A5056782550- Mitochondrial Function and Pathology
- Muscle Physiology and Disorders
- Autophagy in Disease and Therapy
- Adipose Tissue and Metabolism
- RNA Research and Splicing
- Cancer, Hypoxia, and Metabolism
- Molecular Biology Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Histone Deacetylase Inhibitors Research
- RNA modifications and cancer
- Neurogenesis and neuroplasticity mechanisms
- ATP Synthase and ATPases Research
- Cancer-related molecular mechanisms research
- Heat shock proteins research
University of Ottawa
2022-2024
Carleton University
2020-2023
Quiescence regulation is essential for adult stem cell maintenance and sustained regeneration. Our studies uncovered that physiological changes in mitochondrial shape regulate the quiescent state of muscle cells (MuSCs). We show MuSC mitochondria rapidly fragment upon an activation stimulus, via systemic HGF/mTOR, to drive exit from deep quiescence. Deletion fusion protein OPA1 fragmentation transitions MuSCs into G-alert quiescence, causing premature depletion a stimulus. loss activates...
Abstract Impaired mitochondrial function is a hallmark of aging and major contributor to neurodegenerative diseases. We have shown that disrupted dynamics typically found in alters the fate neural stem cells (NSCs) leading impairments learning memory. At present, little known regarding mechanisms by which progenitor survive adapt dysfunction. Using Opa1‐inducible knockout as model neurodegeneration, we identify decline neurogenesis due impaired cell activation proliferation, can be rescued...
Since changes in mitochondrial morphology regulate key functions of stem cells, it is important to assess their structure under physiological and pathophysiological conditions. Here, we present techniques optimized rare adult muscle cells (MuSCs). For evaluating length volume within a compact cytoplasmic area MuSCs on intact myofibers, describe steps for staining, imaging, quantification. ultrastructure small cell numbers, agarose embedding quantification by TEM. complete details generation...
Abstract Freeze tolerance is an adaptive strategy that wood frogs ( Rana sylvatica ) use to survive the subzero temperatures of winter. It characterized by a variety metabolic and physiological changes facilitate successful freezing anoxia. As both mRNA regulation posttranslation protein modification have been implicated in freeze tolerance, we hypothesized posttranslational RNA also involved coordinating freeze‐thaw cycles rate depression. such, investigated most abundant modification,...
The African clawed frog (Xenopus laevis) withstands prolonged periods of extreme whole-body dehydration that lead to impaired blood flow, global hypoxia, and ischemic stress. During dehydration, these frogs shift from oxidative metabolism a reliance on anaerobic glycolysis. In this study, we purified the central glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) electrophoretic homogeneity investigated structural, kinetic, subcellular localization, post-translational...