Marco Mattioli
A5028496079- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Long-Term Effects of COVID-19
- COVID-19 Impact on Reproduction
- Neuroinflammation and Neurodegeneration Mechanisms
- Multiple Sclerosis Research Studies
- Immune Response and Inflammation
- Metabolism and Genetic Disorders
- Reproductive System and Pregnancy
- Pregnancy and Medication Impact
- Exercise and Physiological Responses
- Adipokines, Inflammation, and Metabolic Diseases
- Diabetes and associated disorders
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- interferon and immune responses
- Schizophrenia research and treatment
- Intensive Care Unit Cognitive Disorders
- Immune cells in cancer
- COVID-19 epidemiological studies
- Immune Cell Function and Interaction
- Mental Health Treatment and Access
- Hereditary Neurological Disorders
- HIV/AIDS drug development and treatment
- Tendon Structure and Treatment
- Thermoregulation and physiological responses
University of Modena and Reggio Emilia
2019-2023
Sapienza University of Rome
2016
Abstract The immune system of patients infected by SARS-CoV-2 is severely impaired. Detailed investigation T cells and cytokine production in affected COVID-19 pneumonia are urgently required. Here we show that, compared with healthy controls, patients’ cell compartment displays several alterations involving naïve, central memory, effector memory terminally differentiated cells, as well regulatory PD1 + CD57 exhausted cells. Significant exist also lineage-specifying transcription factors...
In patients infected by SARS-CoV-2 who experience an exaggerated inflammation leading to pneumonia, monocytes likely play a major role but have received poor attention. Thus, we analyzed peripheral blood from with COVID-19 pneumonia and found that these cells show signs of altered bioenergetics mitochondrial dysfunction, had reduced basal maximal respiration, spare respiratory capacity, decreased proton leak. Basal extracellular acidification rate was also diminished, suggesting capability...
Studies on the interactions between SARS-CoV-2 and humoral immunity are fundamental to elaborate effective therapies including vaccines. We used polychromatic flow cytometry, coupled with unsupervised data analysis principal component (PCA), interrogate B cells in untreated patients COVID-19 pneumonia. displayed normal plasma levels of main immunoglobulin classes, antibodies against common antigens or present However, we found a decreased number total naïve cells, along percentages numbers...
Abstract We have deeply investigated T cell compartment, plasma cytokines and cells producing in patients affected by Covid-19. At admission, were lymphopenic; all of them SARS-CoV-2 was detected a nasopharyngeal swab specimen real-time RT-PCR, pneumonia subsequently confirmed X-rays.Detailed 18-parameter flow cytometry performed 21 13 controls. Coupling polychromatic with unsupervised data analysis, we found that show an increased amount CD4+ lymphocytes activated, exhausted, stem memory or...
Background and purpose Lower urinary tract symptoms ( LUTS ) including frequent urination, nocturia urge incontinence negatively impact quality of life. This project aimed at characterizing the prevalence severity in multiple sclerosis MS patients its association with demographic clinical features. Methods In all, 403 consecutive clinically stable answered International Consultation on Incontinence Questionnaire ICIQ Patient Perception Bladder Condition PPBC questionnaire. Demographic...
To better understand the mechanisms at basis of neutrophil functions during SARS-CoV-2, we studied patients with severe COVID-19 pneumonia. They had high blood proportion degranulated neutrophils and elevated plasma levels myeloperoxidase (MPO), elastase, MPO-DNA complexes, which are typical markers extracellular traps (NET). Their display dysfunctional mitochondria, defective oxidative burst, increased glycolysis, glycogen accumulation in cytoplasm, increase glycogenolysis....
Abstract Aging is a major risk factor for developing severe COVID-19, but few detailed data are available concerning immunological changes after infection in aged individuals. Here we describe main immune characteristics 31 patients with SARS-CoV-2 who were >70 years old, compared to 33 subjects <60 of age. Differences plasma levels 62 cytokines, landscape peripheral blood mononuclear cells, T cell repertoire, transcriptome central memory CD4 + specific antibodies reported along...
Abstract We report two fatal cases of acute liver failure secondary to herpes simplex virus 1 infection in COVID-19 patients, following tocilizumab and corticosteroid therapy. Screening for prompt recognition reactivation these undergoing immunomodulatory treatment, may have potentially relevant clinical consequences.
Abstract SARS-CoV-2 infection can affect all human beings, including pregnant women. Thus, understanding the immunological changes induced by virus during pregnancy is nowadays of pivotal importance. Here, using peripheral blood from 14 women with asymptomatic or mild infection, we investigate cell proliferation and cytokine production, measure plasma levels 62 cytokines, perform a 38-parameter mass cytometry analysis. Our results show an increase in low density neutrophils but no...
Although it is now widely accepted that host inflammatory response contributes to COVID-19 immunopathogenesis, the pathways and mechanisms driving disease severity clinical outcome remain poorly understood. In effort identify key soluble mediators characterize life-threatening COVID-19, we quantified 62 cytokines, chemokines other factors involved in inflammation immunity plasma samples, collected at hospital admission, from 80 hospitalized patients with severe who were stratified on basis...
The formation of a robust long-term antigen (Ag)-specific memory, both humoral and cell-mediated, is created following severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection or vaccination. Here, by using polychromatic flow cytometry complex data analyses, we deeply investigated the magnitude, phenotype, functionality SARS-CoV-2-specific immune memory in two groups healthy subjects after heterologous vaccination compared to group who recovered from SARS-CoV-2 infection. We...
The pandemic caused by severe acute respiratory syndrome coronavirus 2 heavily involves all those working in a laboratory. Samples from known infected patients or donors who are considered healthy can arrive, and colleague might be asymptomatic but able to transmit the virus. Working clinical laboratory is posing several safety challenges. Few years ago, International Society for Advancement of Cytometry published guidelines safely analyze sort human samples that were revised these days. We...
Specific T cells are crucial to control SARS-CoV-2 infection, avoid reinfection and confer protection after vaccination. We have studied patients with severe or moderate COVID-19 pneumonia, compared who recovered from a infection that had occurred about 4 months before the analyses. In all these subjects, we assessed polyfunctionality of virus-specific CD4+ CD8+ by quantifying cytokine production in vitro stimulation different peptide pools covering proteins (M, N S). particular, quantified...
Introduction A growing number of evidences suggest that the combination hyperinflammation, dysregulated T and B cell response cytokine storm play a major role in immunopathogenesis severe COVID-19. IL-6 is one main pro-inflammatory cytokines its levels are increased during SARS-CoV-2 infection. Several observational randomized studies demonstrated tocilizumab, an IL-6R blocker, improves survival critically ill patients both infectious disease intensive care units. However, despite...
Whole-body cryotherapy (WBC) consists of short exposure (up to 2-3 min) dry air at cryogenic temperatures -190 °C) and has recently been applied for muscle recovery after injury reduce the inflammation process. We aimed determine impact on immunological, hormonal, metabolic responses in non-professional soccer players (NPSPs). Nine male NPSPs (age: 20 ± 2 years) who trained regularly over 5 consecutive days, immediately before each training session, were subjected WBC treatment (WBC-t)....
The aim of this randomized clinical trial (RCT) was to compare immunological changes in virally suppressed people living with HIV (PLWH) switching from a three-drug regimen (3DR) two-drug (2DR).An open-label, prospective RCT enrolling PLWH receiving 3DR who switched bictegravir/emtricitabine/tenofovir alafenamide (B/F/TAF) or dolutegravir/lamivudine (DTG/3TC) performed. Blood taken at baseline and months 6 12. primary outcome the change CD4+ CD8+ T-cell counts CD4/CD8 ratio over time points....