- Listeria monocytogenes in Food Safety
- Salmonella and Campylobacter epidemiology
- Food Safety and Hygiene
- Identification and Quantification in Food
- Probiotics and Fermented Foods
- Escherichia coli research studies
- Viral gastroenteritis research and epidemiology
- Spectroscopy and Chemometric Analyses
- Milk Quality and Mastitis in Dairy Cows
- Carbohydrate Chemistry and Synthesis
- T-cell and Retrovirus Studies
- Protein Hydrolysis and Bioactive Peptides
- Essential Oils and Antimicrobial Activity
- Enterobacteriaceae and Cronobacter Research
- Microbial Metabolism and Applications
- Animal Disease Management and Epidemiology
- Meat and Animal Product Quality
- Vibrio bacteria research studies
- Aquaculture disease management and microbiota
- Microbial Inactivation Methods
- Mycobacterium research and diagnosis
- Pesticide Residue Analysis and Safety
- Advanced Statistical Process Monitoring
- Infant Nutrition and Health
- Optics and Image Analysis
Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche
2009-2022
United States Nuclear Regulatory Commission
2006
University of Bologna
2000
The majority of proteins in cow’s milk are caseins, which occur four groups (α-s1, α-s2, β, and k) encoded by different genes (CSN1S1, CSN1S2, CSN2, CSN3, respectively). In this study, we focused on the β-casein allele variants A1 A2 due to their influence milk’s technological characteristics human health. Digestion variant leads formation β-casomorphin 7 (BCM-7), a bioactive peptide that has been suggested be possible cause various diseases associated with low digestibility. potential...
This study investigated two foodborne outbreaks of gastroenteritis that occurred 10 days apart among individuals who had meals at the restaurant a farm holiday resort. Mild gastrointestinal symptoms were reported and none patients needed hospitalization. Mean incubation times 45 33 h, overall attack rates 43.5 58.3%, respectively. Stool sample examination was negative for common enteric pathogens in both outbreaks. Specimens from 13 people involved second outbreak 3 staff examined...
Many studies highlighted potential associations of β-casein A1 with specific human diseases and a minor digestibility milk, due to the bioactive peptide β-casomorphin 7 (BCM-7) release during digestion. Conversely, ancestral A2 variant seems be favorable trait because it is not associated BMC-7 release. The aim this work was evaluate frequencies variants in offspring previously genotyped cows inseminated homozygous semen. frequency A2/A2 animals has almost doubled from 37 69%. These are...
Purpose. From May 2015 to March 2016, an outbreak due Listeria monocytogenes serotype 1/2a and clinical pulsotype never previously isolated in Europe occurred central Italy, involving 24 confirmed cases. The article provides a description of the investigation carried out by multidisciplinary network. Methodology. Epidemiological microbiological surveillance was conducted confirm detect food vehicle infection. origin destination implicated its ingredients were investigated tracing-back...
A total of 66 Listeria monocytogenes (Lm) isolated from 2013 to 2018 in a small-scale meat processing plant and dairy facility Central Italy were studied. Whole Genome Sequencing bioinformatics analysis used assess the genetic relationships between strains investigate persistence virulence abilities. The biofilm forming-ability was assessed vitro. Cluster grouped Lm into three main clusters: two them, both belonging CC9, persisted for years one (CC121) last year sampling. In facility, all...
Listeria monocytogenes (Lm) can persist in food processing environments (FPEs), surviving environmental stresses and disinfectants. We described an intensive monitoring plan performed Central Italy involving producing plants (FPPs) retail grocery stores (RSs). The aim of the study was to provide a snapshot Lm circulation different FPEs during severe listeriosis outbreak, using whole genome sequencing (WGS) investigate genetic diversity isolated, evaluating their virulence stress resistance...
Salmonellosis is the second most commonly reported gastrointestinal infection in humans after campylobacteriosis, and an important cause of foodborne outbreaks EU/EEA. The vast majority (72.4%) salmonellosis EU 2019 were caused by Salmonella Enteritidis, even if their total number due to this serovar decreased. In spring 2020, a outbreak S. Enteritidis occurred Marche region (Central Italy), involving 85 people. common exposure source was cheese, pecorino "primo sale", produced with raw...
The aim of the present study was to investigate occurrence Listeria monocytogenes in salami samples collected from production plants Marche Region, and assess end-product acceptability based on former Italian regulations European Commission (EC) Regulation No 2073/2005. Based limits specified regulations, percentage non-acceptable 34.3%, whereas EC N degrees 2073/2005, a lower (17.1%) seen. A similar trend seen also when only Ciauscolo were considered, with 45.2 27.4% samples, respectively....
Colibacillosis is the most frequent bacterial disease in avian species and antimicrobials are main weapon to reduce incidence mortality associated it. However, indiscriminate use of antibiotics may lead therapy failure economic losses for breeder. The aims this study were to, determine antibiotic resistance Escherichia coli isolates, evaluate correlation between E. isolation systems breeding included study, identify pathogenic E.coli (APEC) amongst strains isolated. A total 51 subjected...
Summary Bdellovibrio bacteriovorus is a predator micro‐organism towards other Gram‐negative bacteria. We tested B. to control Escherichia coli growth on chicken slices and canned beef. Moreover, we analysed bacteriovorus's lytic ability eight toxigenic or multidrug‐resistant E. strains. In slices, the induced highest prey reduction (4.3 log) respect at 6 h. beef, (2.1 showed all could pathogenic spoilage bacteria in those meat‐based foods that have shelf life <10 days. It integrate...
Infection remains a major problem for individuals who undergo solid organ transplantation and liver transplant recipients are particularly susceptible to infectious complications with high morbidity mortality rate. The risk of these infections is determined by previous or future environmental exposures as well the patient's immune status. We report here results an open prospective study involving 49 consecutive transplantations, undertaken evaluate efficacy ceftriaxone in prevention early...
In the last century, exponential increase of industrial food production led to disappearance "Italian traditional niche products". However, national regulations allowed preservation several these products, including burrata cheese. Twenty-one samples from three different batches "Burrata di Andria" Protected Geographical Indication (PGI) were purchased dairy factories PGI consortium. Moisture value Burrata cheese was significantly higher than that before release. Moreover, a lower NaCl...
The public health risk posed by Listeria monocytogenes in ready-to-eat (RTE) foods depends on the effectiveness of its control at every stage production process and strain involved. Analytical methods currently use are limited to identification/quantification L. species level, without distinguishing virulent from hypovirulent strains. In these products, according EU Regulation 2073/2005, is a mandatory criterion irrespective virulence level. Indeed, this encompasses diversity strains with...
The present study reports the results of a preliminary characterization bacterial population Ciauscolo, typical Italian fermented sausage, traditionally manufactured in Marche region. community involved Ciauscolo fermentation was investigated using both molecular and culturebased methods. estimation genotypic intra-species variation autochthonous bacteria isolated also evaluated by randomly amplified polymorphic DNA (RAPD) analysis unweighted pairgroup method with arithmetic averages (UPGMA)...
Salmonella enterica is a major epidemic cause of gastrointestinal infection worldwide. Although the animal host believed to be primary habitat this specie, frequently isolated from water sources and it has been identified in marine environments. In study incidence serotypes coastal Italian region Marche on Adriatic Sea was evaluated. A total 3985 samples molluscan shellfish were analyzed during routine surveillance activity for period five years (2002-2007) 0,95% found contaminated with...
In this study thirty-eight strains of Salmonella enterica serovar Infantis isolated in Marche Region from human cases, food, animal and environmental samples were analyzed by Pulsed-Field Gel Electrophoresis (PFGE). All typed the DNA macro-restriction patterns obtained following PFGE XbaI digests, while antimicrobial susceptibility testing isolates was performed standardized disk diffusion method. The analysis Bionumerics software demonstrated a strong similarity S. infantis profiles,...
The aim of the present study is to investigate operative procedures that allow minimize Listeria monocytogenes (L. m.) hazard in main traditional sausage internal areas Marche (Italy): Ciauscolo, has received quality trademark PGI. It made from lean cuts well mature pork finely minced, adding fat which give salami his characteristic softness and flavour. characterized by having a very little maturing period determine high aw levels and, for this peculiarity, it allows L. m development.