A5036736067- Blood properties and coagulation
- Force Microscopy Techniques and Applications
- Electrospun Nanofibers in Biomedical Applications
- Cellular Mechanics and Interactions
- DNA and Nucleic Acid Chemistry
- Mechanical and Optical Resonators
- Advanced Sensor and Energy Harvesting Materials
- Erythrocyte Function and Pathophysiology
- Advanced biosensing and bioanalysis techniques
- Near-Field Optical Microscopy
- 3D Printing in Biomedical Research
- Carbon Nanotubes in Composites
- Microfluidic and Bio-sensing Technologies
- Molecular Junctions and Nanostructures
- RNA and protein synthesis mechanisms
- Conducting polymers and applications
- Diabetic Foot Ulcer Assessment and Management
- Tendon Structure and Treatment
- Bacteriophages and microbial interactions
- Silk-based biomaterials and applications
- Platelet Disorders and Treatments
- Genetic factors in colorectal cancer
- Bacterial Genetics and Biotechnology
- Perovskite Materials and Applications
- Cell Adhesion Molecules Research
Wake Forest University
2015-2024
Atrium Health Wake Forest Baptist
2009
NanoMedica (United States)
2007
University of North Carolina at Chapel Hill
1999-2006
Harvard University
2006
University of Oregon
1992-1999
North Carolina State University
1999
University of California, Santa Barbara
1997
National Cancer Institute
1997
University of Rochester
1997
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCircular DNA molecules imaged in air by scanning force microscopyCarlos Bustamante, James Vesenka, Chun Lin Tang, William Rees, Martin Guthold, and Rebecca KellerCite this: Biochemistry 1992, 31, 1, 22–26Publication Date (Print):January 14, 1992Publication History Published online1 May 2002Published inissue 14 January 1992https://doi.org/10.1021/bi00116a005RIGHTS & PERMISSIONSArticle Views988Altmetric-Citations329LEARN ABOUT THESE METRICSArticle...
Due to its low cost, biocompatibility and slow bioresorption, poly-ε-caprolactone (PCL) continues be a suitable material for select biomedical engineering applications. We used combined atomic force microscopy (AFM)/optical technique determine key mechanical properties of individual electrospun PCL nanofibers with diameters between 440-1040nm. Compared protein nanofibers, showed much lower adhesion, as they slipped on the substrate when mechanically manipulated. We, therefore, first...
Biomaterials based on electrospun nanofibers are increasingly used in biomedical applications. Polycaprolactone (PCL) is a biocompatible, biodegradable, FDA-approved, synthetic polymer suitable for such In this study, we nanomanipulation technique combined atomic force/optical microscope to investigate key mechanical properties of PCL as function molecular weight (150 kDa, 114 and 74 kDa) fiber diameter (20–250 nm). Mechanical extracted from different types stress-strain curves included...
Fluid tapping-mode atomic force microscopy (AFM) was used to observe Escherichia coli RNA polymerase (RNAP) transcribing two different linear double-stranded (ds) DNA templates. The transcription process detected by observing the translocation of template RNAP on addition ribonucleoside 5'-triphosphates (NTPs) in sequential AFM images. Stalled ternary complexes RNAP, dsDNA and nascent were adsorbed onto a mica surface imaged under continuously flowing buffer. On introduction all four NTPs,...
Blood clots perform an essential mechanical task, yet the behavior of fibrin fibers, which form structural framework a clot, is largely unknown. By using combined atomic force-fluorescence microscopy, we determined elastic limit and extensibility individual fibers. Fibrin fibers can be strained 180% (2.8-fold extension) without sustaining permanent lengthening, they up to 525% (average 330%) before rupturing. This largest observed for protein The data imply that monomers must able undergo...
Nanoparticles, including multiwalled carbon nanotubes (MWNTs), strongly absorb near-infrared (nIR) radiation and efficiently convert absorbed energy to released heat which can be used for localized hyperthermia applications. We demonstrate the first time that DNA-encasement increases emission following nIR irradiation of MWNTs, DNA-encased MWNTs safely eradicate a tumor mass in vivo. Upon is generated with linear dependence on laser power. resulted 3-fold reduction concentration required...
We present here the use of amino-terminated DNA strands in functionalizing open ends and defect sites oxidatively prepared single-walled carbon nanotubes, an important first step realizing a DNA-guided self-assembly process for nanotubes.
The nanoManipulator system adds a virtual-reality interface to an atomic-force microscope (AFM), thus providing tool that can be used by scientists image and manipulate nanometer-sized molecular structures in controlled manner. As the AFM tip scans sample, tip-sample interaction forces are monitored, which, turn, yield information about frictional, mechanical, material, topological properties of sample. Computer graphics reconstruct surface for user, with color or contours overlaid indicate...
The major structural component of a blood clot is mesh fibrin fibers. Our goal was to determine whether fibrinogen glycation and fiber diameter have an effect on the mechanical properties single We used combined atomic force microscopy/fluorescence microscopy technique individual fibers formed from plasma. Blood samples were taken uncontrolled diabetic patients as well age-, gender-, body-mass-index-matched healthy individuals. then underwent treatment control glucose levels before end...
Efficient injection of charge carriers from the contacts into semiconductor layer is crucial for achieving high-performance organic devices. The potential drop necessary to accomplish this process yields a resistance associated with contacts, namely contact resistance. A large can limit operation devices and even lead inaccuracies in extraction device parameters. Here, we demonstrate simple efficient strategy reducing thin-film transistors by more than an order magnitude creating high work...
The capability of the scanning force microscope (SFM) to image molecules in aqueous buffers has opened exciting possibility following processes molecular assembly real time and near-physiological environments. This is demonstrated this paper by process RNA polymerase-DNA complexes. DNA fragments deposited on mica imaged Hepes/MgCl2 are shown before after Escherichia coli polymerase holoenzyme injected SFM liquid chamber. protein can recognize bind these within several seconds injection,...
The electrical properties of organic field-effect transistors are governed by the quality constituting layers, and resulting interfaces. We compare same semiconductor film, 2,8-difluoro- 5,11-bis (triethylsilylethynyl) anthradithiophene, with bottom SiO2 dielectric top Cytop find a 10× increase in charge carrier mobility, from 0.17 ± 0.19 cm2 V−1 s−1 to 1.5 0.70 s−1, when polymer is used. This results significant reduction trap density states band-gap, decrease contact resistance.
We have designed and tested a new, inexpensive, easy-to-make easy-to-use calibration standard for atomic force microscopy (AFM) lateral measurements. This new simply consists of small glass fiber known dimensions Young’s modulus, which is fixed at one end to substrate can be bent laterally with the AFM tip other end. has equal or less error than commonly used method using beam mechanics determine cantilever’s constant. It transferable, thus providing universal tool comparing calibrations...
At many promoters, transcription is regulated by simultaneous binding of a protein to multiple sites on DNA, but the structures and dynamics such factor-mediated DNA loops are poorly understood. We directly examined in vitro loop formation mediated Escherichia coli lactose repressor using single-molecule structural kinetics methods. Small ( approximately 150 bp) form quickly stably, even with out-of-phase operator spacings. Unexpectedly, repeated spontaneous transitions between two distinct...