José Luis Spinoso-Castillo

ORCID: 0000-0003-4250-3485
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About
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Research Areas
  • Plant tissue culture and regeneration
  • Botanical Research and Applications
  • Biochemical and biochemical processes
  • Coffee research and impacts
  • Growth and nutrition in plants
  • Mycorrhizal Fungi and Plant Interactions
  • Sugarcane Cultivation and Processing
  • Plant Pathogens and Fungal Diseases
  • Cocoa and Sweet Potato Agronomy
  • Seed Germination and Physiology
  • Medicinal Plants and Neuroprotection
  • Plant Genetic and Mutation Studies
  • Plant responses to water stress
  • Plant Virus Research Studies
  • Allelopathy and phytotoxic interactions
  • Transgenic Plants and Applications
  • Nanoparticles: synthesis and applications
  • Rice Cultivation and Yield Improvement
  • Animal Disease Management and Epidemiology
  • Plant Disease Management Techniques
  • Banana Cultivation and Research
  • Meat and Animal Product Quality
  • Essential Oils and Antimicrobial Activity
  • Plant Pathogens and Resistance
  • Biochemical Analysis and Sensing Techniques

Colegio de Postgraduados
2017-2025

The symbiotic associations between arbuscular mycorrhizal fungi (AMF) and plants can induce drought stress tolerance. In this study, we evaluated the effect of Glomus intraradices, a fungus, on ex vitro development survival sugarcane plantlets subjected to during acclimatization stage micropropagation. obtained (Saccharum spp. cv Mex 69–290) were inoculated with different doses G. intraradices (0, 100, 200 spores per plantlet) greenhouse acclimatization. Sixty days after inoculation,...

10.3390/plants12030687 article EN cc-by Plants 2023-02-03

Abstract Drought-induced water stress affects the productivity of Vanilla planifolia Jacks. ex Andrews crop. In vitro culture technique is an effective tool for study tolerance mechanisms. This aimed to evaluate morphological, physiological and biochemical response V. under in conditions induced with polyethylene glycol (PEG). regenerated shoots 2 cm length were subjected different concentrations PEG 6000 (0, 1, 3% w/v) using Murashige Skoog semi-solid medium. At 60 days culture, growth...

10.1038/s41598-021-02207-0 article EN cc-by Scientific Reports 2021-11-19

Soil salinity is a problem that affects soil fertility and threatens agri-food crop production worldwide. Biotechnology, through plant micropropagation the use of biofertilizers such as arbuscular mycorrhizal fungi (AMF), an alternative to increase productivity induce tolerance stress in different crops. This study aimed evaluate effect doses fungus

10.3390/plants11131780 article EN cc-by Plants 2022-07-05

Worldwide demands of Vanilla planifolia lead to finding new options produce large-scale and contaminant-free crops. Particularly, the Mexican Government has classified at risk it subject protection programs since wild species are in danger extinction no more than 30 clones have been found. Nanotechnology could help solve both genetic variability, but toxicological concerns must be solved. In this work, we present first study cytotoxic genotoxic effects promoted by AgNPs plantlets after a...

10.3390/nano8100754 article EN cc-by Nanomaterials 2018-09-25

Carbon nanotubes play an important role in plant biotechnology due to their effects on the growth and differentiation of cells, tissues, organs, whole plants. This study aimed evaluate effect multi-walled carbon (MWCNTs) during vitro multiplication sugarcane (Saccharum spp.) using a temporary immersion system. Morphological characterization MWCNTs was carried out under transmission electron microscope. Different concentrations (0, 50, 100, 200 mg L-1) were added Murashige Skoog liquid...

10.3390/plants10102015 article EN cc-by Plants 2021-09-26

10.1007/s11240-024-02875-6 article EN Plant Cell Tissue and Organ Culture (PCTOC) 2024-10-01

Water stress caused by drought affects the productivity of sugarcane crop. A breeding alternative is selection drought-tolerant cultivars. The objective this study was in vitro screening cultivars tolerant to water using polyethylene glycol (PEG) as a stressing agent. Cultivars (cv) Mex 69-290, CP 72-2086, 79-431 and MOTZMex 92-207 were subjected different concentrations PEG 6000 (0, 3, 6 9% w/v) Murashige Skoog semi-solid culture medium. At 30 days culture, developmental variables dry...

10.3390/agronomy11030598 article EN cc-by Agronomy 2021-03-22

Taro is important for its nutritional content, medicinal use, and bioethanol production. The aim of the present study was to compare different semi-automated bioreactors (SABs) during in vitro multiplication C. esculenta. SABs used were temporary immersion (TIBs), SETIS™ ebb-and-flow bioreactors; semi-solid culture medium as a control treatment. At 30 d culture, developmental variables, determination chlorophyll, stomatal survival percentage acclimatization evaluated. increased shoot rate...

10.3390/plants10051010 article EN cc-by Plants 2021-05-19

In vitro mutagenesis is an alternative to induce genetic variation in vanilla (Vanilla planifolia Jacks. ex Andrews), which characterized by low diversity. The objective of this study was somaclonal V. gamma radiation and detect it using inter-simple sequence repeat (ISSR) molecular markers. Shoots previously established were multiplied Murashige Skoog culture medium supplemented with 2 mg·L−1 BAP (6-benzylaminopurine). Explants irradiated different doses (0, 20, 40, 60, 80 100 Gy) 60Co...

10.3390/horticulturae8060503 article EN cc-by Horticulturae 2022-06-05

10.1007/978-1-0716-2485-2_16 article EN Methods in molecular biology 2022-01-01

The use of agrochemicals has caused environmental problems and toxicity to humans, so natural alternatives for disease control during harvest postharvest have been evaluated. aim this study was evaluate cinnamon essential oil, neem black sapote fruit extract in vitro inhibition fungi isolated from chayote fruit. extracts were applied at 300, 350, 400 ppm Petri dishes the mycelial growth Fusarium oxysporum, solani, Goetrichum sp., Phytophthora capsici evaluated 7 days, percentage per day...

10.3390/plants12091800 article EN cc-by Plants 2023-04-27

Hybrid compounds have a significant impact on agriculture as slow macro- and micronutrient administration systems. This study aimed to evaluate the synthesis effect of hybrid compound Nd(NO3)3@Zn-MOF in different concentrations vitro growth vanilla (Vanilla planifolia Jacks. ex Andrews). A total 13 plantlets per treatment were cultivated test tubes with semi-solid Murashige Skoog (MS) medium without regulators treated 0, 5, 10, 15, 30 mg L−1 Nd(NO3)3@Zn-MOF. After 60 days culture, we...

10.3390/agronomy14091880 article EN cc-by Agronomy 2024-08-23

In vitro conservation and regeneration of potato germplasm is important in breeding programs. The objective this study was to assess the combined effect paclobutrazol (PAC) silver nanoparticles (AgNPs) during reduced-growth their ability regenerate S. tuberosum after treatments. For system, apices were used as explants Murashige Skoog culture media with different combinations PAC (0, 0.5, 1 2 mg L−1) AgNPs 50, 100 200 L−1). At six months culture, plant length, number lateral branches, leaves...

10.3390/horticulturae9060676 article EN cc-by Horticulturae 2023-06-07
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