A5038549211- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Galectins and Cancer Biology
- Protein purification and stability
- Microfluidic and Bio-sensing Technologies
- CAR-T cell therapy research
- Angiogenesis and VEGF in Cancer
- Nanoparticle-Based Drug Delivery
- Phytochemistry and biological activity of medicinal plants
- Radiopharmaceutical Chemistry and Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Metabolomics and Mass Spectrometry Studies
- Transgenic Plants and Applications
- COVID-19 Clinical Research Studies
- Nigella sativa pharmacological applications
- RNA Interference and Gene Delivery
- Hepatitis Viruses Studies and Epidemiology
- Toxin Mechanisms and Immunotoxins
- Stress Responses and Cortisol
- Pharmacological Effects of Natural Compounds
- Immune Cell Function and Interaction
- RNA modifications and cancer
- Microfluidic and Capillary Electrophoresis Applications
- Bacteriophages and microbial interactions
- Reproductive System and Pregnancy
North Khorasan University of Medical Sciences
2016-2025
Pasteur Institute of Iran
2013-2017
Biotechnology Research Center
2015-2017
Islamic Azad University Tonekabon
2015
Nanobodies show attractive characteristics for tumor targeting in cancer diagnosis and therapy. A radiolabeled nanobody binding the prostate-specific membrane antigen (PSMA) could offer a noninvasive strategy to select prostate patients eligible PSMA-targeted therapies. We here describe generation, production vivo evaluation of anti-PSMA nanobodies. were derived from heavy-chain-only antibodies, raised immunized dromedaries. Binding evaluated through ELISA flow cytometry. Selected nanobodies...
Abstract: Immunotherapy is becoming an alternative method for gastrointestinal cancers, such as colorectal, gastric, and liver cancers. This field of research focuses on utilizing the immune system to recognize eliminate cancer cells. One important checkpoint inhibitors, which enable T cells attack tumor by releasing system's brakes. Chimeric antigen receptor (CAR) T-cell therapy another approach that modifies a patient's express receptors specific tumor-associated antigens. Some vaccines...
Alpha-synuclein (SNCA) as the presynaptic protein is expressed in different tissues and prevents insulin-resistance (IR) through increasing glucose-uptake by adipocytes muscles. However, effect of insulin metabolism on SNCA expression has scarcely elucidated. In present study we assessed probable resistance muscle C2C12 cells also skeletal type 2 diabetic mice. Sixteen male C57BL/6 mice were divided into two experimental groups, including control with IR (induced high-fat diet + low-dose...
Placental growth factor (PlGF) is a member of the vascular endothelial (VEGF) family. Unlike VEGF, PlGF dispensable for normal cell development as well playing various roles in pathological angiogenesis which occurs tissue ischemia, inflammation, and malignancy. The PlGF-1 has been considered potential candidate diagnosis targeting angiogenesis. Camelidae serum contains an important fraction functional antibodies, called heavy-chain antibodies (HcAbs) that are naturally devoid light chains....
Quantum dots (QDs) have widespread application in many fields such as medicine and electronics. The need for understanding the potentially harmful side effects of these materials becomes clear. In this study, toxicity cadmium telluride quantum (CdTe-QDs) bulk Cd2+ has been investigated compared by applying metabolomics methods. datasets were 1H-NMR data from mice plasma which had taken four groups different time intervals. Then, analyzed chemometrics methods metabolites found Human...
Angiogenesis which occurs mandatory in solid tumors, is a critical step malignancy progression. Vascular endothelial growth factor (VEGF) mainly responsible for angiogenesis process and facilitates the formation of new vessels. Distribution monoclonal antibodies against VEGF or receptor (VEGFR) into tumors limited because their huge dimensions. Moreover, many investigations have demonstrated usefulness immunotoxins to halt tumors.We designed, expressed evaluated cytotoxicity novel...
Hepatitis C virus (HCV) infection is a serious public health threat worldwide. Cellular immune responses, especially cytotoxic T-lymphocytes (CTLs), play critical role in response toward the HCV clearance. Since polytope vaccines have ability to stimulate cellular immunity, recombinant fusion protein was developed this study.The designed composed of hepatitis B surface antigen (HBsAg), as an immunocarrier, fused sequence. The containing five immunogenic epitopes induce specific CTL...
Angiogenesis, a neovascularization process, is the most important occurrence in developmental and pathological conditions. Key factors involve belonging to vascular endothelial growth factor family. Recently, placental (PlGF) has been considered medicine because of its importance solid tumor invasion metastasis. Therefore, PlGF targeting plays promising role hindrance progress. In this study, murine was cloned, expressed an Escherichia coli system, used for development polyclonal camel...
Antibody engineering is a dynamic field in antibody industry. Over 30% of novel monoclonal antibodies (mAbs) R&D and clinical trials are engineered forms. Affinity enhancement contributes to the development new binders that not only effective low dose cost but also improve some drawbacks production. After previous successful work on silico affinity maturation nanobody against placenta growth factor finding best nanobodies (Mut2:S31D Mut4:R45E), according bioinformatic parameters molecular...
Background: Molecular imaging is a highly effective method for diagnosing cancer and evaluating treatment. A molecular tracer often consists of two segments: targeting segment, which can be antibodies, antibody fragments, or VHH (nanobody), detection such as radioisotopes. The small size allows excellent tissue penetration fast clearance, resulting in minimal nonspecific background, makes them appealing use agents. 99m-technetium (99mTc), one the well-known radioisotopes, particularly useful...
Background: Circular RNAs (Circ RNA) are a large class of non-coding which particularlystable and mostly originate from gene exons in animals. circRNAs extremely abundantin the mammalian brains. They play role biological function development neurons.Nrf2 (nuclear factor erythroid 2–related 2) is transcription that regulates cellularantioxidants improves hippocampal synaptic plasticity, learning memory. The aim ofthis study evaluation Nrf2’s circRNA expression hippocampus rats duringoxidative...
Nanobody (Nb) or VHH is the smallest binding domain of camelid heavy chain antibody (HcAb). Light chains HcAb naturally removed and because some evolutionary changes, Nbs have unique properties rather than conventional antibodies. The size Nb about one-tenth (0.1) whole antibodies this improved problems four such as high yield expression in prokaryotic systems penetration to tissues. Some other characterizes like close homology human VH, stability extended range pH temperature, capability...
In the present study, we investigated association between complement system status at time of admission and clinical outcomes in COVID-19 patients. This single-center study was carried out with sixty-one adult patients who were hospitalized Imam Hassan Hospital North Khorasan University Medical Sciences (Bojnurd, Iran) less than three days passage since onset symptoms. Twenty-three healthy volunteers demographic features similar to patient group (matched by age gender) included as a control...
Placental growth factor (PlGF) is an angiogenic belonging to vascular endothelial family. This mainly expressed in the placenta and have important role blood supply embryonic tissues fetal. According accumulated data after 10th week of gestational age expression PlGF increased. The peak this seen 30th pregnancy. abnormal been some diseases such as preeclampsia, eclampsia, cancer, atherosclerotic lesions. Preeclampsia a pregnancy complication characterized by high pressure signs damage...