A5086811873- Protein Structure and Dynamics
- Material Dynamics and Properties
- Spectroscopy and Quantum Chemical Studies
- Enzyme Structure and Function
- Advanced NMR Techniques and Applications
- Photoreceptor and optogenetics research
- Receptor Mechanisms and Signaling
- Quantum, superfluid, helium dynamics
- Nuclear Physics and Applications
- NMR spectroscopy and applications
- Mass Spectrometry Techniques and Applications
- Nanopore and Nanochannel Transport Studies
- Phase Equilibria and Thermodynamics
- Molecular spectroscopy and chirality
- Theoretical and Computational Physics
- Neuroscience and Neuropharmacology Research
- ATP Synthase and ATPases Research
- Magnetic and Electromagnetic Effects
- Algal biology and biofuel production
- Force Microscopy Techniques and Applications
- Methane Hydrates and Related Phenomena
- Protein Interaction Studies and Fluorescence Analysis
- Experimental and Theoretical Physics Studies
- Hydrocarbon exploration and reservoir analysis
- RNA and protein synthesis mechanisms
City University of Hong Kong
2023-2025
City University of Hong Kong, Shenzhen Research Institute
2023-2025
Tianjin Medical University Cancer Institute and Hospital
2024
China Academy of Engineering Physics
2018-2021
Institute of High Energy Physics
2021
Chinese Academy of Sciences
2021
Australian Nuclear Science and Technology Organisation
2021
Shanghai Advanced Research Institute
2021
Shanghai Zhangjiang Laboratory
2021
University of Chinese Academy of Sciences
2021
We used high-resolution quasielastic neutron scattering spectroscopy to study the single-particle dynamics of water molecules on surface hydrated DNA samples. Both H2O and D2O samples were measured. The contribution from is subtracted out by taking difference signals between two measurement was made at a series temperatures 270 K down 185 K. Relaxing-Cage Model analyze spectra. This allowed us extract Q-independent average translational relaxation time as function temperature. observe clear...
The scattering of neutrons can be used to provide information on the structure and dynamics biological systems multiple length time scales. Pursuant a National Science Foundation-funded workshop in February 2018, recent developments this field are reviewed here, as well future prospects that expected given advances sources, instrumentation computational power methods. Crystallography, solution scattering, dynamics, membranes, labeling imaging examined. For extraction maximum information,...
A super-Arrhenius-to-Arrhenius dynamic crossover phenomenon has been observed in the translational α-relaxation time and inverse of self-diffusion constant both experimentally by simulations for lysozyme hydration water temperature range TL = 223 ± 2 K. MD are based on a realistic hydrated powder model, which uses TIP4P-Ew rigid molecular model water. The convergence neutron scattering, nuclear magnetic resonance dynamics supports interpretation that this is result gradual evolution...
High-resolution quasielastic neutron scattering spectroscopy was used to measure ${\mathrm{H}}_{2}\mathrm{O}$ hydrated double-wall carbon nanotubes (DWNT). The measurements were made at a series of temperatures from $250\phantom{\rule{0.3em}{0ex}}\mathrm{K}$ down $150\phantom{\rule{0.3em}{0ex}}\mathrm{K}$. relaxing-cage model analyze the spectra. We observed clear evidence fragile-to-strong dynamic crossover (FSC) ${T}_{L}=190\phantom{\rule{0.3em}{0ex}}\mathrm{K}$ in confined water. further...
High-resolution quasielastic neutron scattering spectroscopy was used to measure ${\text{H}}_{2}\text{O}$ and ${\text{D}}_{2}\text{O}$ hydrated RNA samples. The contribution of from subtracted out by taking the difference signals between two measurements were made at a series temperatures 270 K down 180 K. relaxing-cage model analyze spectra. We observed clear evidence fragile-to-strong dynamic crossover (FSC) ${T}_{L}=220\text{ }\text{K}$ in hydration water. further show that mean-square...
Molecular dynamics simulations and neutron scattering experiments have shown that many hydrated globular proteins exhibit a universal dynamic transition at ${T}_{D}=220\text{ }\text{ }\mathrm{K}$, below which the biological activity of protein sharply diminishes. We studied phononlike low-energy excitations two structurally very different proteins, lysozyme bovine serum albumin, using inelastic x-ray above ${T}_{D}$. found excitation energies high-$Q$ phonons show marked softening This...
Both the structure and dynamics of biomolecules are known to be essential for their biological function. In dehydrated state, function biomolecules, such as proteins, is severely impeded, so hydration required bioactivity. The hydrated water related – but how closely? problem involves several layers complexity. Even in bulk contribution from various dynamic components overall not fully understood. systems, effects confinement on further complicate picture. if properly understood, which them...
This study develops a generalized method for applying spin–echo small-angle neutron scattering (SESANS) to the structural analysis of polymers. Starting from theoretical framework SESANS, we developed real space correlation functions Gaussian chain model systems consisting chains with many beads. Further molecular dynamics (MD) simulations affirm that derived by our proposed work can accurately predict radii gyration polymer chains, which bring straightforward insight SESANS measurements....
The low-temperature behavior of proteins under high pressure is not as extensively investigated that at ambient pressure. In this paper, we study the dynamics a hydrated protein moderately pressures low temperatures using quasielastic neutron scattering method. We show when applying to protein−water system, hydration water does slow down but becomes faster instead. degree "softness" protein, which intimately related enzymatic activity shows same trend its function temperature different...
Significance Deep-sea microorganisms can adapt to extreme conditions of high temperature and pressure. What makes these organisms survive reproduce in such critical remains an open question. Here, we use the quasielastic neutron scattering (QENS) technique study dynamic behavior a hyperthermophilic protein that is found deep sea. Our results give evidence pressure affects dynamical properties proteins by distorting energy landscape ways are significantly different for mesophilic proteins....
Light activation of the visual G-protein-coupled receptor (GPCR) rhodopsin leads to significant structural fluctuations protein embedded within membrane yielding cognate G-protein (transducin), which initiates biological signaling. Here, we report a quasi-elastic neutron scattering study as GPCR prototype. Our results reveal broadly distributed relaxation hydrogen atom dynamics on picosecond-nanosecond time scale, crucial for function, only observed globular proteins previously....
Experiments in bulk water confirm the existence of two local arrangements molecules with different densities, but, because inevitable freezing at low temperature $T$, can not ascertain whether separate phases. To avoid freezing, new experiments measure dynamics $T$ on surface proteins, finding a crossover from non-Arrhenius regime high to that is approximately Arrhenius $T$. Motivated by these experiments, Kumar et al. [Phys. Rev. Lett. 100, 105701 (2008)] investigated, Monte Carlo...
This review article describes our neutron scattering experiments made in the past four years for understanding of single-particle (hydrogen atom) dynamics a protein and its hydration water strong coupling between them. We found that key to this is existence fragile-to-strong dynamic crossover (FSC) phenomenon occurring at around TL = 225±5 K water. On lowering temperature toward FSC, structure makes transition from predominantly high density form (HDL), more fluid state, low (LDL), less...
We observe a logarithmic-like decay of the intermediate scattering function (ISF) hydrogen atoms in protein molecule time interval from 10 ps to 1 ns. analyze ISF, FH(Q,t), terms an asymptotic expression proposed by mode coupling theory (MCT). The result clearly shows that this logarithmic stretching β-relaxation range is real, substantiating prediction molecular dynamics (MD) simulation results used formula MCT for analysis ISF.
Studies of the low-temperature dynamics proteins in aqueous solutions are limited by crystallization water. In this work, we use a solution LiCl D2O as solvent for protein to prevent and study both its quasielastic neutron scattering (QENS) temperature range 210 290 K. Our results reveal that, while undergoes crossover at about 220 K, itself shows no transition temperature. The prevailing view is that β-fluctuations governed α-fluctuations solvent; therefore, observation apparent decoupling...
This review article describes our neutron scattering experiments made in the past four years for understanding of single-particle (hydrogen atom) dynamics a protein and its hydration water strong coupling between them. We found that key to this is existence fragile-to-strong dynamic crossover (FSC) phenomenon occurring at around T L = 225±5 K water. On lowering temperature toward FSC, structure makes transition from predominantly high density form (HDL), more fluid state, low (LDL), less...
Quasielastic neutron scattering was used to study the dynamics of three-dimensional confined water in a hydrophobic mesoporous material designated as CMK-1 temperature range from 250 170 K. We observe crossover phenomenon at T(L) . find that occurs between previous observations one-dimensional materials with different hydrophilicities. This provides first evidence besides obvious surface effect brought about by confinements, is also dependent on dimensionality geometry confinement.
The dynamics of RNA within the β-relaxation region 10 ps to 1 ns is crucial its biological function. Because simpler chemical building blocks and lack side methyl groups, faster relaxational compared proteins can be expected. However, situation actually opposite. In this work, tRNA measured by quasielastic neutron scattering analyzed using mode coupling theory, originally developed for glass-forming liquids. Our results reveal that follows a log-decay region, which an important trait...
Views Icon Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Twitter Facebook Reddit LinkedIn Tools Reprints and Permissions Cite Search Site Citation Sow‐Hsin Chen, F. Mallamace, L. Liu, D. Z. X. Q. Chu, Y. Zhang, C. Kim, A. Faraone, C.‐Y. Mou, E. Fratini, P. Baglioni, I. Kolesnikov, V. Garcia‐Sakai; Dynamic Crossover Phenomenon in Confined Supercooled Water Its Relation to the Existence of a Liquid‐Liquid Critical Point Water. AIP Conf. Proc. 21 February...
β-Casein is a component of casein micelle with amphillic nature and recognized as "natively disordered" protein that lacks secondary structures. In this study, the temperature hydration effects on dynamics β-casein are explored by quasielastic neutron scattering (QENS). An upturn in mean square displacement (MSD) hydrated indicates an increase flexibility at ~225 K. Another MSD ~100 K, observed both dry β-casein, ascribed to methyl group rotations, which not sensitive hydration. QENS...
Nontoxic, biocompatible nanodiamonds (ND) have recently been implemented in rational, systematic design of optimal therapeutic use nanomedicines. However, hydrophilicity the ND surface strongly influences structure and dynamics biomolecules that restrict situ applications ND. Therefore, fundamental understanding impact hydrophilic on at molecular level is essential. For tRNA, we observe an enhancement dynamical behavior presence contrary to generally observed slow motion interacting...
Abstract In total, 366 birds representing 55 species in 24 families and eight orders, were examined for chewing lice (Phthiraptera: Amblycera, Ischnocera) two high‐altitude localities Yunnan Province, China. Ailaoshan, almost all of the resident passeriforms, which 36% parasitized by lice. Jinshanyakou, most on migration, included both passerine non‐passerine birds. Of caught only one bird (0.7%) was The prevalence Myrsidea Brueelia ‐complex Ailaoshan higher than previous reports....
Soft matter systems exhibit diversity and intricacy in their structures properties, with dynamic behaviors structural changes spanning wide time length scales. Gaining insight into the internal dynamics of soft systems, as well interactions among molecules particles, contributes to a deeper comprehension microscopic matter. Moreover, this endeavor has significant biomedical materials engineering implications. This review focuses on applications spin-echo small-angle neutron scattering...
The purpose of this investigation is to determine whether a large oligomeric protein, inorganic pyrophosphatase (IPPase) from Thermococcus thioreducens with quaternary structural complexity, would have distinguishable dynamic characteristics compared those the small simple monomeric model lysozyme. In study, β-relaxational dynamics two proteins, IPPase and lysozyme, are in 10 ps 0.5 ns time interval using quasi-elastic neutron scattering (QENS). Both protein show characteristic...