A5070750713- Biofuel production and bioconversion
- Fungal and yeast genetics research
- Microbial Metabolic Engineering and Bioproduction
- Plant Pathogens and Fungal Diseases
- Mycotoxins in Agriculture and Food
- Enzyme Production and Characterization
- Polysaccharides and Plant Cell Walls
- Polyamine Metabolism and Applications
- Plant-Microbe Interactions and Immunity
- Fungal Biology and Applications
- Plant nutrient uptake and metabolism
- Enzyme Catalysis and Immobilization
- Amino Acid Enzymes and Metabolism
- Enzyme-mediated dye degradation
- Diet, Metabolism, and Disease
- Studies on Chitinases and Chitosanases
- Mycorrhizal Fungi and Plant Interactions
- Enzyme Structure and Function
- Microbial Metabolism and Applications
- RNA and protein synthesis mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Machine Learning in Bioinformatics
- Animal Genetics and Reproduction
- Bioeconomy and Sustainability Development
- Biopolymer Synthesis and Applications
TU Wien
2013-2025
Austrian Centre of Industrial Biotechnology (Austria)
2013-2018
Institute of Chemical Engineering
2017
Institute of Molecular Biotechnology
2002
University of Debrecen
2001
Abstract Background The ascomycete fungus, Trichoderma reesei (anamorph of Hypocrea jecorina ), represents a biotechnological workhorse and is currently one the most proficient cellulase producers. While strain improvement was traditionally accomplished by random mutagenesis, detailed understanding regulation can only be gained using recombinant technologies. Results Aiming at high efficiency throughput methods, we present here construction kit for gene knock out in T. . We provide primer...
Abstract Background Mycoparasitism, a lifestyle where one fungus is parasitic on another fungus, has special relevance when the prey plant pathogen, providing strategy for biological control of pests protection. Probably, most studied biocontrol agents are species genus Hypocrea / Trichoderma . Results Here we report an analysis genome sequences two atroviride (teleomorph atroviridis ) and virens (formerly Gliocladium , teleomorph ), comparison with reesei jecorina ). These three display...
Hypocrea jecorina (= Trichoderma reesei) is the main industrial source of cellulases and hemicellulases used to depolymerise plant biomass simple sugars that are converted chemical intermediates biofuels, such as ethanol. Cellulases formed adaptively, several positive (XYR1, ACE2, HAP2/3/5) negative (ACE1, CRE1) components involved in this regulation now known. In addition, its complete genome sequence has been recently published, thus making organism susceptible targeted improvement by...
Genome‐wide analysis of chitinase genes in the Hypocrea jecorina (anamorph: Trichoderma reesei ) genome database revealed presence 18 ORFs encoding putative chitinases, all them belonging to glycoside hydrolase family 18. Eleven these encode yet undescribed chitinases. A systematic nomenclature for H. chitinases is proposed, which designates corresponding their and numbers isoenzymes according pI from Chi18‐1 Chi18‐18. Phylogenetic those other filamentous fungi, including hypothetical...
Abstract Background The identification and characterization of the transcriptional regulatory networks governing physiology adaptation microbial cells is a key step in understanding their behaviour. One such wide-domain circuit, essential to all cells, carbon catabolite repression (CCR): it allows cell prefer some sources, whose assimilation high nutritional value, over less profitable ones. In lower multicellular fungi, C2H2 zinc finger CreA/CRE1 protein has been shown act as repressor this...
Trichoderma reesei is an industrial producer of enzymes that degrade lignocellulosic polysaccharides to soluble monomers, which can be fermented biofuels. Here we show the expression genes for lignocellulose degradation are controlled by orthologous T. protein methyltransferase LAE1. In a lae1 deletion mutant observed complete loss all seven cellulases, auxiliary factors cellulose degradation, β-glucosidases and xylanases were no longer expressed. Conversely, enhanced resulted in...
Trichoderma reesei (teleomorph Hypocrea jecorina) is the main industrial source of cellulases and hemicellulases harnessed for hydrolysis biomass to simple sugars, which can then be converted biofuels such as ethanol other chemicals. The highly productive strains in use today were generated by classical mutagenesis. To learn how cellulase production was improved these techniques, we performed massively parallel sequencing identify mutations genomes two hyperproducing (NG14, its direct...
Abstract Background The hypercellulolytic mutant Hypocrea jecorina (anamorph Trichoderma reesei ) RUT C30 is the H. strain most frequently used for cellulase fermentations and has also often been employed basic research on regulation. This reported to contain a truncated carbon catabolite repressor gene cre1 consequently derepressed. To date this an additional frame-shift mutation in glycoprotein-processing β-glucosidase II encoding are only known genetic differences C30. Results In present...
Due to its capacity produce large amounts of cellulases, Trichoderma reesei is increasingly being investigated for second-generation biofuel production from lignocellulosic biomass. The induction mechanisms T. cellulases have been described recently, but the regulation genes involved in their transcription has not studied thoroughly. Here we report expression two activator xyr1 and ace2, corepressor gene ace1, during cellulase biosynthesis by inducer lactose QM 9414, a strain producing low...
Trichoderma reesei colonizes predecayed wood in nature and metabolizes cellulose hemicellulose from the plant biomass. The respective enzymes are industrially produced for application biofuel biorefinery industry. However, these also induced presence of lactose (1,4-0-ß-d-galactopyranosyl-d-glucose), a waste product cheese manufacture or whey processing industries. In fact, is only soluble carbon source that induces T. on an industrial level but reason this unique phenomenon not understood....
Renewable lignocellulosic biomass is an advantageous resource for the production of second generation biofuels and other biorefinery products. In Middle Europe, wheat straw one most abundant low-cost sources biomass. For its efficient use, mix cellulases hemicellulases required. this paper, we investigated how cellulase by T. reesei on compares to that lactose, only soluble also cheap inducing carbon source enzyme production.We have examined compared transcriptome growing lactose as under...
Abstract The putative methyltransferase LaeA is a global regulator that affects the expression of multiple secondary metabolite gene clusters in several fungi, and it can modify heterochromatin structure Aspergillus nidulans. We have recently shown ortholog Trichoderma reesei (LAE1), fungus an industrial producer cellulase hemicellulase enzymes, regulates cellulases polysaccharide hydrolases. To learn more about function LAE1 T. reesei, we assessed effect deletion overexpression lae1 on...
ABSTRACT The ascomycete Hypocrea jecorina ( Trichoderma reesei ), an industrial producer of cellulases and hemicellulases, can efficiently degrade plant polysaccharides. However, the catabolic pathways for resulting monomers their relationship to enzyme induction are not well known. Here we used Biolog Phenotype MicroArrays technique evaluate growth H. on 95 carbon sources. For this purpose, compared several wild-type isolates, mutants producing different amounts cellulases, strains...
Summary The Hypocrea jecorina d ‐xylose reductase encoding gene xyl1 shows low basal transcript levels, and is induced by ‐xylose, l ‐arabinose ‐arabinitol and, to a lesser extent, lactose, ‐galactose, galactitol xylitol. recombinantly expressed XYL1 catalyzes the NADPH‐dependent reduction of pentoses hexose ‐galactose. Deletion slightly reduces growth on all carbon sources, but significant decrease found Similar pentose degradation, ‐galactose in recently identified second pathway. Strains...
Trichoderma reesei is a model for investigating the regulation of (hemi-)cellulase gene expression. Cellulases are formed adaptively, and transcriptional activator XYR1 carbon catabolite repressor CRE1 main regulators their We quantified nucleo-cytoplasmic shuttling dynamics GFP-fusion proteins both transcription factors under cellulase xylanase inducing conditions, correlated nuclear presence/absence with changes. also compared subcellular localization in conidial germlings mature hyphae....
Summary We have investigated the effect of disruption bgl1 ‐(β‐glucosidase l‐encoding) gene Trichoderma reesei on formation other β‐glucosidase activities and induction cellulases. To this end locus was disrupted by insertion Aspergillus nidulans amdS (acetamidase‐encoding) gene. The ‐disrupted strain did not produce 75kDa extracellular cellulose or lactose, but still formed activity glucose, cellobiose, xylan β‐1,3‐glucan, suggesting that enzyme(s) exhibiting is (are) encoded bgl1....
The conidium plays a critical role in the life cycle of many filamentous fungi, being primary means for survival under unfavorable conditions. To investigate transcriptional changes taking place during transition from growing hyphae to conidia Trichoderma reesei, microarray experiments were performed. A total 900 distinct genes classified as differentially expressed, relative their expression at time zero conidiation, least one points analyzed. main functional categories (FunCat)...
Recent demands for the production of biofuels from lignocellulose led to an increased interest in engineered cellulases Trichoderma reesei or other fungal sources. While methods generate such mutant on DNA level are straightforward, there is often a bottleneck their since correct posttranslational processing these enzymes needed obtain highly active enzymes. Their and subsequent enzymatic analysis homologous host T. is, however, disturbed by concomitant endogenous cellulases. As useful...
Hypocrea jecorina (anamorph: Trichoderma reesei) can grow on plant arabinans by the aid of secreted arabinan-degrading enzymes. This growth arabinan and its degradation product L-arabinose requires operation aldose reductase XYL1 L-arabinitol dehydrogenase LAD1. Growth is also severely affected in a strain deficient general cellulase hemicellulase regulator XYR1, but this impairment be overcome constitutive expression xyl1 encoding reductase. An inspection genome H. reveals four genes...