Tick-borne encephalitis (TBE) is one of the leading and most dangerous human viral neuroinfections in Europe north-eastern Asia. The clinical manifestations include asymptomatic infections, fevers debilitating that might progress into chronic disease or fatal infection. To understand TBE pathology further host nervous systems, three neural cell lines, neuroblastoma, medulloblastoma glioblastoma, were infected with virus (TBEV). susceptibility virus-mediated cytopathic effect, including...

We have evaluated Photorhabdus insect-related protein (Pir) from asymbiotica against dengue vectors. PirAB shows larvicidal activity both Aedes aegypti and albopictus larvae but did not affect the Mesocyclops thermocyclopoides predator. expressed strongest toxicity compared to PirA, PirB, or mixture of PirA plus PirB. Whether presence an enterobacterial repetitive intergenic consensus sequence in PirAB, has any impact on biological control efficacy needs further investigation.

Eight hard tick species were identified among a total of 466 samples collected from vegetation in southern Thailand: Dermacentor compactus (n = 150), D. steini 100), auratus 85), tricuspis 41), Haemaphysalis hystricis 69), H. semermis 3), shimoga 2) and Amblyomma testudinarium 16). In 93 ticks these 8 species, Coxiella bacteria detected via 16 S rRNA, groEL (60-kDa chaperone heat shock protein B) rpoB (β subunit bacterial RNA polymerase) genes. Interestingly, burnetii was for the first time...

Bacteriophages of Wolbachia bacteria have been proposed as a potential transformation tool for genetically modifying mosquito vectors. In this study, we report the presence WO-B class Wolbachia-associated phages among natural populations several hosts. Eighty-eight percent (22/25) Wolbachia-infected species surveyed were found to contain phages. phage orf7 sequence analysis suggested that single strain was in most singly (23/24) or doubly (1/1) mosquitoes. However, infecting Aedes perplexus...

Female Aedes albopictus mosquitoes from natural populations of different geographical regions Thailand were collected and allowed to oviposit determine relative Wolbachia A B densities their offspring (F1) by using real-time quantitative PCR (RTQ-PCR). An important aspect this work is that all the field. Twenty-seven diverse areas (Songkhla, Konkaen, Chantaburi, Kanchanaburi). The range density in F, was 0.007 1250.78 (bacteria-to-host ratio), whereas ranged 0 348.2 ratio). These data are...

We collected a total of 169 adult hard ticks and 120 nymphs from under the leaves plants located along tourist nature trails in ten localities. The results present data examining vector competence different genera presence Rickettsia Anaplasma species. belonged to three genera, Amblyomma, Dermacentor, Haemaphysalis, comprising 11 bacteria were detected at collection sites, while only one site. Phylogenetic analysis revealed new rickettsia genotypes Thailand that closely related tamurae,...

An engorged female Amblyomma helvolum Koch tick was removed from an adult Varanus salvator Laurenti lizard during field collection in Thailand. After using polymerase chain reaction to amplify three genes (16S rDNA, gltA, and OmpA), we discovered the presence of a Rickettsia sp. Spotted Fever Group. Phylogenetic analysis revealed that this is closely related raoultii Mediannikov. Therefore, report herein for first time detection novel Group

Species of the genus Borrelia are causative agents Lyme disease and relapsing fever. is most commonly reported vector-borne in northern hemisphere. However, some parts world borreliosis fever may be caused by novel genotypes. Herein, we report presence a sp. an Amblyomma varanense collected from Python reticulatus.Ticks were snakes, identified to species level examined PCR for spp. flaB 16S rRNA genes. Phylogenetic trees constructed using neighbour-joining method.Three A. ticks P....

Coxiella bacteria were identified from various tick species across the world. Q fever is a zoonotic disease caused by burnetii that most commonly infects variety of mammals. Non-mammalian hosts, such as birds, have also been reported to be infected with pathogenic form "Candidatus avium". This research increases list found Coxiella-like in Thailand. A total 69 ticks collected 27 domestic fowl (Gallus gallus domesticus), 2 jungle gallus) and 3 Siamese firebacks (Lophura diardi) at 10...

Ticks are ectoparasites of vertebrates and vectors various pathogenic microorganisms. In this study, the presence bacteria protozoa was evaluated by PCR DNA sequencing in 233 mammal ticks collected from 8 provinces Thailand. Sequence phylogenetic analyses partial rickettsial ompA, ompB, sca4 Coxiella 16S rRNA, GroEL, rpoB genes clearly revealed, for first time, a co-infection SFG Rickettsia belonging to R. massiliae subgroup Coxiella-like endosymbiont (CLE), Cox-hein, male Haemaphysalis...

The interactions of tick-borne encephalitis virus (TBEV) with mouse macrophages were studied at the electron microscopic level. cultured sensitive to infection TBEV strain Hypr (a highly neuroinvasive and neurovirulent for laboratory mice) produced relatively high titers. However, these macrophage cells remained morphologically inactivated. Viral particles located mainly in ER but also present other exocytic compartments. No production was observed infected attenuated, non-neuroinvasive 263....

Wolbachia bacteria are common cytoplasmic symbionts of insects, mites and filarial nematodes. They can alter the reproduction their hosts. The symbiont could be eliminated, transferred or used through genetic alteration to take advantage remove possible influences on pests and/or natural enemies. Their extensive effects host fitness have made subject growing attention as a potential biocontrol agent. Here, we summarize relations in control disease vectors pests. Furthermore, drawbacks these...

Abstract Three hundred and forty-four tick samples were collected from vegetation at Taksin Maharat National Park, Tak province, northwestern Thailand. They morphologically identified molecularly confirmed by 16S rRNA COI genes as Dermacentor laothaiensis (n = 105), D. steini 139), auratus 100). These ticks examined for the spotted fever group rickettsiae (SFGRs) using PCR DNA sequencing of six genes; 17-kDa, glt A, rRNA, omp B, sca 4. Of these ticks, 6.10% (21/344) gave positive results...