A5051749495- Listeria monocytogenes in Food Safety
- Salmonella and Campylobacter epidemiology
- Microbial Inactivation Methods
- Food Safety and Hygiene
- Essential Oils and Antimicrobial Activity
- Molecular Biology Techniques and Applications
- Myxozoan Parasites in Aquatic Species
- Infectious Diseases and Mycology
- Identification and Quantification in Food
- Actinomycetales infections and treatment
- Spectroscopy Techniques in Biomedical and Chemical Research
- Medical Device Sterilization and Disinfection
- Viral Infections and Vectors
- Spectroscopy and Chemometric Analyses
- Bacteriophages and microbial interactions
- Plant Disease Resistance and Genetics
- Yersinia bacterium, plague, ectoparasites research
- Viral gastroenteritis research and epidemiology
- Antimicrobial agents and applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Advanced Chemical Sensor Technologies
- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Biosensors and Analytical Detection
- Protein Hydrolysis and Bioactive Peptides
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale
2013-2025
ABSTRACT Listeria monocytogenes presents a significant concern for the food industry due to its ability persist in processing environment. One of factors contributing persistence is decreased sensitivity disinfectants. Our objective was assess diversity L. disinfectants by testing response 1,671 L . isolates quaternary ammonium compounds (QACs) and 414 peracetic acid (PAA) using broth microdilution growth curve analysis assays, respectively, categorize into sensitive tolerant. A high...
ABSTRACT Jaagsiekte sheep retrovirus (JSRV) is the causative agent of ovine pulmonary adenocarcinoma (OPA). The expression JSRV envelope (Env) alone sufficient to transform a variety cell lines in vitro and induce lung cancer immunodeficient mice. In order determine role Env OPA tumorigenesis sheep, we derived replication-defective virus (JS-RD) which expresses env under control its own long terminal repeat (LTR). JS-RD was produced by transiently transfecting 293T cells with two plasmid...
Purpose. From May 2015 to March 2016, an outbreak due Listeria monocytogenes serotype 1/2a and clinical pulsotype never previously isolated in Europe occurred central Italy, involving 24 confirmed cases. The article provides a description of the investigation carried out by multidisciplinary network. Methodology. Epidemiological microbiological surveillance was conducted confirm detect food vehicle infection. origin destination implicated its ingredients were investigated tracing-back...
A total of 66 Listeria monocytogenes (Lm) isolated from 2013 to 2018 in a small-scale meat processing plant and dairy facility Central Italy were studied. Whole Genome Sequencing bioinformatics analysis used assess the genetic relationships between strains investigate persistence virulence abilities. The biofilm forming-ability was assessed vitro. Cluster grouped Lm into three main clusters: two them, both belonging CC9, persisted for years one (CC121) last year sampling. In facility, all...
Listeriosis, caused by Listeria monocytogenes , is a severe foodborne infection, particularly hazardous for pregnant women and immunocompromised individuals. In Europe, L. was the fifth most reported zoonotic agent in 2022, with outbreaks frequently linked to ready‐to‐eat (RTE) foods. Addressing food contamination innovative antimicrobial strategies critical enhancing safety reducing public health risks. This study evaluated efficacy of two bacteriophage‐derived endolysins, LP101_021...
In 2012 a US multistate outbreak of listeriosis was linked to ricotta salata imported from Italy, made pasteurized sheep's milk. Sampling activities were conducted in Italy trace the source Listeria monocytogenes contamination. The cheese that caused produced plant Apulia processed semi-finished cheeses supplied by five plants Sardinia. During an 'emergency sampling', 179 (23·6%) out 758 end-products tested positive for L. monocytogenes, with concentrations <10 c.f.u./g 1·1 × 106 c.f.u./g....
Understanding the factors governing host species barriers to virus transmission has added significantly our appreciation of pathogenesis. Jaagsiekte sheep retrovirus (JSRV) is causative agent ovine pulmonary adenocarcinoma (OPA), a transmissible lung cancer that rarely been found in goats. In this study, order further clarify pathogenesis OPA, we investigated whether goats are resistant JSRV replication and carcinogenesis. We induces tumors with macroscopic histopathological features...
From January 2015 to March 2016, an outbreak of 23 human cases listeriosis in the Marche region and one case Umbria Italy was caused by Listeria monocytogenes strains showing a new pulsotype never described before Italy. A total 37 clinical isolated from patients exhibiting symptoms 1374 correlated were received Italian National Reference Laboratory for L. (It NRL Lm) Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise (IZSAM) investigation. real-time PCR assay purposely designed...
ABSTRACT Listeria monocytogenes is a significant concern for the food industry due to its ability persist in processing environment. Decreased susceptibility disinfectants one of factors that contribute persistence L. . The objective this study was explore diversity quaternary ammonium compounds (QACs) using 1,671 isolates. This used determine phenotype-genotype concordance and characterize genomes QAC sensitive tolerant isolates stress resistance, virulence plasmid replicon genes....
Listeria monocytogenes (L.m.) is an agent of serious foodborne illness. It a major concern for the food industry, since microorganism, growing in biofilms protected against cleaning and disinfection difficult to eradicate. Aim this study was develop protocol assess ability two flexible packaging materials, named HGP40 GND35, submitted corona discharge treatment, limit production L.m. biofilm at 12±1°C. Two strains were selected study: L.m ATCC 7644 L. m. EURL 12MOB098LM isolated from dairy...
From May 2015 to March 2016, a severe outbreak due Listeria monocytogenes ST7 strain occurred in Central Italy and caused 24 confirmed clinical cases. The epidemic was deeply investigated using whole-genome sequencing (WGS) analysis. In the interested area, foodborne investigation identified meat food-producing plant contaminated by strain, carried pork-ready-to-eat products. same region, 2018, reemerged causing one listeriosis case 10-month-old child. aim of this study investigate phylogeny...
ABSTRACT We report the whole-genome sequences of two Listeria monocytogenes strains responsible for a severe invasive listeriosis outbreak in central Italy that occurred 2015 and 2016. These differ by single band their pulsed-field gel electrophoresis (PFGE) profiles.
We report the whole-genome sequence of a Listeria monocytogenes strain isolated from child in central Italy. Interestingly, showed difference only 13 single-nucleotide polymorphisms (SNPs) responsible for severe listeriosis outbreak that occurred between January 2015 and March 2016 same region.
Bluetongue (BT) is non-contagious, vector-borne viral disease of domestic and wild ruminants, transmitted by midges (Culicoides spp.) caused virus (BTV). BTV the type species Orbivirus genus within Reoviridae family possesses a genome consisting 10 double-stranded RNA segments encoding 7 structural 4 nonstructural proteins. Viral Protein (VP7) major sera group-specific protein good antigen candidate for immunoenzymatic assays BT diagnosis. In our work, BTV-2 recombinant VP7 (BTV-2 recVP7),...
Yersinia enterocolitica causes foodborne disease in humans and infections are usually acquired from contaminated raw or undercooked pork. Pigs considered the primary reservoir of human pathogenic bio-serotypes. A total 376 tonsil tissue samples collected after evisceration cutting pig carcasses were tested for enterocolitica. Animals came an abattoir located Abruzzo region, Italy. was isolated 35 out (9.31%) samples. 47 strains isolated, prevalent bio-serotype 4÷O:3 (95.74%), followed by...
Listeria monocytogenes is one of the most important foodborne pathogens. In this report, we present complete and annotated genome L. sequence type 06 (ST06) serovar 4b strain IZSAM_Lm_hs2008, isolated from an adult immunocompetent patient who developed disease died.
Here, we report the genome sequence of Listeriamonocytogenes serovar 1/2a strain IZSAM_Lm_15_17439_A144, isolated in Italy from a patient during Listeria monocytogenes outbreak 2008. This showed 98.9% identity to Canada same year.
Abstract This project, titled ‘Listeria Control,’ aimed to advance expertise across Europe in applying predictive microbiology shelf‐life studies of Listeria monocytogenes ready‐to‐eat (RTE) products. By increasing the capacity two participating organisations microbiology, this initiative strengthens Europe's overall ability manage and mitigate risk L. RTE foods. The project's first experimental phase involved trials that examined growth under both constant dynamic temperature conditions....
Abstract Background In 2019, case fatality from Listeria monocytogenes (Lm) was higher than the previous years confirming Lm as a major public health problem. Food is main source of infection and cross-contamination food-processing environment (FPEs) most common route contamination. The aim this work to characterize FPEs strains collected National Reference Laboratory (NRL) for in order evaluate circulating clonal complexes (CCs) their environmental adaptation. Methods NRL database actually...
Abstract Background In literature, there are no standardized laboratory methods to detect formed biomass by colorimetric analysis. The purpose of this study was compare three staining and two different wavelengths for determination biofilm formation Listeria monocytogenes (Lm) strains. Methods Three strains Lm isolated from origin were tested using 96 well polistirene plates at 12 °C 30 °C, after incubation the wells subjected washing, detaching with crystal violet (CV) 0.2% 2% (Panreac EU)...
Abstract Background Studies checking antimicrobial resistance (AMR) of Listeria monocytogenes (Lm) are ongoing, coupled with disease surveillance. Lm resistant strains reported and increasing. The aim the work was to investigate AMR clinical collected from nine regions (mostly in Lombardy Marche regions) 2008 2020. Methods dataset consisted 233 human during period 2008-2020. All were tested using Sensititre™ Haemophilus Plate (Thermo Scientific, Milano, Italy). Results expressed according...