A5100750394- Analytical Chemistry and Chromatography
- Protein purification and stability
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Neuroscience and Neural Engineering
- Microfluidic and Capillary Electrophoresis Applications
- Cholinesterase and Neurodegenerative Diseases
- Chemical Reaction Mechanisms
- Neuroscience and Neuropharmacology Research
- Glycosylation and Glycoproteins Research
- Neurological disorders and treatments
- Colorectal Cancer Treatments and Studies
- Pediatric Pain Management Techniques
- Chronic Myeloid Leukemia Treatments
- Chromatography in Natural Products
- Chronic Lymphocytic Leukemia Research
- Lung Cancer Treatments and Mutations
- Salivary Gland Disorders and Functions
- Metabolomics and Mass Spectrometry Studies
- Nicotinic Acetylcholine Receptors Study
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Vestibular and auditory disorders
- Carbohydrate Chemistry and Synthesis
- Hearing, Cochlea, Tinnitus, Genetics
- Viral Infections and Outbreaks Research
University of Alabama at Birmingham
2022-2023
Vanderbilt University
2019-2023
Cleveland State University
2002-2023
Imaging Center
2019-2023
Istituto Tecnico Industriale Alessandro Volta
2021
Weatherford College
2021
St. Louis County Missouri
2016
NeuroNexus (United States)
2010-2015
Huntsman Cancer Institute
2011-2014
University of Utah
2011-2014
The interface between micromachined neural microelectrodes and tissue plays an important role in chronic vivo recording. Electrochemical polymerization was used to optimize the surface of metal electrode sites. Electrically conductive polymers (polypyrrole) combined with biomolecules having cell adhesion functionality were deposited great precision onto microelectrode sites probes. a silk-like polymer fibronectin fragments (SLPF) nonapeptide CDPGYIGSR. existence protein peptides coatings...
We describe the combined use of 15N-metabolic labeling and a cysteine-reactive biotin affinity tag to isolate quantitate cysteine-containing polypeptides (Cys-polypeptides) from Deinococcus radiodurans as well mouse B16 melanoma cells. D. were cultured in both natural isotopic abundance 15N-enriched media. Equal numbers cells cultures soluble proteins extracted. This mixture isotopically distinct was derivatized using commercially available reagent that contains group. Following trypsin...
Recent evidence indicating the therapeutic potential of cholinergic channel modulators for treatment central nervous system (CNS) disorders as well diversity brain neuronal nicotinic acetylcholine receptors (nAChRs) have suggested an opportunity to develop subtype-selective nAChR ligands specific CNS with reduced side effect liabilities. We report a novel series 3-pyridyl ether compounds which possess subnanomolar affinity nAChRs and differentially activate subtypes nAChRs. The synthesis...
Challenges associated with the efficient and effective preparation of micro- nanoscale (micro- nanogram) clinical specimens for proteomic applications include unmitigated sample losses that occur during processing steps. Herein, we describe a simple “single-tube” protocol appropriate small samples using organic cosolvent, trifluoroethanol (TFE) circumvents loss by facilitating both protein extraction denaturation without requiring separate cleanup step. The performance TFE-based method was...
Identification of novel diagnostic or therapeutic biomarkers from human blood plasma would benefit significantly quantitative measurements the proteome constituents over a range physiological conditions. Herein we describe an initial demonstration proteome-wide analysis plasma. The approach utilizes postdigestion trypsin-catalyzed 16O/18O peptide labeling, two-dimensional LC-FTICR mass spectrometry, and accurate time (AMT) tag strategy to identify quantify peptides/proteins complex samples....
Recently, cyclooctylpyranone derivatives with m-carboxamide substituents (e.g. 2c) were identified as potent, nonpeptidic HIV protease inhibitors, but these compounds lacked significant antiviral activity in cell culture. Substitution of a sulfonamide group at the meta position, however, produces excellent binding affinity and activity. Guided by an iterative structure-based drug design process, we have prepared evaluated number derivatives, which are readily available via seven-step...
Salivary flow has been recorded from conscious rabbits during 1 min periods whilst continuously chewing standard laboratory dry pellets or pieces of carrot and, in some animals, a mash with water. Flow was measured using contact drop recorders continuous recorder via Polythene tubes permanently inserted into one both parotid ducts. Large variations were obtained unilateral recordings particularly pellet chewing. Bilateral showed that the always greater on side than other and dominant...
Temporally and spatially resolved mapping of protein abundance patterns within the mammalian brain is significant interest for understanding function molecular etiologies neurodegenerative diseases; however, such imaging efforts have been greatly challenged by complexity proteome, throughput sensitivity applied analytical methodologies, accurate quantitation abundances across brain. Here, we describe a methodology comprehensive spatial proteome that addresses these challenges employing...
Automated multidimensional capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been increasingly applied in various large scale proteome profiling efforts. However, comprehensive global analysis remains technically challenging due to issues associated with sample complexity and dynamic range of protein abundances, which is particularly apparent mammalian biological systems. We report here the application a high efficiency cysteinyl peptide enrichment (CPE) approach human...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTChemistry of small ring heterocycles. XVIII. 3-H-Azepines from azirines and cyclopentadienonesAlfred Hassner David J. AndersonCite this: Am. Chem. Soc. 1971, 93, 17, 4339–4340Publication Date (Print):August 1, 1971Publication History Published online1 May 2002Published inissue 1 August 1971https://pubs.acs.org/doi/10.1021/ja00746a066https://doi.org/10.1021/ja00746a066research-articleACS PublicationsRequest reuse permissionsArticle...
Develop and characterize the functionality of a novel thin-film probe technology with higher density electrode contacts than are currently available commercial deep brain stimulation (DBS) lead technology. Such has potential to enhance spatial precision DBS enable more robust approach sensing local field activity in context adaptive strategies.Thin-film planar arrays were microfabricated then assembled on cylindrical carrier achieve 3-D conformation. Using an integrated removable stylet,...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHigh-Performance Liquid Chromatography (Direct Injection Techniques)David J. AndersonCite this: Anal. Chem. 1993, 65, 12, 434–443Publication Date (Print):June 15, 1993Publication History Published online5 March 2003Published inissue 15 June 1993https://pubs.acs.org/doi/10.1021/ac00060a613https://doi.org/10.1021/ac00060a613research-articleACS PublicationsRequest reuse permissionsArticle Views405Altmetric-Citations57LEARN ABOUT THESE METRICSArticle...
A new chromatofocusing technique called gradient is characterized. Gradient generates linear pH gradients on anion-exchange columns with inexpensive low molecular mass buffer components via HPLC mixing. results are compared that of conventional in the chromatography several proteins a Mono P column, including β-lactoglobulin and B, ovalbumin, BSA, conalbumin. shows superior performance, resolution increases greater than 3-fold being realized for entire protein mixture up to 25-fold...
Gabapentin and pregabalin have been demonstrated, both in animal pain models clinically, to be effective analgesics particularly for the treatment of neuropathic pain. The precise mechanism action these two drugs is unknown, but they are generally believed function via initially binding alpha2delta subunit voltage-gated Ca2+ channels. In this study, we used a pharmacological approach test hypothesis whether high affinity interactions with alone could lead attenuation rats. anti-allodynic...