A5052937095- Epigenetics and DNA Methylation
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Ferroptosis and cancer prognosis
- IL-33, ST2, and ILC Pathways
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
IRCCS Humanitas Research Hospital
2022-2023
Humanitas University
2019-2022
Istituti di Ricovero e Cura a Carattere Scientifico
2020
microRNAs (miRNAs) modulate gene expression by repressing translation of targeted genes. Previous work has established a role for miRNAs in regulating vascular smooth muscle cell (VSMC) activity. Whether circular RNAs are involved the modulation miRNA activity VSMCs is unknown.We aimed to identify interacting with enriched and modulating cells' activity.RNA sequencing bioinformatics identified several VSMCs; however, only one, possessing multiple putative binding sites miR-145, was highly...
MicroRNAs (miRNAs, miRs) are small noncoding RNAs that modulate gene expression by negatively regulating translation of target genes. Although the role several miRNAs in vascular smooth muscle cells (VSMCs) has been extensively characterized, function miRNA-128-3p (miR-128) is still unknown.To determine if miR-128 modulates VSMC phenotype and to define underlying mechanisms.We screened for whose modulated an altered DNA methylation status VSMCs, among hits, we selected miR-128. We found was...
Histone H3 dimethylation at lysine 79 is a key epigenetic mark uniquely induced by methyltransferase disruptor of telomeric silencing 1-like (DOT1L). We aimed to determine whether DOT1L modulates vascular smooth muscle cell (VSMC) phenotype and how it might affect atherosclerosis in vitro vivo, unravelling the related mechanism.Gene expression screening VSMCs stimulated with BB isoform platelet-derived growth factor led us identify Dot1l as an early up-regulated factor. Mouse human...