A5084935468- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- Microtubule and mitosis dynamics
- Cellular Mechanics and Interactions
- Cellular transport and secretion
- Fungal Biology and Applications
- Microbial Metabolic Engineering and Bioproduction
- Antibiotic Use and Resistance
- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- Fungal and yeast genetics research
- Peptidase Inhibition and Analysis
- Cancer therapeutics and mechanisms
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
Drexel University
2023-2024
Centre for DNA Fingerprinting and Diagnostics
2018-2022
Manipal Academy of Higher Education
2018-2019
Long-range membrane traffic is guided by microtubule-associated proteins and posttranslational modifications, which collectively comprise a code. The regulatory principles of this code how it orchestrates the motility kinesin dynein motors are largely unknown. Septins large family GTP-binding proteins, assemble into complexes that associate with microtubules. Using single-molecule in vitro assays, we tested SEPT2/6/7, SEPT2/6/7/9, SEPT5/7/11 affect motilities constitutively active kinesins...
Known azole antifungal resistance mechanisms include mitochondrial dysfunction and overexpression of the sterol biosynthetic target enzyme multidrug efflux pumps. Here, we identify, through a genetic screen, vacuolar membrane-resident phosphatidylinositol 3-phosphate 5-kinase (CgFab1) to be novel determinant tolerance. We demonstrate for first time that fluconazole promotes actin cytoskeleton reorganization in emerging, inherently less azole-susceptible fungal pathogen Candida glabrata, or...
Considering the relevance of drug transporters belonging to ABC and MFS superfamilies in pathogenic Candida species, there has always been a need have an overexpression system where these membrane proteins for functional analysis could be expressed homologous background. We address this unmet by constructing highly drug-susceptible glabrata strain deleted seven dominant genes such as CgSNQ2, CgAUS1, CgCDR1, CgPDH1, CgYCF1, CgYBT1 CgYOR1 introduced GOF mutation transcription factor (TF)...
Increasing resistance of the human opportunistic fungal pathogen Candida glabrata toward echinocandin antifungals, which target cell wall, is a matter grave clinical concern. Echinocandin in C. has primarily been associated with mutations β-glucan synthase-encoding genes FKS1 ( CgFKS1 ) and CgFKS2 . This notwithstanding, role phosphoinositide signaling antifungal just beginning to be deciphered.
The microtubule cytoskeleton consists of subsets with distinct compositions microtubule-associated proteins, which instruct the position and traffic subcellular organelles. In endocytic pathway, these cues are poorly understood. Here, we report that in MDCK cells, endosomes multivesicular body (MVB) late endosome (LE) markers localize preferentially to microtubules coated septin GTPases. Compared early endosomes, CD63-containing MVBs/LEs largely immotile on septin-coated microtubules. vitro...