A5071411702- Antibiotic Resistance in Bacteria
- Listeria monocytogenes in Food Safety
- Salmonella and Campylobacter epidemiology
- Bacteriophages and microbial interactions
- Identification and Quantification in Food
- Microbial Inactivation Methods
- Enterobacteriaceae and Cronobacter Research
- Bacterial biofilms and quorum sensing
- Microbial infections and disease research
- Molecular Biology Techniques and Applications
- Genomics and Phylogenetic Studies
- Viral gastroenteritis research and epidemiology
- Bacterial Identification and Susceptibility Testing
- Mycobacterium research and diagnosis
- Pharmaceutical and Antibiotic Environmental Impacts
- Hepatitis B Virus Studies
- Milk Quality and Mastitis in Dairy Cows
- Corneal surgery and disorders
- Antimicrobial Resistance in Staphylococcus
- Advanced Chemical Sensor Technologies
- Tuberculosis Research and Epidemiology
- Antimicrobial Peptides and Activities
- Yersinia bacterium, plague, ectoparasites research
- Aquaculture disease management and microbiota
- Antimicrobial agents and applications
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale
2017-2024
University of Cagliari
2020
The Klebsiella pneumoniae species complex (KpSC) is a leading cause of multidrug-resistant human infections. To better understand the potential contribution food as vehicle KpSC, we conducted multicentric study to define an optimal culture method for its recovery from matrices and characterize isolates phenotypically genotypically. Chicken meat (n = 160) salad 145) samples were collected in five European countries screened presence KpSC using culture-based zur-khe intergenic region (ZKIR)...
Food packaging is not only a simple protective barrier, but real “active” component, which expected to preserve food quality, safety and shelf-life. Therefore, the materials used for production should show peculiar features properties. Specifically, antimicrobial has recently gained great attention with respect both social economic impacts. In this paper, results obtained by using polymer material functionalized small synthetic peptide as are reported. The surface of Polyethylene...
Wastewater treatment plants are hotspots for the release of antimicrobial resistant pathogenic bacteria into aquatic ecosystems, significantly contributing to cycle resistance. Special attention should be paid ESKAPE bacteria, which have been identified as high-priority targets control measures. Among them, Klebsiella pneumoniae is particularly noteworthy. In this study, we collected wastewater samples from inlet, sedimentation tank, and effluent water a plant in June, July, October,...
The contamination of ready to eat foods (RTE) products due Listeria monocytogenes could compromise the safety becoming a great risk for consumers. high presence L. in RTE has been described worldwide, but few data are available about these from African countries. aims this study were report Zambian products, providing genomic characterization and on similarity with circulating strains using whole genome sequencing (WGS). A total 304 produced by different manufacturers, purchased at retail,...
Carbapenemase-producing
Despite Klebsiella pneumoniae being widely recognized as a nosocomial pathogen, there is critical lack in defining its reservoirs and sources of infections. Most studies on risk factors have focused multidrug-resistant (MDR) isolates clinically-oriented questions. Over two-year period, we sampled 131 wild animals including mammal bird species from three regions Central Italy. All typical colonies isolated the analytical portions were confirmed by real-time PCR identified MALDI-TOF mass...
Listeria monocytogenes (L.m.) is an agent of serious foodborne illness. It a major concern for the food industry, since microorganism, growing in biofilms protected against cleaning and disinfection difficult to eradicate. Aim this study was develop protocol assess ability two flexible packaging materials, named HGP40 GND35, submitted corona discharge treatment, limit production L.m. biofilm at 12±1°C. Two strains were selected study: L.m ATCC 7644 L. m. EURL 12MOB098LM isolated from dairy...
From May 2015 to March 2016, a severe outbreak due Listeria monocytogenes ST7 strain occurred in Central Italy and caused 24 confirmed clinical cases. The epidemic was deeply investigated using whole-genome sequencing (WGS) analysis. In the interested area, foodborne investigation identified meat food-producing plant contaminated by strain, carried pork-ready-to-eat products. same region, 2018, reemerged causing one listeriosis case 10-month-old child. aim of this study investigate phylogeny...
ABSTRACT We report the whole-genome sequences of two Listeria monocytogenes strains responsible for a severe invasive listeriosis outbreak in central Italy that occurred 2015 and 2016. These differ by single band their pulsed-field gel electrophoresis (PFGE) profiles.
Human salmonellosis incidence is increasing in the European Union (EU). Salmonellaenterica subsp. enterica serovar Enteriditis, Typhimurium (including its monophasic variant) and Infantis represent targets control programs due to their frequent association with human cases. This study aimed detect most prevalent serotypes circulating Abruzzo Molise Regions between 2015 2020 framework of Italian National Control Program for Salmonellosis Poultry (PNCS)]. A total 332 flocks were sampled by...
In Europe, very few studies are available regarding the diversity of
The rapid emergence of carbapenem-resistant Klebsiella pneumoniae ( Kp ) strains in diverse environmental niches, even outside the clinical setting, poses a challenge for detection and real-time monitoring novel antimicrobial resistance trends using molecular whole genome sequencing-based methods. aim our study was to understand cryptic determinants responsible phenotypic carbapenem observed circulating Italy by combined approach involving sequencing (WGS) genome-wide association (GWAS). In...
This report describes the draft genomes of two Klebsiella pneumoniae strains that were isolated from wild boars collected during epidemiological surveillance and monitoring fauna in Abruzzo Molise regions. The belonged to sequence type 23 (ST23) ST35, which are frequently reported clinical cases.
Here, we report the genome sequence of Listeriamonocytogenes serovar 1/2a strain IZSAM_Lm_15_17439_A144, isolated in Italy from a patient during Listeria monocytogenes outbreak 2008. This showed 98.9% identity to Canada same year.
Abstract Interpreting phenotypes of bla SHV alleles in Klebsiella pneumoniae genomes is complex. While all strains are expected to carry a chromosomal copy conferring resistance ampicillin, they may also mutations or additional plasmid-borne that have extended-spectrum β-lactamase (ESBL) activity and/or inhibitor (BLI) activity. In addition, the role individual mutations/amino acid changes not completely documented understood. This has led confusion literature and antimicrobial (AMR) gene...
Serratia rubidaea has emerged in recent years as an opportunistic nosocomial pathogen. Here, we present the draft genome sequence of isolate derived from industrial meat food product purchased a large-scale retail store that revealed fluoroquinolone, β-lactam, and aminoglycoside resistance genes two different host-unspecific prophages.
Abstract Background Listeriosis is a life-threatening disease in human as well animals, cows and sheep are the most sensible to disease. Correlation feed documented (silage). Some clonal complexes (CCs) more virulent than others frequently involved animal listeriosis. Whole genome sequencing (WGS) silico CCs identification core multilocus sequence typing (cgMLST) determination best way characterize isolates confirm connection between clinical strains source of contamination. This work aimed...
Abstract Background Klebsiella pneumoniae (Kp) is considered one of the major causes human hospital infections. Moreover, Kp often present in several food products and it could be a potential health hazard for consumers through increase antimicrobial resistance (AMR) to main routinely used antibiotics classes. The aim this study was evaluate AMR profile strains isolated from vegetable products. Methods whole genome sequences 10 strains, previously carrots (n. 4), salads 3), tomatoes 2)...
Abstract Background Klebsiella pneumoniae (Kp) is an opportunistic pathogen now acknowledged as urgent threat to human health because it multi-drug resistant bacteria. The role of food reservoir or carrier Kp under investigation, at the same time optimization method detect in ongoing. study was aimed carrots using conventional and screen Real-time PCR performances, targeting intergenic sequence between zur khe, based on INRA Pasteur Institute Methods A total 60 samples were tested. (25 g)...
Abstract Background Klebsiella pneumoniae (Kp) is a Gram-negative hospital-acquired pathogen. Kp also poses potential food safety hazard, since significant increase of multidrug-resistant (MDR) was observed in foods. The purpose this study to characterize MDR strains order evaluate the presence beta-lactams, quinolones and aminoglycosides resistance genes. Methods A total 13 strains, isolated from chicken (n.8), raw milk (n.2), waste water human faeces (n.1), identified as by microdilution...
The aim of this study was to evaluate the performance "Micro Biological Survey - MBS Test" in enumeration bacterial load cow raw milk. test is based on a colorimetric method recently developed and patented by "Roma Tre" University, Italy. evaluation carried out comparison with plate count at 30°C (gold standard) flow cytometry. Thirteen independent set experiments were performed analyzing total 104 samples milk selected methods. Results obtained using are comparable those (CFU/mL) cytometry...