A5011635552- RNA Research and Splicing
- RNA Interference and Gene Delivery
- RNA regulation and disease
- Signaling Pathways in Disease
- Nerve injury and regeneration
- MicroRNA in disease regulation
- RNA and protein synthesis mechanisms
- Cytokine Signaling Pathways and Interactions
- RNA modifications and cancer
- interferon and immune responses
University of South Carolina
2020-2022
Nucleolin is a multifunctional RNA Binding Protein (RBP) with diverse subcellular localizations, including the nucleolus in all eukaryotic cells, plasma membrane tumor and axon neurons. Here we show that glycine arginine rich (GAR) domain of nucleolin drives localization via protein-protein interactions kinesin light chain. In addition, GAR sequences mediate nucleolin. Both these modalities are addition to already reported involvement liquid-liquid phase separation nucleolus. transport axons...
Axonally synthesized proteins support nerve regeneration through retrograde signaling and local growth mechanisms. RNA binding (RBP) are needed for this other aspects of post-transcriptional regulation neuronal mRNAs, but only a limited number axonal RBPs known. We used targeted proteomics to profile in peripheral axons. detected 76 with reported activity axoplasm, levels several change axon injury regeneration. altered include KHSRP that decreases neurite outgrowth developing CNS neurons....
Abstract The KH-type splicing regulatory protein (KHSRP) is an RNA-binding linked to decay of mRNAs with AU-rich elements. KHSRP was previously shown destabilize Gap43 mRNA and decrease neurite growth in cultured embryonic neurons. Here, we have tested functions vivo. We find upregulation 1460 neocortex adult Khsrp −/− mice, which 527 bind high specificity. These targets are involved pathways for neuronal morphology, axon guidance, neurotransmission long-term memory. mice show increased...
ABSTRACT Proteins generated by localized mRNA translation in axons support nerve regeneration through retrograde injury signaling and axon growth mechanisms. RNA binding proteins (RBP) are needed for this other aspects of post-transcriptional control mRNAs, but only a limited number axonal RBPs have been reported. We used targeted mass spectrometry approach to profile the naïve, injured regenerating PNS axons. detected 76 that reported activity, with levels several these changing...
ABSTRACT The KH-type splicing regulatory protein (KHSRP) is an RNA-binding linked to decay of AU-rich element containing mRNAs. We have previously shown that KHSRP destabilizes the mRNA encoding growth-associated GAP-43 and decreases neurite growth in cultured embryonic neurons. In contrast, loss stabilizes Gap43 increases growth. Here, we tested functions neural vivo . find upregulation 1460 mRNAs neocortex adult Khsrp −/− mice, which 527 bind with high specificity. These targets are...