A5002590983- Iron Metabolism and Disorders
- Trace Elements in Health
- Hemoglobinopathies and Related Disorders
- Iron oxide chemistry and applications
- Quantum Dots Synthesis And Properties
- Gold and Silver Nanoparticles Synthesis and Applications
- Advanced biosensing and bioanalysis techniques
- Plant Micronutrient Interactions and Effects
- Photosynthetic Processes and Mechanisms
- Chalcogenide Semiconductor Thin Films
- Porphyrin Metabolism and Disorders
- Nanoparticles: synthesis and applications
- Metalloenzymes and iron-sulfur proteins
- Advanced Photocatalysis Techniques
- Electrochemical Analysis and Applications
- Metal Extraction and Bioleaching
- Microbial metabolism and enzyme function
- Photoreceptor and optogenetics research
- Erythropoietin and Anemia Treatment
- Mitochondrial Function and Pathology
- Heme Oxygenase-1 and Carbon Monoxide
- Corrosion Behavior and Inhibition
- Alzheimer's disease research and treatments
- Nanocluster Synthesis and Applications
- Spectroscopy and Quantum Chemical Studies
Brigham Young University
2012-2024
Roseman University of Health Sciences
2016
Provo College
2014
University of Utah
2007
GE Global Research (United States)
2007
Princeton University
1999-2006
Carnegie Mellon University
2006
University of New Mexico
2004-2005
University of Wisconsin–Madison
1996-1999
California Polytechnic State University
1999
Nanotubes prepared by the self-assembly of D-Phe-D-Phe molecules are investigated electron microscopy and Monte Carlo simulations; nanotubes appear to be porous capable forming novel peptide–nanotube platinum–nanoparticle composites.
Development of pharmacotherapies that promote remyelination is a high priority for multiple sclerosis (MS), due to their potential neuroprotection and restoration function through repair demyelinated lesions. A novel preparation clean-surfaced, faceted gold nanocrystals demonstrated robust remyelinating activity in response demyelinating agents both chronic cuprizone acute lysolecithin rodent animal models. Furthermore, oral delivery improved motor functions cuprizone-treated mice open field...
An electron microscopy study, in combination with modeling and image simulation, of four different reconstituted ferritin samples: recombinant human H L homopolymers, heteropolymers native L-subunit-rich horse spleen H-subunit-rich heart ferritins, points out the existence a correlation between iron core shape protein shell.
Nanoparticles of iron phosphate, arsenate, molybdate, and vanadate were synthesized within the 8 nm interior ferritin. The synthesis involved reacting Fe(II) with ferritin in a buffered solution at pH 7.4 presence vanadate, or molybdate. O2 was used as oxidant to deposit Fe(III) mineral inside rate incorporation into stimulated when oxo-anions present. simultaneous deposition both oxo-anion confirmed by elemental analysis energy-dispersive X-ray analysis. samples containing one possessed...
CooJ, a nickel-binding protein from the CO dehydrogenase system of Rhodospirillum rubrum, was purified by immobilized metal affinity chromatography. CooJ is CO-induced predicted to contain nickel binding motif composed 16 histidine residues in final 34 amino acids 12.5-kDa protein. When cells grown presence were fractionated on an chromatography column and analyzed SDS-polyacrylamide gel electrophoresis, major observed effluent migrated at apparent molecular mass 19 kDa. The 19-kDa absent...
Biogenesis and repair of the inorganic core (Mn4CaO(x)Cl(y)), in water-oxidizing complex photosystem II (WOC-PSII), occurs through light-induced (re)assembly its free elementary ions apo-WOC-PSII protein, a reaction known as photoactivation. Herein, we use electron paramagnetic resonance (EPR) spectroscopy to characterize changes ligand coordination environment first photoactivation intermediate, photo-oxidized Mn3+ bound apo-WOC-PSII. On basis observed Zeeman (g(eff)), 55Mn hyperfine (A(Z))...
Protein arrays continue to increase in importance as tools for analysis of biological samples. This paper describes a new method preparing bioarrays that is compatible with high throughput manufacturing. First, native oxide terminated silicon substrate coated monolayer polyethylene-glycol-containing silane. The then placed beneath microlens array (MA) and the irradiated brief (4 ns) pulse 532 nm laser light. MA focuses light onto substrate, causing removal. microlenses employed this work are...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRedox reactions of apo mammalian ferritinR. K. Watt, R. B. Frankel, and G. D. WattCite this: Biochemistry 1992, 31, 40, 9673–9679Publication Date (Print):October 13, 1992Publication History Published online1 May 2002Published inissue 13 October 1992https://pubs.acs.org/doi/10.1021/bi00155a021https://doi.org/10.1021/bi00155a021research-articleACS PublicationsRequest reuse permissionsArticle Views263Altmetric-Citations43LEARN ABOUT THESE...
Ferritin (Ft) interaction with the Zn-complexes of mammalian MT1, MT2 and MT3 metallothioneins (MT) leads to simultaneous FeII ZnII release.
Isolated subcomplexes of photosystem II from spinach (CP47RC), composed D1, D2, cytochrome b 559 , CP47, and a number hydrophobic small subunits but devoid CP43 the extrinsic proteins oxygen-evolving complex, were shown to reconstitute Mn 4 Ca 1 Cl x cluster water-splitting system evolve oxygen. The photoactivation process in CP47RC dimers proceeds by same two-step mechanism as observed PSII membranes exhibits stoichiometry for 2+ with 10-fold lower affinity an increased susceptibility...
ABSTRACT The cooCTJ gene products are coexpressed with CO-dehydrogenase (CODH) and facilitate in vivo nickel insertion into CODH. A Ni 2+ transport assay was used to monitor uptake accumulation of 63 R. rubrum observe the effect mutations cooC , cooT cooJ genes on accumulation. Cells grown either presence or absence CO transported a K m 19 ± 4 μM V max 310 22 pmol Ni/min/mg total protein. Insertional disrupting reading frame genes, individually all three simultaneously, same as wild-type...
Ferritin is a protein nano-cage that encapsulates minerals inside an 8 nm cavity. Previous band gap measurements on the native mineral, ferrihydrite, have reported gaps as low 1.0 eV and high 2.5-3.5 eV. To resolve this discrepancy we used optical absorption spectroscopy, well-established technique for measuring both direct indirect gaps. Our studies included controls nano-cage, ferritin with ferrihydrite reconstituted cores of different sizes. We report 2.140 ± 0.015 (579.7 nm), transition...
We previously reported that ferritin acts as a photocatalyst to form monodispersed gold nanoparticles. Because silver possesses favorable reduction potential (+0.80 V), we postulated the photochemical method could be used reduce Ag(I) Ag(0). Additionally, similar gold, reduced should nucleate and nanoparticles (AgNPs) on external surface of ferritin. This study reports can function an electron acceptor AgNPs using photocatalytic method. The formation growth were monitored by following...
Doxorubicin (DOX) is an effective chemotherapeutic treatment with lasting side effects in heart and skeletal muscle. DOX known to bind iron, contributing oxidative damage resulting cardiac muscle toxicity. However, major cellular changes iron regulation response are poorly understood liver, heart, Additionally, two cotreatments, exercise (EX) metformin (MET), were studied for their effectiveness reducing toxicity by ameliorating dysregulation preventing stress. The purposes of this study 1)...
Ironing out the differences: A unified model shows that catalytic centers of human H ferritin and archaeal P. furiosus load iron according to same mechanism. This could help our understanding processes controlling various subcellular concentrations during inflammation.
Parkinson's disease is associated with degeneration of neuromelanin (NM)-containing substantia nigra dopamine (DA) neurons and subsequent decreases in striatal DA transmission. Dopamine spontaneously forms a melanin through process called melanogenesis. The present study examines conditions that promote/prevent kinetics, intermediates, products conversion to vitro, melanogenesis under varying levels Fe3+, pro-oxidants, antioxidants were examined. rate for was substantially greater than...
Apo horse spleen ferritin (apo HoSF) was reconstituted to various core sizes (100−3500 Fe3+/HoSF) by depositing Fe(OH)3 within the hollow HoSF interior air oxidation of Fe2+. Fe2+ and phosphate (Pi) were then added anaerobically at a 1:4 ratio, both Pi incorporated into cores. The resulting layer consisted about 1:3 ratio which is strongly attached mineral surface stable even after bound total amount iron increases as volume up maximum near 2500 atoms, above size decreases with increasing...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRole of phosphate in iron(2+) binding to horse spleen holoferritinHeqing Huang, Richard K. Watt, B. Frankel, and Gerald D. WattCite this: Biochemistry 1993, 32, 6, 1681–1687Publication Date (Print):February 1, 1993Publication History Published online1 May 2002Published inissue 1 February 1993https://pubs.acs.org/doi/10.1021/bi00057a036https://doi.org/10.1021/bi00057a036research-articleACS PublicationsRequest reuse permissionsArticle...