A5044742144- Advanced biosensing and bioanalysis techniques
- Antioxidant Activity and Oxidative Stress
- Nitrogen and Sulfur Effects on Brassica
- Analytical Chemistry and Sensors
- Algal biology and biofuel production
- Microbial Metabolism and Applications
- Photosynthetic Processes and Mechanisms
- Glycosylation and Glycoproteins Research
- ATP Synthase and ATPases Research
Westlake University
2024
Tianjin University
2017
The diverse spectral and photochemical properties of fluorescent proteins enable a variety imaging applications in cell biology, ranging from cellular organelle labeling to super-resolution microscopy multiplexed live imaging. Here, we report set novel far-red proteins, named mfRFP, mfRFP-A, mCardinal-A, which are characterized by similar fluorescence spectra with excitation/emission at ~600/660 nm while exhibiting distinct photobleaching rates. Differences photostability allowed us perform...
Abstract Genetically encoded fluorescent biosensors became indispensable tools for biological research enabling real-time observation of physiological processes in live cells. Recent protein engineering effort has resulted generation large variety wide range biologically relevant from small ions to enzymatic activity and signaling pathways. However, imaging sulfate ion, the fourth most abundant anion, mammalian cells are still lacking. Here we report development characterization green...
Genetically encoded fluorescent biosensors became indispensable tools for biological research, enabling real-time observation of physiological processes in live cells. Recent protein engineering efforts have resulted the generation a large variety wide range biologically relevant processes, from small ions to enzymatic activity and signaling pathways. However, imaging sulfate ions, fourth most abundant anion, mammalian cells are still lacking. Here, we report development characterization...