A5058338379- Pancreatic and Hepatic Oncology Research
- Silk-based biomaterials and applications
- Cancer Cells and Metastasis
- Spider Taxonomy and Behavior Studies
- Neurobiology and Insect Physiology Research
- Epigenetics and DNA Methylation
- Genomics and Phylogenetic Studies
- Biochemical and Structural Characterization
- Invertebrate Immune Response Mechanisms
- Antimicrobial Peptides and Activities
- Cellular Mechanics and Interactions
- Marine Ecology and Invasive Species
- Dental materials and restorations
- Freshwater macroinvertebrate diversity and ecology
- Insect behavior and control techniques
- Crustacean biology and ecology
- Building materials and conservation
- Aquatic Invertebrate Ecology and Behavior
- Developmental Biology and Gene Regulation
- Orthodontics and Dentofacial Orthopedics
- Facial Trauma and Fracture Management
- Marine and coastal plant biology
- Photopolymerization techniques and applications
- Aquatic life and conservation
- Marine and fisheries research
University of California, Riverside
2014-2021
Oregon Health & Science University
2018-2021
University of California, Berkeley
2009-2012
Integra (United States)
2009
Howard Hughes Medical Institute
2009
Reed College
2007
Cancer-associated fibroblast (CAF) heterogeneity is increasingly appreciated, but the origins and functions of distinct CAF subtypes remain poorly understood. The abundant transcriptionally diverse population in pancreatic ductal adenocarcinoma (PDAC) thought to arise from a common cell origin, stellate cells (PSC), with diversification resulting cytokine growth factor gradients within tumor microenvironment. Here we analyzed differentiation function PSCs during progression vivo. Contrary...
Spider silk research has largely focused on spidroins, proteins that are the primary components of spider fibers. Although a number spidroins have been characterized, other types associated with synthesis virtually unknown. Previous analyses tissue-specific RNA-seq libraries identified 647 predicted genes were differentially expressed in glands Western black widow, Latrodectus hesperus. Only ∼5% these silk-gland specific transcripts (SSTs) encode spidroins; although remaining presumably...
Spiders use a myriad of silk types for daily survival, and each type has unique suite task-specific mechanical properties. Of all spider types, pyriform is distinct because it combination dry protein fiber wet glue. Pyriform fibers are coated with cement extruded into "attachment discs" that adhere silks to other substrates. The properties linked the primary higher-level structures proteins (spidroins). Spidroins often enormous molecules (>250 kDa) have lengthy repetitive region flanked by...
Spider silks are spectacular examples of phenotypic diversity arising from adaptive molecular evolution. An individual spider can produce an array specialized silks, with the majority constituent silk proteins encoded by members spidroin gene family. Spidroins dominated tandem repeats flanked short, non-repetitive N- and C-terminal coding regions. The remarkable mechanical properties have been largely attributed to repeat sequences. However, evolutionary processes acting on terminal...
The great diversity of arthropod body plans, together with our detailed understanding fruit fly development, makes arthropods a premier taxon for examining the evolutionary diversification developmental patterns and hence extant life. Crustaceans, in particular, show remarkable range morphologies provide useful outgroup to insects. amphipod crustacean Parhyale hawaiensis is becoming established as model organism studies within arthropods. In addition its phylogenetically strategic position,...
Spider silk synthesis is an emerging model for the evolution of tissue-specific gene expression and role duplication in functional novelty, but its potential has not been fully realized. Accordingly, we quantified transcript (mRNA) abundance seven gland types three non-silk tissues cobweb-weaving spider species. Evolutionary analyses based on levels thousands homologous transcripts phylogenetic reconstruction 605 families demonstrated conservation each type among Despite serial homology all...
The great diversity of arthropod body plans, together with our detailed understanding fruit fly development, makes arthropods a premier taxon for examining the evolutionary diversification developmental patterns and hence extant life. Crustaceans, in particular, show remarkable range morphologies provide useful outgroup to insects. amphipod crustacean Parhyale hawaiensis is becoming established as model organism studies within arthropods. This protocol describes situ hybridization...
The great diversity of arthropod body plans, together with our detailed understanding fruit fly development, makes arthropods a premier taxon for examining the evolutionary diversification developmental patterns and hence extant life. Crustaceans, in particular, show remarkable range morphologies provide useful outgroup to insects. amphipod crustacean Parhyale hawaiensis is becoming established as model organism studies within arthropods. This protocol describes dissection fixation P....
Spiders (order Araneae) rely on their silks for essential tasks, such as dispersal, prey capture, and reproduction. Spider are largely composed of spidroins, members a protein family that synthesized in silk glands. As needed, stored glands is extruded through spigots the spinnerets. Nearly all studies spider have been conducted females; thus, little known about male biology. To shed light use by males, we compared gene expression profiles mature males to those females from three cob-web...
Spiders swath their eggs with silk to protect developing embryos and hatchlings. Egg case silks, like other fibrous spider are primarily composed of proteins called spidroins (spidroin = spider-fibroin). Silks, thus spidroins, important throughout the lives spiders, yet evolution spidroin genes has been relatively understudied. Spidroin notoriously difficult sequence because they typically very long (≥ 10 kb coding sequence) highly repetitive. Here, we investigate through long-read...
Abstract Filler particle functionalization with thiourethane oligomers has been shown to increase fracture toughness and decrease polymerization stress in dental composites, though the mechanism is poorly understood. The aim of this study was systematically characterize effect type filler surface on physicochemical properties experimental resin composites containing fillers different size volume fraction. Barium glass (1, 3 10 µm) were functionalized 2 wt% thiourethane-silane (TU-Sil)...
Abstract The cell movements of gastrulation were analyzed in embryos the spider Zygiella x‐notata , using time‐lapse video, tracing, and improved histology. Cells are internalized near center germ disc three distinct phases. First, cumulus mesenchyme cells ingress migrate as a group beneath superficial layer. Second, mass internalization through blastopore yields diffusely organized deep Third, accumulate at to form caudal bud. floor is internalized, bud moves over nascent dorsal field lobe....
INTRODUCTION The great diversity of arthropod body plans, together with our detailed understanding fruit fly development, makes arthropods a premier taxon for examining the evolutionary diversification developmental patterns and hence extant life. Crustaceans, in particular, show remarkable range morphologies provide useful outgroup to insects. amphipod crustacean Parhyale hawaiensis is becoming established as model organism studies within arthropods. This protocol describes injection P....
Most spiders spin multiple types of silk, including silks for reproduction, prey capture, and draglines. Spiders are a megadiverse group the majority spider remain uncharacterized. For example, nothing is known about silk molecules Tengella perfuga, that spins sheet webs lined with cribellar silk. Cribellar type adhesive capture thread composed numerous fibrils originate from specialized plate-like spinning organ called cribellum. The predominant components spidroins, members protein family...
Abstract Orb‐web weaving spiders produce a variety of task‐specific silks from specialized silk glands. The genetics underlying the synthesis specific types are largely unknown, and transcriptome analysis could be powerful approach for identifying candidate genes. However, de novo assembly expression profiling glands with RNA‐sequencing (RNAseq) problematic because few known gene transcripts proteins extremely long highly repetitive. To identify genes tubuliform (egg case) by orb‐weaver...
INTRODUCTION The great diversity of arthropod body plans, together with our detailed understanding fruit fly development, makes arthropods a premier taxon for examining the evolutionary diversification developmental patterns and hence extant life. Crustaceans, in particular, show remarkable range morphologies provide useful outgroup to insects. amphipod crustacean Parhyale hawaiensis is becoming established as model organism studies within arthropods. This protocol provides simplified...
Molecular studies of the secretory glands involved in spider silk production have revealed candidate genes for synthesis and a complicated history gene evolution. However, differential expression profiles multiple gland types within an individual orb-web weaving are lacking. Each these produces functionally distinct type. Comparison among would provide insight into that define generally from non-silk tissues, each other. Here, we perform 3' tag digital profiling seven silver garden orb...
The silk glands of the Western black widow Latrodectus hesperus Chamberlin & Ivie, 1935 are morphologically and functionally distinct. Studies spider often show only high magnification images sections or drawings glands, making differentiation dissected difficult. We dissect all gland types from L. females their gross morphology with light microscopy. Our micrographs portray distinct morphologies relative sizes each type, consistent prior descriptions apparatus spiders. Notably, we verify...
To survive in terrestrial and aquatic environments, spiders often rely heavily on their silk. The vast majority of silks that have been studied are from orb-web or cob-web weaving species, leaving the water-associated largely undescribed. We characterize transcripts, proteins, silk fibres semi-aquatic spider Dolomedes triton. From gland RNAseq libraries, we report 18 transcripts representing four categories known protein types: aciniform, ampullate, pyriform, tubuliform. Proteomic structural...
Abstract Cancer-associated fibroblast (CAF) heterogeneity is increasingly appreciated, but the origins and functions of distinct CAF subtypes remain poorly understood. The abundant transcriptionally diverse population in pancreatic ductal adenocarcinoma (PDAC) thought to arise from a common cell origin, stellate cells (PSCs), with diversification resulting cytokine growth factor gradients within tumor microenvironment. Here we analyzed differentiation function PSCs during progression vivo ....
<div>Abstract<p>Cancer-associated fibroblast (CAF) heterogeneity is increasingly appreciated, but the origins and functions of distinct CAF subtypes remain poorly understood. The abundant transcriptionally diverse population in pancreatic ductal adenocarcinoma (PDAC) thought to arise from a common cell origin, stellate cells (PSC), with diversification resulting cytokine growth factor gradients within tumor microenvironment. Here we analyzed differentiation function PSCs during...
<p>Figures S1-S3</p>
<p>Table S2</p>