a multifunctional lipid ECO G4 nanoglobule was designed for efficient into RPE cells at low charge ratios. This formed stable DNA nanoparticles N/P ratios, exhibited cytotoxicity, induced higher GFP expression ARPE‐19 = 6. The mediated significant reporter ex‐vivo the retina from wild type C57 mice vivo BALB/c mice. These are promising vitro
Nanoparticle based siRNA formulations often suffer from aggregation and loss of function during storage. We in this study report a frozen targeted RGD-polyethylene glycol (PEG)-ECO/siβ3 nanoparticle formulation with prolonged shelf life preserved functionality. The RGD-PEG-ECO/siβ3 nanoparticles are formed by step-wised self-assembly RGD-PEG-maleimide, ECO, siRNA. have diameter 224.5 ± 9.41 nm zeta potential to 45.96 3.67 mV water size 234.34 3.01 near neutral saline solution. addition...
Long noncoding RNA (lncRNA) differentiation antagonizing (DANCR) is overexpressed in human triple-negative breast cancer (TNBC) and promotes cell migration proliferation. TNBC limited treatment options relative to hormone-receptor-positive commonly treated with chemotherapy, which often compromised by acquired resistance. DANCR has been implicated the development of chemoresistance across multiple types. Here, we applied magnetic resonance molecular imaging (MRMI) a targeted contrast agent,...
Biocompatible dendrimers with well-defined nanosizes are increasingly being used as carriers for drug delivery. 5-Aminosalicylic acid (5-ASA) is an FDA-approved therapeutic agent recently found effective in treating retinal degeneration of animal models. Here, a water-soluble dendrimer conjugate 5-ASA (AGFB-ASA) was designed to treat such degeneration. The conjugated generation 2 (G2) lysine silsesquioxane core (nanoglobule) by using hydrolyzable Schiff base spacer. Incubation nanoglobular...
// Song Wu 1,4,* , Zhao Yang 2,3,* Rui Ye 5,* Dan An Chong Li 2,* Yitian Wang 4,6,* Yongqiang 7 Yi Huang 4 Huan Liu 5 Feida Luyun He 2 Da Sun Yuan Yu Qiaoling Peide Meng Zhang 6 Xin Tengteng Bi Xuehan Zhuang Liyan Jingxiao Lu Xiaojuan Fangjian Zhou Chunxiao 8 Guosheng 9 Yong Hou Zusen Fan and Zhiming Cai 4,6 1 The Affiliated Luohu Hospital of Shenzhen University, Group, Shenzhen, China CAS Key Laboratory Infection Immunity, Institute Biophysics, Chinese Academy Sciences, Beijing, 3...
ABSTRACT Few layer graphene (FLG) has been used to improve different properties of polymers. FLG‐modified waterborne epoxy dispersions were prepared using a hydrophilic oligomer (HEO‐144) as the surfactant, which was liquid resin and poly(ethylene glycol) (PEG4000) at molar ratio 1.0. To enhance stability dispersions, fumed silica added best reached content 1.5%. Thermogravimetric analyses demonstrated enhanced thermal films after FLG modification. With 1.0%, T 5% modified increased 332 °C...
Dysfunctions caused by gene mutations in the retinal pigment epithelium (RPE) lead to degeneration, visual function loss, and even blindness. RPE-specific replacement therapy holds great promise for treating monogenic ocular disorders RPE. Although adeno-associated virus (AAV) has been approved treat disorders, broad clinical applications of AAV-based are limited its loading capacity. In this work, we intended design develop a stable retinylamine analogue ACU4429-modified dual pH-sensitive...