A5020876205- Lipid metabolism and biosynthesis
- Endoplasmic Reticulum Stress and Disease
- Ubiquitin and proteasome pathways
- Cellular transport and secretion
- Peroxisome Proliferator-Activated Receptors
- Pancreatic function and diabetes
- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- Advanced Optical Sensing Technologies
- RNA modifications and cancer
- Lysosomal Storage Disorders Research
- Moyamoya disease diagnosis and treatment
- Microbial Metabolic Engineering and Bioproduction
- Cell Image Analysis Techniques
- RNA and protein synthesis mechanisms
- Advanced Fluorescence Microscopy Techniques
- Adipose Tissue and Metabolism
- Parkinson's Disease Mechanisms and Treatments
Yale University
2022
Harvard University
2018-2020
Howard Hughes Medical Institute
2018-2020
Broad Institute
2020
The endoplasmic reticulum is a cellular hub of lipid metabolism, coordinating synthesis with continuous changes in metabolic flux. Maintaining ER homeostasis despite these fluctuations crucial to cell function and viability. Here, we identify novel mechanism that for normal metabolism protects the from dysfunction. We molecular evolutionarily conserved protein FIT2 as fatty acyl–coenzyme A (CoA) diphosphatase hydrolyzes acyl–CoA yield acyl 4′-phosphopantetheine. This activity FIT2, which...
Numerous mutations that impair retrograde membrane trafficking between endosomes and the Golgi apparatus lead to neurodegenerative diseases. For example, in endosomal retromer complex are implicated Alzheimer’s Parkinson’s diseases, of Golgi-associated protein (GARP) cause progressive cerebello-cerebral atrophy type 2 (PCCA2). However, how these neurodegeneration is unknown. GARP yeast, including one causing PCCA2, result sphingolipid abnormalities impaired cell growth corrected by treatment...
SUMMARY The endoplasmic reticulum (ER) protein Fat-Induced Transcript 2 (FIT2) has emerged as a key factor in lipid droplet (LD) formation, although its molecular function is unknown. Highlighting importance, FIT2 orthologs are essential worms and mice, deficiency causes deafness/dystonia syndrome humans. Here we show that phosphate phosphatase (LPP) enzyme required for maintaining the normal structure of ER. Recombinant exhibits LPP activity vitro loss this cells leads to ER membrane...
Genome editing technologies introduce targeted chromosomal modifications in organisms yet are constrained by the inability to selectively modify repetitive genetic elements. Here we describe filtered editing, a genome method that embeds group 1 self-splicing introns into elements construct unique addresses can be modified. We intron-containing ribosomes E. coli and perform of these using CRISPR/Cas9 multiplex automated engineering. Self-splicing post-transcription yields scarless RNA...
Abstract