LncRNAs have become a hot topic in various cancer-related researches. Radio-resistance is great threat for cancer therapy. However, how lncRNAs affect the radio-resistance cervical masked. As our paper, it was discovered that NEAT1 highly expressed tissues and non-sensitive as well radio-resistant cell lines. And overexpression of accelerated proliferation, while knockdown had opposite result. The effect on proliferation dependent dose ionizing radiation. silence also caused cycle arrest...

Litchi (Litchi chinensis Sonn.) fruit deterioration occurs rapidly after harvest and is characterized by pericarp browning, pulp softening, decay. In this study, we found that calcium chloride (CaCl2) treatment (5 g L-1 CaCl2 solution vacuum infiltration for 5 min) affected the cell wall component contents wall-degrading enzyme activities of litchi during storage at room temperature. significantly increased Ca2+ cellulose, while it decreased water-soluble pectin content, polygalacturonase,...

Early identification of esophageal cancer patients who are responding or resistant to combined chemoradiotherapy may lead individualized therapeutic approaches and improved clinical outcomes. We assessed the ability 3'-deoxy-3'-(18)F-fluorothymidine positron emission tomography (FLT-PET) detect early changes in tumor proliferation after experimental models carcinoma.The vitro ex vivo uptake [(3)H]FLT SEG-1 human adenocarcinoma cells were studied at various time points docetaxel plus...

Taking the emergence of drug resistance and lack effective antimalarial vaccines into consideration, it is significant importance to develop novel agents for treatment malaria. Herein, we elucidated discovery structure-activity relationships a series dihydrothiophenone derivatives as specific inhibitors Plasmodium falciparum dihydroorotate dehydrogenase (PfDHODH). The most promising compound, 50, selectively inhibited PfDHODH (IC50 = 6 nM, with >14,000-fold species-selectivity over hDHODH)...

Thermotoga spp. are attractive candidates for producing biohydrogen, green chemicals, and thermostable enzymes. They may also serve as model systems understanding life sustainability under hyperthermophilic conditions. A lack of genetic tools has hampered the investigation application these organisms. This study aims to develop a transfer system spp.Methods preparing handling solid cultures aerobic conditions were optimized. plating efficiency ~50% was achieved when bacterial cells embedded...

Thermotoga species are organisms of enormous interest from a biotechnological as well evolutionary point view. Genetic modifications spp. often desired in order to fully release their multifarious potentials. Effective transformation recombinant DNA into these bacteria constitutes critical step such efforts. This study aims establish natural competency and provide convenient method transform organisms. Foreign was found be relatively stable the supernatant culture for up 6 hours. Adding...

Gibberellic acids had been proven to improve the fruit quality and storability by delaying deterioration maintaining antioxidant system. In this study, effect of GA3 spraying at different concentrations (10, 20, 50 mg L−1) on on-tree preserved ‘Shixia’ longan was examined. Only L−1 significantly delayed decline soluble solids (22.0% higher than control) resulted in total phenolics content (TPC), flavonoid (TFC), phenylalanine ammonia-lyase activity pulp later stages. The widely targeted...

R2R3-MYB is an important transcription factor family that regulates plant growth and development. Root development directly affects the absorption of water nutrients by plants. Therefore, to understand regulatory role in root longan, this study identified gene members at genome-wide level, analyzed their phylogenetic characteristics, physical chemical properties, structure, chromosome location tissue expression. The analysis 124 longan genome. Phylogenetic divided these into 22 subfamilies,...

The ability of Thermotoga spp. to degrade cellulose is limited due a lack exoglucanases. To address this deficiency, cellulase genes Csac_1076 ( celA ) and Csac_1078 celB from Caldicellulosiruptor saccharolyticus were cloned into T. sp. strain RQ2 for heterologous overexpression. Coding regions fused the signal peptide TM1840 amyA TM0070 xynB ), resulting in three chimeric enzymes, namely, TM1840-Csac_1078, TM0070-Csac_1078, TM0070-Csac_1076, which carried by Thermotoga-E. coli shuttle...

Longan fruit pulp sugar content and sweetness decline after ripening, which affects quality postharvest storage. Fruit research has focused mainly on physiology biochemistry. To explore the molecular mechanism underlying decline, we conducted transcriptome metabolome analyses of 'Shixia' longan aril (pulp) tissues at different stages development. In total, 873 metabolites 26584 annotated unigenes were identified. Sugar changes in closely related to gene expression patterns starch sucrose...

Soluble acid invertases (SAIs) cleave sucrose into hexose in vacuoles and play important roles influencing fruit quality. However, their potential regulating sugar composition the "sugar receding" process of longan fruits lacked systematic investigations. Our results showed that sucrose/hexose ratios receding rates pulp varied among cultivars. Analysis enzymes for synthesis cleavage indicated DlSAI highest negative correlation with ratio at both activity expression level. Moreover, high SAI...

Studies on how temperature affects the postharvest quality of litchi have focused mainly pericarp browning but rarely metabolites in pulp. In this study, differences respiration rates, total soluble solid content, and titratable acid content demonstrated that room low temperatures different effects "Feizixiao" UHPLC-ESI-QTOF-MS/MS analysis was performed to compare differentially expressed (DEMs) pulp after 8 days storage at (RT-8 d) with those 28 (LT-28 d). Nineteen carbohydrates...

The residual sulfite caused by sulfur fumigation (SF) is a hazard to health and influenced the export trade of litchi. Desulfurization (DS) valid chemical method reduce sulfite. However, effect DS on fumigated litchi has not been studied at physiological molecular level. This study was aimed evaluate (SF plus 3% desulfurizer) postharvest quality, residue, metabolism in sulfitated "Feizixiao" during 4°C storage. Results indicated that promoted color recovery achieved an similar SF controlling...

Thermotoga sp. strain RQ7 is a member of the family Thermotogaceae in order Thermotogales. It Gram negative, hyperthermophilic, and strictly anaerobic bacterium. grows on diverse simple complex carbohydrates can use protons as final electron acceptor. Its complete genome composed chromosome 1,851,618 bp plasmid 846 bp. The contains 1906 putative genes, including 1853 protein coding genes 53 RNA genes. genetic features pertaining to various lateral gene transfer mechanisms are analyzed....

Zinc (Zn) fertilization has been reported to improve the quality and storability of many fruits, but mechanism had not systematically studied. In this study, effect preharvest 0.2% zinc sulfate (ZnSO4 ) spray on longan fruits was investigated.The ZnSO4 did significantly influence increased Zn content in pericarp by 12.5-fold. More importantly, treatment reduced rotting rate, browning, aril breakdown stored at room temperature low temperature. Physiological biochemical results indicated that...

Longan was a characteristic fruit for both medicine and food in China, which rich primary secondary metabolites. Comprehensive high-throughput identification comparison of metabolites longan pulp among different varieties were still lacked. "Shixia" (SX) "Chuliang" (CL) the biggest major cultivars China. In this study, content total soluble solid, flavonoid, phenolics indicated difference sweetness bioactive compound between SX CL pulp. Through widely targeted metabolome, 514 identified...