A5025007020- Advanced biosensing and bioanalysis techniques
- Ion channel regulation and function
- Biosensors and Analytical Detection
- Cerebrospinal fluid and hydrocephalus
- bioluminescence and chemiluminescence research
- Analytical Chemistry and Sensors
- Neonatal Respiratory Health Research
- ATP Synthase and ATPases Research
- Bacterial Genetics and Biotechnology
- Trace Elements in Health
- Fetal and Pediatric Neurological Disorders
- Ion Transport and Channel Regulation
- Nitrogen and Sulfur Effects on Brassica
- DNA and Nucleic Acid Chemistry
- Glycosylation and Glycoproteins Research
Westlake University
2021-2024
Institute of Basic Medical Sciences of the Chinese Academy of Medical Sciences
2021-2022
Abstract The glymphatic movement of fluid through the brain removes metabolic waste 1–4 . Noninvasive 40 Hz stimulation promotes neural activity in multiple regions and attenuates pathology mouse models Alzheimer’s disease 5–8 Here we show that multisensory gamma influx cerebrospinal efflux interstitial cortex 5XFAD model disease. Influx was associated with increased aquaporin-4 polarization along astrocytic endfeet dilated meningeal lymphatic vessels. Inhibiting clearance abolished removal...
Genetically encoded potassium indicators lack optimal binding affinity for monitoring intracellular dynamics in mammalian cells. Through structure-guided design and genome mining of proteins, we developed green fluorescent with a broad range affinities. KRaION1 (K+ ratiometric indicator optical imaging based on mNeonGreen 1), the insertion protein, Kbp, from E. coli (Ec-Kbp) into protein mNeonGreen, exhibits an isotonically measured Kd 69 ± 10 mM (mean standard deviation used throughout). We...
Engineered light, oxygen, and voltage (LOV)-based proteins are able to fluoresce without oxygen requirement due the autocatalytic incorporation of exogenous flavin as a chromophore thus allowing for live cell imaging under hypoxic anaerobic conditions. They were also discovered have high sensitivity transition metal ions physiological derivatives. These properties make flavin-binding fluorescent (FPs) perspective platform biosensor development. However, brightness currently available FPs is...
Abstract Genetically encoded fluorescent biosensors became indispensable tools for biological research enabling real-time observation of physiological processes in live cells. Recent protein engineering effort has resulted generation large variety wide range biologically relevant from small ions to enzymatic activity and signaling pathways. However, imaging sulfate ion, the fourth most abundant anion, mammalian cells are still lacking. Here we report development characterization green...
Genetically encoded fluorescent biosensors became indispensable tools for biological research, enabling real-time observation of physiological processes in live cells. Recent protein engineering efforts have resulted the generation a large variety wide range biologically relevant processes, from small ions to enzymatic activity and signaling pathways. However, imaging sulfate ions, fourth most abundant anion, mammalian cells are still lacking. Here, we report development characterization...
Potassium ion (K+) dynamics are vital for various biological processes. However, the limited availability of detection tools tracking intracellular and extracellular K+ has impeded a comprehensive understanding physiological roles in intact systems. In this study, we developed two novel red genetically encoded potassium indicators (RGEPOs), RGEPO1 RGEPO2, through combination directed evolution E. coli subsequent optimization mammalian cells. RGEPO1, targeted to membrane, localized cytoplasm,...
Abstract Genetically encoded potassium indicators lack optimal binding affinity for monitoring intracellular dynamics in mammalian cells. Through structure-guided design and genome mining of proteins, we developed green fluorescent with a broad range affinities. KRaION1, based on the insertion protein (Ec-Kbp) into mNeonGreen, exhibits an isotonically measured K d 69±10 (mM; mean ± standard deviation used throughout). We identified Ec-Kbp’s site using NMR spectroscopy to detect...