Abstract Objective To evaluate infliximab efficacy and safety in disease‐modifying antirheumatic drug–unresponsive psoriatic arthritis (PsA). Methods In a 54‐week, open‐label, compassionate‐use study, 10 patients received intravenous (5 mg/kg; weeks 0, 2, 6; individualized dosing after week 10). Patients continued their current therapy (stable dose) until 10. Assessments were performed at 6, 10, 54. Magnetic resonance imaging (MRI) objectively measured joint inflammation 0 Results achieved...

The conidium plays a critical role in the life cycle of many filamentous fungi, being primary means for survival under unfavorable conditions. To investigate transcriptional changes taking place during transition from growing hyphae to conidia Trichoderma reesei, microarray experiments were performed. A total 900 distinct genes classified as differentially expressed, relative their expression at time zero conidiation, least one points analyzed. main functional categories (FunCat)...

Abstract To date, commercial laccase preparations are used in the food, textile, and paper pulp industries (mild pH). Laccases attractive synthesis of dye molecules or oxidative lignin treatment, which take place at high pH (≥8.0). So far, one fungal has been reported to be active alkaline pH. Herein, engineering from Melanocarpus albomyces (MaL) for increased activity toward substrate 2,6‐dimethoxyphenol (≥9.0) is reported. Through a knowledge‐gaining directed evolution (KnowVolution)...

The coding sequence of a peroxidase from the secretome Pleurotus sapidus was cloned cDNA library. Bioinformatic analyses revealed an open reading frame 1551 bp corresponding to primary translation product 516 amino acids. DyP-type heterologously produced in Trichoderma reesei with activity 55,000 U L-1. enzyme purified culture supernatant, biochemically characterized and kinetic parameters were determined. has N-terminal signal peptide composed 62 Analysis by Blue Native PAGE staining ABTS,...

Filamentous fungi are frequently used as production platforms in industrial biotechnology. Most of the strains involved were known reproducing exclusively asexually thereby preventing application conventional strain breeding techniques. In last decade, evidence was obtained that a number these imperfect possess sexual life cycle, too. Trichoderma reesei, an producer enzymes for food, feed and biorefinery purposes, is heterothallic takes special position among industrially utilized species...

Bacillus subtilis is an important cell factory for the biotechnological industry due to its ability secrete commercially relevant proteins in large amounts directly into growth medium. However, hyper-secretion of proteins, such as α-amylases, leads induction secretion stress-responsive CssR-CssS regulatory system, resulting up-regulation HtrA and HtrB proteases. These proteases degrade misfolded secreted via Sec pathway, a loss product. The aim this study was investigate stress response B....

Abstract We report a novel, inexpensive and effective process for the repeatable photoisomerization of norbornadiene (NBD) to its metastable isomer quadricyclane (QC), followed by catalytically induced strain energy release via back‐conversion QC NBD. By utilization quasi‐homogeneous catalyst based on magnetic core‐shell nanoparticles, tedious purification steps are avoided. The core this material is comprised Fe 3 O 4 active cobalt(II) complex anchored particle surface as self‐assembled...

Cobalt catalysts are immobilized on the surface of iron oxide nanoparticles for preparation highly active quasi-homogeneous toward an efficient release photochemically stored energy in norbornadiene-based photoswitches. The facile separation through exploitation intrinsic magnetic properties this material enables cyclization storage and release. Through transition from cobalt (II) salphen to porphyrins, a 22.6-fold increase catalytic efficiency QC-NBD back-conversion is achieved, with...

We describe for the first time full reaction coordinate regarding photoisomerization of red-absorbing norbornadienes (NBDs) to quadricyclanes (QCs). Our studies go beyond steady-state investigations by using an arsenal time-resolved techniques. Importantly, red absorption NBDs is made possible a different charge-transfer character; adjusting its strength enables control over photoreversibility rearrangement. In case strong character (a weakly electron-withdrawing ester and strongly...

The synthesis and properties of various norbornadiene/quadricyclane (NBD/QC) fullerene hybrids are reported. By cyclopropanation C60 with malonates carrying the NBD scaffold a small library NBD-fullerene monoadducts hexakisadducts was established. substitution pattern scaffold, as well electron affinity core within these hybrid systems, has pronounced impact on corresponding energy rich QC derivatives. Based this, first direct photoisomerization to their derivatives achieved. Furthermore, it...

Kinetoplastid parasites compartmentalize the first seven enzymes of glycolysis in a peroxisome‐like microbody, glycosome. Genes encoding most abundant protein glycosomal membrane, GIM5, have been cloned and characterized. Two genes, GIM5A GIM5B , encode 26 kDa proteins. Although many microbody membrane proteins are conserved evolution, only homologues GIM5 available databases from closely related kinetoplastids Trypanosoma cruzi Leishmania . The N‐ C‐termini between two species, oriented...

Abstract A novel Dye-decolorizing peroxidase from the basidiomycete Pleurotus sapidus was screened for dyedecolorizing activity with 2,2‘-azino-bis(3- ethylbenzothiazoline-6-sulfonic acid), Remazol Brilliant Blue R and Guaiacol. Additionally, catalytic efficiency on degrading β-carotene into volatile products, catalyst storage stability three different additives were also studied. The apparent inhibition constant (KS) 51.7 μM. Optimal reaction rates (Vmax) affinity constants (Km) towards...

Journal Article Importance of acidic intracellular compartments in the lysis Trypanosoma brucei by normal human serum Get access Patrick Lorenz, Lorenz Swiss Tropical Institute, Postfach, CH-4002 Basel, Switzerland Search for other works this author on: Oxford Academic PubMed Google Scholar Philip E. Barth, Barth Werner Rudin, Rudin Bruno Betschart ∗ ∗Author correspondence. Transactions The Royal Society Medicine and Hygiene, Volume 88, Issue 4, July-August 1994, Pages 487–488,...

This is the first report of successful endoscopic resection an esophageal liposarcoma, in which patient's main symptom was dysphagia as a nonspecific sign [1] [2]. The differential diagnosis this type lesion includes leiomyoma, lipoma, gastrointestinal stromal tumor (GIST), and sarcoma [2] [3] [4] [5] [6]. case liposarcoma within esophagus reported 1983 [7].

X-irradiated 5-nitrobarbituric acid, 5-nitroorotic and 5-nitro-4,6-dihydroxypyrimidine show distinctive ESR spectra with very anisotropic nitrogen coupling, essentially the same as 5-nitrouracil. The 5-nitrouracil radical has been identified an iminoxyl formed by a mechanism which abstracts oxygen from nitro group leaving unpaired electron coupling to of group. It is clear that these other nitropyrimidines yield similar radicals. only reported nitropyrimidine yields different structures...

With respect to molecular switches, initializing the quadricyclane (QC) norbornadiene (NBD) back-reaction by light is highly desirable. Our previous publication provided a unique solution for this purpose utilizing covalently bound C60 . In work, fundamental processes within these hybrids has been investigated. Variation of linker unit connecting NBD/QC moiety with fullerene core used as tool tune properties resulting hybrids. Utilizing Prato reaction, two unprecedented - having long-rigid...

Two crystal polymorphs of phenobarbital, melting points 175 deg C and 17# C, were x-irradiated in air at room temperature with doses 10 Mrads. ESR spectra recorded 5 intervals about each 3 orthogonal axes both 9.3 GHz 22.5 GHz. A comparative table is preserted the data obtained from two structures. The results essentially same, except that relative values proton hyperfine couplings different because ethyl group rotations bond to C(5). It tentatively suggested one factor important mechanism...

Gamma irradiation of 5-nitro-6-methyluracil at 77°K results in a free radical formed by hydrogen addition to an oxygen the nitro group. The identity was confirmed synthesis of15 N labeled and observing its ESR spectra.

1-Methyl-5-nitrouracil, 3-methyl-5-nitrouracil, and 1,3-dimethyl-5-nitrouracil form iminoxy radicals R=N-Ȯ when exposed to ionizing radiation as do most other nitropyrimidines. The nitrogen hyperfine coupling principal values for this radical in 1-methyl-5-nitrouracil are $A_{1}=49{\rm G},A_{2}=29{\rm G}$, $A_{3}=25{\rm G}$; the g-value $g_{u}=2.0067,g_{v}=2.0059,g_{w}=2.0020$. Additional couplings seen at a few orientations allowed identification of complete structure. mechanism postulated...