A5029896796- Redox biology and oxidative stress
- Melanoma and MAPK Pathways
- Protein Kinase Regulation and GTPase Signaling
- Receptor Mechanisms and Signaling
- Computational Drug Discovery Methods
- Iron Metabolism and Disorders
- bioluminescence and chemiluminescence research
- Viral Infectious Diseases and Gene Expression in Insects
- Photoreceptor and optogenetics research
- melanin and skin pigmentation
- Advanced Fluorescence Microscopy Techniques
- Retinal Development and Disorders
- Neurobiology and Insect Physiology Research
- Gold and Silver Nanoparticles Synthesis and Applications
- Genetics, Aging, and Longevity in Model Organisms
- Nanoparticles: synthesis and applications
- Environmental Toxicology and Ecotoxicology
- Protein Tyrosine Phosphatases
- Quinazolinone synthesis and applications
- Cellular Mechanics and Interactions
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Sphingolipid Metabolism and Signaling
- Nitric Oxide and Endothelin Effects
- Metal-Catalyzed Oxygenation Mechanisms
- Lung Cancer Treatments and Mutations
Pennsylvania State University
2022-2024
Wake Forest University
2013-2023
Magee-Womens Research Institute
2022-2023
University of California, San Diego
2018-2021
UC San Diego Health System
2018-2020
Universidad Católica Santo Domingo
2020
North Carolina Agricultural and Technical State University
2020
Brigham Young University
2010
A variety of different signals induce specific responses through a common, extracellular-signal regulated kinase (ERK)-dependent cascade. It has been suggested that signaling specificity can be achieved precise temporal regulation ERK activity. Given the wide distrubtion susbtrates across subcellular compartments, it is important to understand how activity temporally at locations. To address this question, we have expanded toolbox Förster Resonance Energy Transfer (FRET)-based biosensors by...
Extracellular signal-regulated kinases 1 and 2 (ERK1/2) are dysregulated in many pervasive diseases. Recently, we discovered that ERK1/2 is oxidized by signal-generated hydrogen peroxide various cell types. Since the putative sites of oxidation lie within or near ERK1/2's ligand-binding surfaces, investigated how ERK2 regulates interactions with model substrates Sub-D Sub-F. These studies revealed undergoes sulfenylation at C159 on its D-recruitment site surface this modification modulates...
Alternate fuel sources are becoming increasingly important as the reserve of fossil fuels decrease. We describe a photosynthesis mimic that is capable extracting electrons from sacrificial electron donors. This model based on bio-photo-catalyst ferritin. Ferritin an iron storage protein naturally sequesters ferrihydrite inside spherical 12 nm shell. Ferrihydrite semi-conductor functions photo-catalyst in aqueous solvents. has been shown to photoreduce Au<sup>3+</sup> form Au(0)...
Insulin is an important regulator of whole-body glucose homeostasis. In insulin sensitive tissues such as muscle and adipose, induces the translocation transporter 4 (GLUT4) to cell membrane, thereby increasing uptake. However, also signals in that are not generally associated with human reproductive endocrine axis, hyperinsulinemia suppresses secretion gonadotropins from gonadotrope cells anterior pituitary, linking dysregulation suboptimal health. mouse, gonadotropes express receptor which...
Gold metal nanoparticles have applications in bio sensing technology, nano-tube formation, and cancer therapy. We report attempts to synthesize gold within the ferritin cavity (8 nm) or use as a scaffold for coating on outside surface (12 nm). The intrinsic iron oxide core of is semi-conductor light can excite electrons conduction band producing powerful reductant when sacrificial electron donor fills hole. present method using photo chemically reduce Au(III) metallic nanoparticles. During...
Reactive oxygen species (ROS), such as hydrogen peroxide (H 2 O ), impact many aspects of cellular physiology. Aside from the incidental generation ROS that occurs during respiration, regulated production by professional ROS‐generating enzymes is now recognized a key step in redox‐dependent signaling pathways. While our understanding mechanisms controlling levels has grown considerably recent years, less known about molecular events occur downstream generation. Oxidation redox‐sensitive Cys...
In order to elicit an appropriate response toward a stimulus, cells have developed intricate systems control signaling pathways. These pathways are often centered on kinase cascades, which modulated by 2 nd messengers and protein‐protein interactions. Such modulation is necessary for the spatial‐temporal of signal exertion. It through these mechanisms that can induce specific core cascade shared other signals different responses. Recently recognized Reactive Oxygen Species (ROS) such as H O...
Abstract A variety of different signals induce specific responses through a common, ERK-dependent kinase cascade. It has been suggested that signaling specificity can be achieved precise temporal regulation ERK activity. Given the wide distrubtion susbtrates across subcellular compartments, it is important to understand how activity temporally regulated at locations. To address this question, we have expanded toolbox FRET-based biosensors by creating series improved targeted various regions...
The ERK1/2 pathway plays critical roles in eukaryotic biology by transducing extracellular signals into cell‐fate decisions. One conundrum is understanding how disparate induce specific responses through a common, ERK‐dependent kinase cascade. major mechanism to control ERK‐signaling proper cellular precise regulation over the temporal dynamics of ERK activity. For example, pheochromocytoma PC‐12 cell line, exhibits transient activity response Epidermal Growth Factor (EGF), ultimately...
A variety of different signals induce specific responses through a common, ERK‐dependent kinase cascade. It has been suggested that signaling specificity can be achieved precise temporal regulation ERK activity. Given the wide distrubution susbtrates across subcellular compartments, it is important to understand how activity temporally regulated at locations. To address this question, we expanded toolbox FRET‐based biosensors by creating series improved targeted various regions via sequence...
G‐protein coupled receptors (GPCRs) play vital roles in regulating physiological functions, such as sympathetic nervous regulation, and senses of vision olfaction. Signaling the β2‐adrenergic receptor (β2‐AR), which is a prototypical class A GPCR, has been proposed to control activation ERK, subsequently different processes including gene expression. However, molecular mechanism ERK downstream β2AR signaling not clearly understood. Here we investigate β2‐AR mediated using improved version...
Cellular processes controlling proliferation, migration, differentiation and adherence require signal transduction processes. Subcellular compartmentalization of signaling proteins controls their temporal spatial activity, can be assessed by subcellular fractionation. However, most fractionation protocols are not effective for certain cell lines as the integrity samples produced often compromised cross‐contamination. Here, we report a newly optimized protocol that yields clean cytoplasmic,...
Mitogen activated protein kinases (MAPKs) are by various stimuli, ranging from growth factors to environmental stress factors. However, given this diverse set of it is not currently clear how specificity achieved in MAPK signaling pathways. We exploring the hypothesis that, modulating substrate selectivity family members, cells able control which “arm” a branched pathway response signal. Specifically, we investigating impact redox modification on global selection canonical MAPKs,...